Detailed information NTG0435366

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: HEQ84_RS04505
• Genome accession: NZ_CP050870
• Coordinates: 848487..849326 (+)
• Length: 279 a.a.
• NCBI ID: WP_011225887.1
• Uniprot ID: Q5M4N4
• Organism: Streptococcus thermophilus strain CS6
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 843487..854326

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HEQ84_RS04475 (HEQ84_04435)	-	843902..844429 (+)	528	WP_014608250.1	ECF transporter S component	-
HEQ84_RS04480 (HEQ84_04440)	-	844453..844968 (+)	516	WP_014608251.1	transcription repressor NadR	-
HEQ84_RS04485 (HEQ84_04445)	-	845063..845752 (+)	690	WP_023909608.1	helix-turn-helix transcriptional regulator	-
HEQ84_RS04490 (HEQ84_04450)	ylqF	845880..846734 (+)	855	WP_002950602.1	ribosome biogenesis GTPase YlqF	-
HEQ84_RS04495 (HEQ84_04455)	-	846721..847488 (+)	768	WP_024704092.1	ribonuclease HII	-
HEQ84_RS04500 (HEQ84_04460)	-	847499..848419 (+)	921	WP_011681085.1	ROK family protein	-
HEQ84_RS04505 (HEQ84_04465)	dprA	848487..849326 (+)	840	WP_011225887.1	DNA-processing protein DprA	Machinery gene
HEQ84_RS04510 (HEQ84_04470)	topA	849494..851638 (+)	2145	WP_014608256.1	type I DNA topoisomerase	-
HEQ84_RS04515 (HEQ84_04475)	-	851755..852579 (+)	825	WP_014608257.1	TraX family protein	-
HEQ84_RS04520 (HEQ84_04480)	-	852615..853274 (+)	660	WP_011227158.1	response regulator transcription factor	-

Sequence

Protein

• Length: 279 a.a.
• Molecular weight: 31247.18 Da
• Isoelectric Point: 8.6571

>NTDB_id=435366 HEQ84_RS04505 WP_011225887.1 848487..849326(+) (dprA) [Streptococcus thermophilus strain CS6]
MNNFELFKLKAAGLTNLNILNILDYQKNQDKKLSLRDMAVVSKSKNAVLFMEKYKNLDVKQLKEDFQRFPTLSIFDKEYP
IELKHSYNPPVLLFYQGNIDLLSKPKLAVVGARKCSETGKQSVRKIVHELGNAFTIVSGLARGIDTCAHMEALKNKGNTI
AVIGTGLDVYYPKENKALQDYMAKNHLVLTEYGPGEQPLKYHYPERNRIISGLCQGVMVVEAKLRSGSLITCERAMEEGR
DVFAIPGNILDGKSDGCHHLIQEGAKCITTGFDIISEFS

Nucleotide

• Length: 840 bp

>NTDB_id=435366 HEQ84_RS04505 WP_011225887.1 848487..849326(+) (dprA) [Streptococcus thermophilus strain CS6]
ATGAATAACTTTGAACTTTTTAAACTAAAAGCTGCTGGATTGACGAATTTAAACATCCTTAACATTTTAGACTATCAAAA
AAATCAAGATAAAAAATTGAGCTTACGTGATATGGCAGTGGTATCTAAGTCTAAAAATGCCGTTCTCTTTATGGAAAAAT
ACAAGAATTTGGATGTTAAGCAGCTTAAGGAAGATTTTCAACGTTTTCCGACCTTATCAATTTTTGATAAGGAGTATCCC
ATTGAGCTGAAACATTCCTATAATCCTCCTGTTCTCCTCTTTTATCAAGGGAATATTGATTTGCTAAGTAAACCCAAGTT
AGCAGTAGTTGGTGCCAGAAAGTGTTCTGAAACAGGCAAACAGTCTGTTCGAAAAATTGTTCATGAACTTGGGAATGCCT
TTACTATTGTAAGTGGCTTGGCGCGTGGAATTGATACTTGTGCTCATATGGAGGCCTTAAAAAATAAAGGTAACACCATT
GCAGTTATCGGGACTGGGCTAGACGTATATTATCCTAAAGAAAACAAAGCTTTACAGGACTATATGGCTAAAAATCATTT
AGTTCTTACAGAATACGGACCGGGTGAACAGCCACTTAAATACCATTATCCTGAACGAAATCGGATAATTTCTGGATTGT
GTCAAGGGGTTATGGTGGTTGAAGCCAAGCTTCGCTCGGGAAGTCTTATTACTTGTGAGCGTGCAATGGAAGAAGGAAGA
GATGTTTTCGCCATACCAGGAAATATTTTAGATGGAAAATCGGATGGATGCCATCACCTCATTCAAGAGGGCGCAAAGTG
CATTACTACAGGATTTGACATCATTTCAGAATTTTCATAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (71-269)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-62)

3D structure

• AlphaFold DB: Q5M4N4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	72.043	100	0.72
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae D39	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae R6	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	62.95	99.642	0.627
dprA/cilB/dalA	Streptococcus mitis SK321	62.95	99.642	0.627
dprA	Lactococcus lactis subsp. cremoris KW2	60.432	99.642	0.602
dprA	Latilactobacillus sakei subsp. sakei 23K	39.768	92.832	0.369
dprA	Bacillus subtilis subsp. subtilis str. 168	48.095	75.269	0.362