Detailed information NTG0043423

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BACAU_RS12425
• Genome accession: NC_016784
• Coordinates: 2600409..2601023 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis CAU B946
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2595409..2606023

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BACAU_RS12400 (BACAU_2397)	rpsT	2595709..2595975 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BACAU_RS12405 (BACAU_2398)	holA	2595992..2597033 (-)	1042	Protein_2461	DNA polymerase III subunit delta	-
BACAU_RS20605	-	2597073..2597227 (-)	155	Protein_2462	hypothetical protein	-
BACAU_RS20180 (BACAU_2399)	-	2597268..2597402 (+)	135	WP_041481886.1	YqzM family protein	-
BACAU_RS12415 (BACAU_2400)	comEC	2597421..2599772 (-)	2352	WP_041481887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BACAU_RS12420 (BACAU_2401)	-	2599773..2600342 (-)	570	WP_003152868.1	ComE operon protein 2	-
BACAU_RS12425 (BACAU_2402)	comEA	2600409..2601023 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
BACAU_RS12430 (BACAU_2403)	comER	2601082..2601903 (+)	822	WP_014305449.1	late competence protein ComER	-
BACAU_RS12435 (BACAU_2404)	-	2601972..2602709 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
BACAU_RS12440 (BACAU_2405)	rsfS	2602706..2603062 (-)	357	WP_014305451.1	ribosome silencing factor	-
BACAU_RS12445 (BACAU_2406)	yqeK	2603059..2603640 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BACAU_RS12450 (BACAU_2407)	-	2603630..2604199 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
BACAU_RS12455 (BACAU_2408)	yhbY	2604210..2604500 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BACAU_RS12460 (BACAU_2409)	aroE	2604494..2605336 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=43423 BACAU_RS12425 WP_003152867.1 2600409..2601023(-) (comEA) [Bacillus velezensis CAU B946]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=43423 BACAU_RS12425 WP_003152867.1 2600409..2601023(-) (comEA) [Bacillus velezensis CAU B946]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363