Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G7047_RS17120 Genome accession   NZ_CP049910
Coordinates   3748057..3749793 (+) Length   578 a.a.
NCBI ID   WP_166307973.1    Uniprot ID   A0A6G8CRK3
Organism   Diaphorobacter sp. HDW4A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3743057..3754793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7047_RS17095 (G7047_17100) cgtA 3744181..3745311 (-) 1131 WP_166307964.1 Obg family GTPase CgtA -
  G7047_RS17100 (G7047_17105) rpmA 3745670..3745927 (-) 258 WP_166307967.1 50S ribosomal protein L27 -
  G7047_RS17105 (G7047_17110) rplU 3745939..3746250 (-) 312 WP_166307970.1 50S ribosomal protein L21 -
  G7047_RS17110 (G7047_17115) - 3746571..3747500 (+) 930 WP_166312117.1 polyprenyl synthetase family protein -
  G7047_RS17120 (G7047_17125) pilB 3748057..3749793 (+) 1737 WP_166307973.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G7047_RS17125 (G7047_17130) pilC 3749821..3751038 (+) 1218 WP_166307976.1 type II secretion system F family protein Machinery gene
  G7047_RS17130 (G7047_17135) - 3751038..3752006 (+) 969 WP_166307979.1 A24 family peptidase -
  G7047_RS17135 (G7047_17140) coaE 3752014..3752634 (+) 621 WP_166307982.1 dephospho-CoA kinase -
  G7047_RS17140 (G7047_17145) zapD 3752708..3753463 (+) 756 WP_166307985.1 cell division protein ZapD -
  G7047_RS17145 (G7047_17150) - 3753486..3753707 (+) 222 WP_166307988.1 DNA gyrase inhibitor YacG -
  G7047_RS17150 (G7047_17155) - 3753708..3754154 (-) 447 WP_166307991.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62897.40 Da        Isoelectric Point: 5.2433

