Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HBA21_RS23490 Genome accession   NZ_CP050148
Coordinates   5078687..5080387 (+) Length   566 a.a.
NCBI ID   WP_122359259.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain AA43     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5073687..5085387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBA21_RS23465 (HBA21_23515) ampD 5073724..5074290 (-) 567 WP_003104911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HBA21_RS23470 (HBA21_23520) - 5074441..5076696 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  HBA21_RS23475 (HBA21_23525) nadC 5076982..5077830 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HBA21_RS23485 (HBA21_23535) - 5077983..5078456 (-) 474 WP_015503553.1 pilin -
  HBA21_RS23490 (HBA21_23540) pilB 5078687..5080387 (+) 1701 WP_122359259.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HBA21_RS23495 (HBA21_23545) pilC 5080391..5081608 (+) 1218 WP_010792214.1 type II secretion system F family protein Machinery gene
  HBA21_RS23500 (HBA21_23550) pilD 5081611..5082483 (+) 873 WP_023911898.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HBA21_RS23505 (HBA21_23555) coaE 5082480..5083091 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  HBA21_RS23510 (HBA21_23560) yacG 5083088..5083288 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  HBA21_RS23515 (HBA21_23565) - 5083325..5083534 (-) 210 WP_003094660.1 hypothetical protein -
  HBA21_RS23520 (HBA21_23570) - 5083640..5084329 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  HBA21_RS23525 (HBA21_23575) - 5084326..5084796 (-) 471 WP_023911895.1 hypothetical protein -
  HBA21_RS23530 (HBA21_23580) - 5084793..5085218 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62384.31 Da        Isoelectric Point: 6.0138

>NTDB_id=430380 HBA21_RS23490 WP_122359259.1 5078687..5080387(+) (pilB) [Pseudomonas aeruginosa strain AA43]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSAEKSTQEDTSAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAKQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=430380 HBA21_RS23490 WP_122359259.1 5078687..5080387(+) (pilB) [Pseudomonas aeruginosa strain AA43]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTATCCGCCGAGCAGTTCGGTATCGCCTACTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAGCACCGGGTCATCCCTTTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGATCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAACTGTTCGAAAGCGCTACCGATGGTCTCGCAGGCCTCGACGATGTTGACCTCGAG
GGACTGGATATCGGTAGCGCGGAAAAGTCCACCCAGGAGGATACCAGCGCAGAAGCGGACGACGCTCCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATAAAGGGTGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGACATTTCCGAACGGCGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGAGCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAAATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGACATATGGTGATGTCCACCCTACACACCAACAGCGCCGCCGA
GACCCTGACTCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTTCCGGAA
GACAAGATCGGTACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCAAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.692

100

0.583
  pilB Acinetobacter baylyi ADP1

57.371

99.47

0.571
  pilB Legionella pneumophila strain ERS1305867

55.3

100

0.553
  pilB Vibrio parahaemolyticus RIMD 2210633

51.241

99.647

0.511
  pilF Neisseria gonorrhoeae MS11

50.712

99.293

0.504
  pilB Vibrio cholerae strain A1552

50.265

99.823

0.502
  pilB Vibrio campbellii strain DS40M4

50

99.647

0.498
  pilF Thermus thermophilus HB27

39.292

99.823

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.607

93.11

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

38.563

93.463

0.36