Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G7048_RS05600 Genome accession   NZ_CP049905
Coordinates   1169968..1171707 (+) Length   579 a.a.
NCBI ID   WP_166067188.1    Uniprot ID   A0A6G8BTK0
Organism   Diaphorobacter sp. HDW4B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1164968..1176707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7048_RS05575 (G7048_05570) cgtA 1166098..1167240 (-) 1143 WP_166067185.1 Obg family GTPase CgtA -
  G7048_RS05580 (G7048_05575) rpmA 1167589..1167846 (-) 258 WP_166067186.1 50S ribosomal protein L27 -
  G7048_RS05585 (G7048_05580) rplU 1167860..1168171 (-) 312 WP_166067187.1 50S ribosomal protein L21 -
  G7048_RS05590 (G7048_05585) - 1168494..1169423 (+) 930 WP_166070816.1 polyprenyl synthetase family protein -
  G7048_RS05600 (G7048_05595) pilB 1169968..1171707 (+) 1740 WP_166067188.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G7048_RS05605 (G7048_05600) pilC 1171735..1172952 (+) 1218 WP_166067189.1 type II secretion system F family protein Machinery gene
  G7048_RS05610 (G7048_05605) - 1172970..1173911 (+) 942 WP_371747651.1 A24 family peptidase -
  G7048_RS05615 (G7048_05610) coaE 1174006..1174548 (+) 543 WP_371747652.1 dephospho-CoA kinase -
  G7048_RS05620 (G7048_05615) zapD 1174620..1175375 (+) 756 WP_166067192.1 cell division protein ZapD -
  G7048_RS05625 (G7048_05620) - 1175397..1175618 (+) 222 WP_166067193.1 DNA gyrase inhibitor YacG -
  G7048_RS05630 (G7048_05625) - 1175652..1176101 (-) 450 WP_166067195.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 63028.44 Da        Isoelectric Point: 5.1627

