Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HBA48_RS05860 Genome accession   NZ_CP050135
Coordinates   1113584..1114768 (+) Length   394 a.a.
NCBI ID   WP_003790159.1    Uniprot ID   -
Organism   Kingella kingae strain ATCC 23331     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1108584..1119768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBA48_RS05840 (HBA48_05870) - 1108892..1110343 (-) 1452 WP_003790163.1 sodium:proton antiporter -
  HBA48_RS05845 (HBA48_05875) - 1110466..1111311 (-) 846 WP_003790161.1 PHP domain-containing protein -
  HBA48_RS05850 (HBA48_05880) - 1111474..1112814 (-) 1341 WP_166503237.1 DUF3482 domain-containing protein -
  HBA48_RS05855 (HBA48_05885) - 1112920..1113402 (-) 483 WP_003786169.1 Lrp/AsnC family transcriptional regulator -
  HBA48_RS05860 (HBA48_05890) pilU 1113584..1114768 (+) 1185 WP_003790159.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HBA48_RS05865 (HBA48_05895) - 1114851..1115606 (+) 756 WP_003790157.1 sulfite exporter TauE/SafE family protein -
  HBA48_RS05870 (HBA48_05900) - 1115621..1116151 (+) 531 WP_003790154.1 CreA family protein -
  HBA48_RS05875 (HBA48_05905) hisI 1116161..1116556 (+) 396 WP_003790152.1 phosphoribosyl-AMP cyclohydrolase -
  HBA48_RS05880 (HBA48_05910) - 1116584..1116913 (+) 330 WP_003790151.1 phosphoribosyl-ATP diphosphatase -
  HBA48_RS05885 (HBA48_05915) - 1116985..1117305 (+) 321 WP_003790149.1 histidine triad nucleotide-binding protein -
  HBA48_RS05890 (HBA48_05920) tatA 1117357..1117560 (+) 204 WP_003786162.1 Sec-independent protein translocase subunit TatA -
  HBA48_RS05895 (HBA48_05925) tatB 1117562..1118068 (+) 507 WP_038310885.1 Sec-independent protein translocase protein TatB -
  HBA48_RS05900 (HBA48_05930) tatC 1118065..1118829 (+) 765 WP_003786159.1 twin-arginine translocase subunit TatC -

Sequence

Protein


Download         Length: 394 a.a.        Molecular weight: 43718.21 Da        Isoelectric Point: 6.0102

>NTDB_id=430263 HBA48_RS05860 WP_003790159.1 1113584..1114768(+) (pilU) [Kingella kingae strain ATCC 23331]
MQVIIPPINEEMRKFIHGLLTHMVNNKGSDLFITAGYPPAMKLDGKLTKITDKPLTADHAMQIARSIMDDKQAEEFLTTN
ECNFAISLSGVSRFRINAMVQRGAAALVCRVITSDIPKFDNMNLPPVLKQVVMEKRGLVIFVGGTGSGKSTSLAAMIDYR
NENSYGHIITIEDPIEFVHPHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCMAT
LHANNSNQALDRIINFFPEERRTQLLNDLSLNLKGFISQRLVPKKQGKGRVAAVEILLNSPLISELVLHGDIHAVKEIMA
KSRDIGMQTFDQSLFDLYEADLISYDDALRNADSVNDLRLQIQLNSKKNATAGSGGVLDGLSLTDYAPPEAEEK

Nucleotide


Download         Length: 1185 bp        

>NTDB_id=430263 HBA48_RS05860 WP_003790159.1 1113584..1114768(+) (pilU) [Kingella kingae strain ATCC 23331]
ATGCAAGTTATCATTCCACCCATCAACGAAGAAATGCGTAAATTTATCCATGGCTTGCTCACGCACATGGTCAATAACAA
AGGCTCGGATTTATTTATCACGGCTGGCTATCCACCTGCGATGAAATTGGACGGCAAATTAACCAAAATCACCGACAAGC
CTTTAACCGCCGACCACGCCATGCAAATTGCGCGTTCGATTATGGACGACAAGCAAGCCGAAGAATTTTTAACCACCAAC
GAATGCAATTTTGCGATTAGCTTGTCAGGCGTGTCGCGCTTTCGTATCAACGCCATGGTGCAACGTGGTGCAGCAGCTTT
GGTTTGCCGTGTGATTACCAGCGACATTCCCAAATTCGACAACATGAACTTACCGCCTGTGTTAAAACAAGTCGTGATGG
AAAAACGTGGTTTGGTGATTTTTGTCGGCGGTACAGGTTCGGGTAAATCCACATCGTTGGCGGCGATGATTGATTACCGC
AACGAAAATAGCTACGGTCATATCATCACGATTGAAGACCCGATTGAATTTGTTCACCCACACAAAAACTGCATCATCAC
GCAACGCGAAGTGGGTGTGGACACCGAAAACTGGTTCGCCGCGCTCAAAAACACACTGCGCCAAGCACCCGATGTGATTT
TGATTGGTGAGATTCGTGACCGCGAAACGATGGACTACGCACTTGCCTTTGCCGAAACAGGGCACTTGTGTATGGCAACA
CTGCACGCGAATAACTCCAACCAGGCCCTTGACCGCATTATTAACTTCTTCCCCGAAGAACGCCGTACCCAGTTGCTGAA
CGACTTGTCGCTCAACTTAAAAGGCTTTATTTCGCAACGCCTTGTCCCCAAAAAACAAGGCAAAGGACGTGTGGCTGCGG
TAGAAATCCTGCTCAATTCGCCGTTGATTTCCGAATTGGTGTTGCATGGCGACATTCATGCGGTAAAAGAAATTATGGCA
AAATCGCGCGATATTGGTATGCAAACGTTCGACCAATCGCTGTTTGATTTGTATGAAGCCGATTTGATTTCGTATGACGA
TGCCTTGCGTAATGCTGACTCGGTAAACGATTTGCGCCTGCAAATTCAGTTAAACAGCAAGAAAAACGCAACGGCGGGTT
CGGGTGGCGTGCTGGACGGCTTGTCTTTGACGGATTACGCACCGCCAGAAGCCGAAGAAAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.523

89.34

0.523
  pilU Acinetobacter baylyi ADP1

58.239

89.34

0.52
  pilU Vibrio cholerae strain A1552

53.406

93.147

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.162

93.909

0.396
  pilT Legionella pneumophila strain Lp02

43.544

84.518

0.368
  pilT Legionella pneumophila strain ERS1305867

43.544

84.518

0.368