>NTDB_id=428568 G7047_RS17120 WP_166307973.1 3748057..3749793(+) (pilB) [Diaphorobacter sp. HDW4A]
MAAVDTAPKSVTPIVLPGLARTLMSAGKLDQKAAEDIAKKVQGSKSAFIAELINSGIITAAELAHTVSSVFGAPLIDVDA
LDTMRLPQDALDAKICLNYRVAVLNKRGNRLIIATADPTDHEAAEQIKFTTQMGVDWIIAEYDKLGKFVEKITKGASEAL
DSIVSGGDFEFGDVPVEVAEDSQDVGQNDVEDAPIVKFLQKMLIDAFNMRASDLHFEPYEHTYRVRFRIDGELREISSPP
PAIKEKLASRIKVISRLDISEKRVPQDGRMKLKIGADRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIEALGYEPEEKA
RLLTAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPSEINLPGVNQVNVNEKAGMNFAVALKAFLRQDP
DIIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPNCKEPADIP
HEALVDAGYLEEEIDGSWVTYKPVGCSACNNGYKGRVGIYEVMPISEEMQRIILRDGSALEIAKQARLEGVRSLRTSGLY
KARTGMTSLEEVLACTNE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=428568 G7047_RS17120 WP_166307973.1 3748057..3749793(+) (pilB) [Diaphorobacter sp. HDW4A]
ATGGCCGCTGTTGATACCGCCCCCAAAAGCGTGACCCCCATCGTTCTGCCAGGACTGGCCAGAACATTGATGTCTGCTGG
GAAACTTGATCAGAAGGCCGCAGAAGACATCGCCAAGAAGGTGCAGGGCTCGAAGTCCGCCTTCATCGCGGAACTGATCA
ACTCGGGAATCATTACCGCCGCCGAGCTGGCACATACGGTTTCAAGCGTTTTCGGAGCCCCGTTGATCGATGTCGACGCA
CTGGACACCATGCGTCTGCCACAGGACGCACTCGACGCCAAAATCTGCCTGAACTATCGTGTCGCGGTACTGAACAAGCG
CGGCAATCGTCTCATCATTGCAACAGCCGACCCGACCGACCACGAGGCGGCCGAGCAGATCAAGTTCACGACCCAGATGG
GCGTCGACTGGATCATTGCTGAGTACGATAAGCTTGGCAAGTTCGTAGAGAAGATCACCAAAGGTGCCTCAGAGGCCTTG
GACAGCATCGTGTCAGGTGGAGATTTCGAGTTCGGCGACGTCCCGGTCGAAGTGGCGGAGGATTCGCAGGACGTCGGACA
GAACGACGTCGAAGACGCTCCCATCGTCAAATTTCTGCAGAAGATGCTGATCGACGCATTCAACATGCGCGCATCCGACC
TACATTTCGAACCCTATGAACACACCTACCGCGTGCGTTTTCGCATCGATGGTGAATTGCGGGAAATCTCCAGCCCACCG
CCAGCCATCAAGGAAAAGCTGGCCTCACGCATCAAGGTGATCTCGCGCCTCGATATTTCAGAAAAGCGTGTGCCACAGGA
CGGCCGAATGAAGCTCAAGATCGGGGCCGATCGTGTGATCGATTTCCGTGTGAGCACTTTGCCGACGTTGTTTGGCGAGA
AGATCGTGATCCGTATTCTGGACCCCAGCAGCGCCAAGCTCGGCATTGAGGCCCTCGGCTACGAACCCGAGGAAAAGGCA
CGCCTGCTCACAGCCATCGGCCGCCCCTACGGCATGATTCTGGTCACCGGCCCCACTGGCTCTGGCAAGACCGTGTCGCT
CTACACCTGCCTGAACCTGCTCAACAAACCCGGAGTAAACATTTCGACGGCGGAAGACCCGTCCGAAATCAACTTGCCAG
GCGTGAATCAGGTCAACGTCAATGAAAAGGCGGGGATGAACTTCGCGGTCGCACTCAAGGCGTTTCTGCGTCAGGATCCG
GACATCATCATGGTCGGTGAAATCCGTGACCTGGAAACGGCGGACATCGCCATCAAGGCCGCTCAGACCGGTCACTTGGT
GCTTTCCACGCTGCACACGAACGATGCGCCCACCACGTTGACGCGGATGCGCAACATGGGGATTGCGCCATTCAACATCG
CGTCGAGCGTGATTCTGATCACCGCTCAGCGTCTTGCTCGCCGCCTTTGCCCCAATTGCAAGGAGCCAGCCGACATCCCG
CACGAAGCGCTGGTGGACGCCGGATATCTAGAAGAAGAGATCGATGGTTCCTGGGTGACCTACAAGCCCGTGGGCTGCAG
CGCCTGCAACAACGGCTACAAGGGCCGGGTCGGCATCTACGAGGTGATGCCGATCTCGGAAGAAATGCAACGCATCATTC
TTCGCGATGGCAGCGCATTGGAGATCGCCAAACAGGCGCGCCTGGAGGGCGTGCGTTCGCTGCGCACCTCTGGCCTGTAC
AAGGCCCGAACCGGCATGACTTCGCTGGAAGAAGTTCTGGCCTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-152)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8CRK3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.901

100

0.536
  pilB Acinetobacter baylyi ADP1

52.405

100

0.528
  pilF Neisseria gonorrhoeae MS11

50.177

97.578

0.49
  pilB Legionella pneumophila strain ERS1305867

48.393

96.886

0.469
  pilB Vibrio campbellii strain DS40M4

45.583

97.924

0.446
  pilB Vibrio cholerae strain A1552

45.133

97.751

0.441
  pilB Vibrio parahaemolyticus RIMD 2210633

45.161

96.54

0.436
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.406

93.772

0.379
  pilF Thermus thermophilus HB27

40.111

93.599

0.375