Detailed information
Bioinformatically predicted
Overview
| Name | pilU | Type | Machinery gene |
| Locus tag | G8D99_RS07710 | Genome accession | NZ_CP049916 |
| Coordinates | 1648318..1649487 (+) | Length | 389 a.a. |
| NCBI ID | WP_166324085.1 | Uniprot ID | A0A6G8S493 |
| Organism | Acinetobacter lanii strain 185 | ||
| Function | assembly of type IV pilus (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| ICE | 1620404..1663173 | 1648318..1649487 | within | 0 |
Gene organization within MGE regions
Location: 1620404..1663173
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G8D99_RS07585 (G8D99_07585) | tusD | 1620665..1621033 (-) | 369 | WP_166324032.1 | sulfurtransferase complex subunit TusD | - |
| G8D99_RS07590 (G8D99_07590) | galE | 1621106..1622110 (+) | 1005 | WP_166324034.1 | UDP-glucose 4-epimerase GalE | - |
| G8D99_RS07595 (G8D99_07595) | fumC | 1622320..1623714 (+) | 1395 | WP_166324036.1 | class II fumarate hydratase | - |
| G8D99_RS07600 (G8D99_07600) | - | 1623884..1624066 (+) | 183 | WP_166324038.1 | type II toxin-antitoxin system HicA family toxin | - |
| G8D99_RS07605 (G8D99_07605) | - | 1624116..1624520 (+) | 405 | WP_166324042.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| G8D99_RS07610 (G8D99_07610) | - | 1624798..1625289 (+) | 492 | WP_166324045.1 | hypothetical protein | - |
| G8D99_RS07615 (G8D99_07615) | - | 1625637..1626239 (+) | 603 | WP_166324049.1 | nitroreductase family protein | - |
| G8D99_RS07620 (G8D99_07620) | - | 1626402..1627634 (-) | 1233 | WP_166324052.1 | MFS transporter | - |
| G8D99_RS07625 (G8D99_07625) | - | 1627627..1628094 (-) | 468 | WP_166324055.1 | MarR family winged helix-turn-helix transcriptional regulator | - |
| G8D99_RS07630 (G8D99_07630) | - | 1628244..1629086 (-) | 843 | WP_166324057.1 | DUF805 domain-containing protein | - |
| G8D99_RS07635 (G8D99_07635) | - | 1629288..1629839 (-) | 552 | WP_166324059.1 | thermonuclease family protein | - |
| G8D99_RS07640 (G8D99_07640) | - | 1631021..1632259 (+) | 1239 | WP_166324061.1 | acyl-CoA dehydrogenase family protein | - |
| G8D99_RS07645 (G8D99_07645) | - | 1632410..1633531 (+) | 1122 | WP_166324063.1 | phosphotransferase family protein | - |
| G8D99_RS07650 (G8D99_07650) | - | 1633528..1634226 (+) | 699 | WP_166324065.1 | histidine phosphatase family protein | - |
| G8D99_RS07655 (G8D99_07655) | - | 1634289..1635032 (+) | 744 | WP_166324067.1 | SDR family oxidoreductase | - |
| G8D99_RS07660 (G8D99_07660) | - | 1635256..1636059 (-) | 804 | WP_227554377.1 | hypothetical protein | - |
| G8D99_RS07665 (G8D99_07665) | - | 1636287..1636997 (+) | 711 | WP_166327617.1 | SGNH/GDSL hydrolase family protein | - |
| G8D99_RS07670 (G8D99_07670) | - | 1637105..1637695 (+) | 591 | WP_166324069.1 | uracil-DNA glycosylase family protein | - |
| G8D99_RS07675 (G8D99_07675) | - | 1637771..1638208 (-) | 438 | WP_166324071.1 | hypothetical protein | - |
| G8D99_RS07680 (G8D99_07680) | - | 1638431..1639987 (-) | 1557 | WP_166324073.1 | phospholipase D family protein | - |