>NTDB_id=428540 G7048_RS05600 WP_166067188.1 1169968..1171707(+) (pilB) [Diaphorobacter sp. HDW4B]
MAAVDTAPKSATPIVLPGLARALMSAGKLDQKAAEDIAKKAQATRSTFIAELIGSGTITSADLAHTISSVFGAPLIDVSV
FDPLKLPKEVLDAKICMTYRVAVLSKRGNRLIIATADPTDHEAAEQIKFTTQMGVDWIIAEYDKLGKFVEQASKGASEAL
DSIVSGGGGDFEFGDVQVEEETESQDVGQDVEDAPIVKFLQKMLIDAFNMRASDLHFEPYEHTYRVRFRIDGELREISSP
PPAIKEKLASRIKVISRLDISEKRVPQDGRMKLKIGADRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIEALGYEPEEK
ERLLTAIVRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPSEINLPGVNQVNVNDKAGLTFSVALKAFLRQD
PDIIMVGEIRDLETADIAIKAAQTGHMVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPNCKEPADI
PHESLVEAGYKEEDIDGSWVTYKPVGCSACNNGYKGRVGIYEVMPISEEMQRIILRDGSALEIAQQARIEGVRSLRQSGL
YKAKIGMTSLEEVLACTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=428540 G7048_RS05600 WP_166067188.1 1169968..1171707(+) (pilB) [Diaphorobacter sp. HDW4B]
ATGGCCGCTGTAGACACTGCCCCCAAAAGCGCGACCCCCATTGTTCTGCCTGGTTTGGCACGAGCCTTGATGTCCGCTGG
CAAGCTCGATCAGAAGGCCGCAGAGGACATCGCCAAGAAGGCGCAAGCCACGCGCAGCACCTTCATCGCCGAGCTGATCG
GCTCCGGTACGATCACATCGGCTGATCTGGCACATACGATTTCCAGCGTCTTCGGCGCCCCGCTGATTGACGTTTCGGTA
TTCGATCCGCTCAAGCTGCCCAAAGAAGTGCTGGATGCCAAGATTTGCATGACCTACCGCGTGGCGGTACTCAGCAAGCG
CGGAAATCGTCTCATCATTGCAACAGCCGACCCCACGGATCACGAGGCGGCCGAACAGATCAAGTTCACCACCCAGATGG
GGGTCGACTGGATCATTGCCGAGTACGACAAGCTCGGCAAATTCGTTGAACAGGCGAGCAAAGGAGCCAGCGAAGCGCTC
GACAGCATTGTCTCGGGCGGGGGGGGTGACTTCGAGTTTGGCGATGTCCAGGTCGAAGAGGAAACAGAGTCTCAGGACGT
GGGCCAGGACGTCGAAGACGCGCCCATCGTCAAGTTCCTGCAGAAGATGCTGATCGATGCGTTCAACATGCGCGCGTCGG
ACTTGCACTTCGAGCCCTACGAGCACACCTACCGCGTGCGTTTTCGCATCGATGGCGAGTTGCGCGAAATCTCCAGCCCG
CCCCCAGCGATCAAGGAAAAGCTGGCATCGCGCATCAAGGTGATCTCGCGGCTGGACATTTCAGAAAAGCGCGTGCCGCA
GGACGGGCGAATGAAGCTCAAGATCGGTGCGGATCGTGTCATCGATTTCCGCGTGAGCACCTTGCCCACGCTGTTCGGCG
AGAAGATCGTGATCCGTATTCTCGACCCGAGCAGCGCCAAGCTCGGCATCGAAGCGCTCGGCTATGAGCCCGAGGAAAAA
GAGCGTCTGCTGACGGCGATCGTGCGCCCATACGGAATGATTCTGGTCACAGGCCCCACGGGTTCGGGCAAGACCGTGTC
GCTCTACACCTGTCTGAACCTGCTGAACAAGCCGGGCGTGAACATTTCCACGGCCGAAGATCCGTCGGAAATCAACTTGC
CCGGCGTGAATCAGGTCAATGTGAACGACAAGGCGGGGTTGACCTTTTCTGTCGCGCTCAAGGCCTTTCTGCGCCAGGAT
CCCGACATCATCATGGTCGGCGAAATCCGTGACCTCGAAACCGCCGACATCGCCATCAAGGCAGCGCAGACTGGTCACAT
GGTGCTCTCGACCCTGCACACCAACGACGCGCCCACCACGTTGACGCGTATGCGCAACATGGGCATCGCGCCGTTCAATA
TTGCATCGAGCGTGATTCTGATCACCGCTCAGCGTCTGGCCCGGCGTCTGTGCCCCAACTGCAAGGAGCCTGCCGATATT
CCGCACGAATCACTGGTGGAGGCGGGCTACAAGGAAGAGGACATCGACGGTTCGTGGGTCACCTACAAACCGGTGGGCTG
CAGCGCCTGCAACAATGGCTACAAAGGACGCGTTGGCATCTATGAGGTCATGCCCATTTCCGAGGAGATGCAACGCATCA
TCCTGCGCGATGGCAGCGCGTTGGAAATTGCCCAGCAAGCACGCATCGAAGGGGTGCGTTCTTTGCGTCAGTCCGGCCTG
TACAAGGCAAAAATAGGCATGACATCGCTGGAAGAAGTTCTGGCCTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8BTK0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.401

100

0.542
  pilB Acinetobacter baumannii D1279779

52.749

100

0.53
  pilF Neisseria gonorrhoeae MS11

51.681

97.582

0.504
  pilB Legionella pneumophila strain ERS1305867

49.204

97.582

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

44.718

98.1

0.439
  pilB Vibrio campbellii strain DS40M4

44.621

97.927

0.437
  pilB Vibrio cholerae strain A1552

46.241

91.883

0.425
  pilF Thermus thermophilus HB27

41.199

92.228

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.41

92.746

0.375