| G8D99_RS07685 (G8D99_07685) | - | 1640111..1641226 (+) | 1116 | WP_166324075.1 | S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase | - |
| G8D99_RS07690 (G8D99_07690) | - | 1641315..1642520 (-) | 1206 | WP_166324077.1 | amino acid aminotransferase | - |
| G8D99_RS07695 (G8D99_07695) | - | 1642841..1643623 (+) | 783 | WP_166324079.1 | alpha/beta fold hydrolase | - |
| G8D99_RS07700 (G8D99_07700) | - | 1644225..1646351 (+) | 2127 | WP_166324081.1 | EAL domain-containing protein | - |
| G8D99_RS07705 (G8D99_07705) | - | 1646892..1647941 (+) | 1050 | WP_166324083.1 | 3-deoxy-7-phosphoheptulonate synthase | - |
| G8D99_RS07710 (G8D99_07710) | pilU | 1648318..1649487 (+) | 1170 | WP_166324085.1 | PilT/PilU family type 4a pilus ATPase | Machinery gene |
| G8D99_RS07715 (G8D99_07715) | gcvH | 1649577..1649951 (-) | 375 | WP_166324087.1 | glycine cleavage system protein GcvH | - |
| G8D99_RS07720 (G8D99_07720) | - | 1650038..1651069 (-) | 1032 | WP_166324089.1 | AraC family transcriptional regulator | - |
| G8D99_RS07725 (G8D99_07725) | - | 1651163..1651618 (-) | 456 | WP_166324091.1 | MaoC family dehydratase | - |
| G8D99_RS07730 (G8D99_07730) | - | 1651620..1653470 (-) | 1851 | WP_166324093.1 | ABC transporter ATP-binding protein | - |
| G8D99_RS07735 (G8D99_07735) | - | 1653964..1654863 (-) | 900 | WP_166324095.1 | DUF4198 domain-containing protein | - |
| G8D99_RS07740 (G8D99_07740) | - | 1654968..1656545 (+) | 1578 | WP_227554378.1 | DUF2271 domain-containing protein | - |
| G8D99_RS07745 (G8D99_07745) | - | 1656565..1657155 (+) | 591 | WP_166324097.1 | PepSY-associated TM helix domain-containing protein | - |
| G8D99_RS07750 (G8D99_07750) | - | 1657169..1658665 (+) | 1497 | WP_166324100.1 | sulfite reductase subunit alpha | - |
| G8D99_RS07755 (G8D99_07755) | - | 1658722..1659261 (-) | 540 | WP_166324103.1 | DUF2058 domain-containing protein | - |
| G8D99_RS15910 | - | 1659651..1659830 (-) | 180 | WP_406741511.1 | hypothetical protein | - |
| G8D99_RS07765 (G8D99_07765) | sstT | 1659995..1661197 (-) | 1203 | WP_166324106.1 | serine/threonine transporter SstT | - |
| G8D99_RS07770 (G8D99_07770) | - | 1661385..1663115 (-) | 1731 | WP_166327621.1 | SulP family inorganic anion transporter | - |
Sequence
Protein
Download Length: 389 a.a. Molecular weight: 44060.28 Da Isoelectric Point: 6.6216
>NTDB_id=428640 G8D99_RS07710 WP_166324085.1 1648318..1649487(+) (pilU) [Acinetobacter lanii strain 185]
MYTAELLNEARNFMFRLLKNVFEHGGSDLFISADFPPSMKHQGMMKPLSSQALTGEKTRLFAYSLMNDKQRDEFERELEC
NFAISIPEVSRFRINVFQQQLNVGMVIRTITSEIPTFSKLKLPASLKDVIMEKRGLILVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMVTHREVGVDCHNWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTEDGNGRRAAVEIMLNTPLVSDVILKGNFHELKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEAIRNADSMNELRLKIKLNSTRKNEHSAKSNSSFSMFDDDIGQEA
MYTAELLNEARNFMFRLLKNVFEHGGSDLFISADFPPSMKHQGMMKPLSSQALTGEKTRLFAYSLMNDKQRDEFERELEC
NFAISIPEVSRFRINVFQQQLNVGMVIRTITSEIPTFSKLKLPASLKDVIMEKRGLILVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMVTHREVGVDCHNWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTEDGNGRRAAVEIMLNTPLVSDVILKGNFHELKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEAIRNADSMNELRLKIKLNSTRKNEHSAKSNSSFSMFDDDIGQEA
Nucleotide
Download Length: 1170 bp
>NTDB_id=428640 G8D99_RS07710 WP_166324085.1 1648318..1649487(+) (pilU) [Acinetobacter lanii strain 185]
ATGTACACAGCTGAATTATTGAATGAAGCGCGCAATTTTATGTTTCGCTTACTCAAAAATGTCTTTGAGCACGGAGGCTC
AGATCTGTTTATTTCTGCAGATTTTCCACCGAGTATGAAGCATCAAGGCATGATGAAGCCTTTAAGTTCTCAAGCCTTAA
CCGGTGAAAAAACCAGACTGTTTGCTTACAGCCTGATGAATGATAAGCAACGTGACGAATTCGAACGAGAACTTGAGTGT
AACTTTGCAATCAGCATTCCAGAAGTTTCACGCTTCCGGATCAATGTGTTTCAACAACAGCTCAATGTCGGCATGGTGAT
TCGAACCATTACATCTGAAATTCCAACCTTCAGTAAACTGAAACTTCCAGCATCGTTAAAAGATGTAATTATGGAAAAAC
GCGGTCTAATTTTGGTGGTTGGGGGAACCGGTTCAGGTAAATCGACTTCATTGGCGGCAATGATTGATCATCGTAATGAA
AACTCAGCAGGGCATATCATTACTGTTGAAGATCCTGTGGAATATGTACATAAACATAAAAAGTCGATGGTGACGCATCG
TGAAGTGGGTGTGGATTGTCACAATTGGCACAATGCACTCAAGAACACCTTACGCCAAGCACCTGACGTGATTTTGATTG
GTGAGATTCGTGATACCGAAACTATGGAACATGCCATCGCATTTGCTGAAACTGGACACCTTTGTCTAGGCACATTGCAT
GCCAATAATGCCAATCAAACCCTGGATCGCATTATTAACTTTTTCCCTGAAGAACGTCGCAACCAATTGTTAATGGATTT
ATCTTCCAATATGAAAGCGATTATTTCTCAGCGACTGATTCGTACCGAAGATGGCAATGGCCGTCGTGCCGCAGTGGAAA
TTATGCTGAATACACCTTTAGTGTCGGATGTGATTTTAAAAGGGAATTTCCATGAGCTGAAAGAGATCATGACCAAATCA
CGTGAATTGGGTATGCAGACTTTCGACCAAGCGCTTTTTGATTTATATAATGAAGGTGCCATCAGTTACGATGAGGCTAT
TCGTAATGCAGACTCTATGAATGAACTGCGTTTAAAAATCAAACTGAATAGTACCCGTAAAAATGAGCATTCAGCAAAGA
GTAATAGTTCTTTTAGTATGTTTGATGATGATATTGGGCAAGAAGCATAA
ATGTACACAGCTGAATTATTGAATGAAGCGCGCAATTTTATGTTTCGCTTACTCAAAAATGTCTTTGAGCACGGAGGCTC
AGATCTGTTTATTTCTGCAGATTTTCCACCGAGTATGAAGCATCAAGGCATGATGAAGCCTTTAAGTTCTCAAGCCTTAA
CCGGTGAAAAAACCAGACTGTTTGCTTACAGCCTGATGAATGATAAGCAACGTGACGAATTCGAACGAGAACTTGAGTGT
AACTTTGCAATCAGCATTCCAGAAGTTTCACGCTTCCGGATCAATGTGTTTCAACAACAGCTCAATGTCGGCATGGTGAT
TCGAACCATTACATCTGAAATTCCAACCTTCAGTAAACTGAAACTTCCAGCATCGTTAAAAGATGTAATTATGGAAAAAC
GCGGTCTAATTTTGGTGGTTGGGGGAACCGGTTCAGGTAAATCGACTTCATTGGCGGCAATGATTGATCATCGTAATGAA
AACTCAGCAGGGCATATCATTACTGTTGAAGATCCTGTGGAATATGTACATAAACATAAAAAGTCGATGGTGACGCATCG
TGAAGTGGGTGTGGATTGTCACAATTGGCACAATGCACTCAAGAACACCTTACGCCAAGCACCTGACGTGATTTTGATTG
GTGAGATTCGTGATACCGAAACTATGGAACATGCCATCGCATTTGCTGAAACTGGACACCTTTGTCTAGGCACATTGCAT
GCCAATAATGCCAATCAAACCCTGGATCGCATTATTAACTTTTTCCCTGAAGAACGTCGCAACCAATTGTTAATGGATTT
ATCTTCCAATATGAAAGCGATTATTTCTCAGCGACTGATTCGTACCGAAGATGGCAATGGCCGTCGTGCCGCAGTGGAAA
TTATGCTGAATACACCTTTAGTGTCGGATGTGATTTTAAAAGGGAATTTCCATGAGCTGAAAGAGATCATGACCAAATCA
CGTGAATTGGGTATGCAGACTTTCGACCAAGCGCTTTTTGATTTATATAATGAAGGTGCCATCAGTTACGATGAGGCTAT
TCGTAATGCAGACTCTATGAATGAACTGCGTTTAAAAATCAAACTGAATAGTACCCGTAAAAATGAGCATTCAGCAAAGA
GTAATAGTTCTTTTAGTATGTTTGATGATGATATTGGGCAAGAAGCATAA
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilU | Pseudomonas stutzeri DSM 10701 |
57.219 |
96.144 |
0.55 |
| pilU | Acinetobacter baylyi ADP1 |
56.609 |
89.46 |
0.506 |
| pilU | Vibrio cholerae strain A1552 |
48.459 |
91.774 |
0.445 |
| pilT | Acinetobacter nosocomialis M2 |
42.727 |
84.833 |
0.362 |
| pilT | Acinetobacter baumannii D1279779 |
42.727 |
84.833 |
0.362 |
| pilT | Acinetobacter baumannii strain A118 |
42.727 |
84.833 |
0.362 |