Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G7045_RS01820 Genome accession   NZ_CP049885
Coordinates   413581..415320 (-) Length   579 a.a.
NCBI ID   WP_166156496.1    Uniprot ID   A0A6G8ADW6
Organism   Acidovorax sp. HDW3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408581..420320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7045_RS01790 (G7045_01790) - 409341..409790 (+) 450 WP_166156481.1 NUDIX domain-containing protein -
  G7045_RS01795 (G7045_01795) - 409841..410056 (-) 216 WP_166156484.1 DNA gyrase inhibitor YacG -
  G7045_RS01800 (G7045_01800) zapD 410053..410808 (-) 756 WP_166160315.1 cell division protein ZapD -
  G7045_RS01805 (G7045_01805) coaE 410844..411443 (-) 600 WP_166156487.1 dephospho-CoA kinase -
  G7045_RS01810 (G7045_01810) pilD 411459..412328 (-) 870 WP_166156490.1 A24 family peptidase Machinery gene
  G7045_RS01815 (G7045_01815) pilC 412328..413545 (-) 1218 WP_166156493.1 type II secretion system F family protein Machinery gene
  G7045_RS01820 (G7045_01820) pilB 413581..415320 (-) 1740 WP_166156496.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G7045_RS01825 (G7045_01825) - 415738..416094 (+) 357 WP_166156499.1 helix-turn-helix domain-containing protein -
  G7045_RS01830 (G7045_01830) - 416087..417517 (+) 1431 WP_166156504.1 HipA domain-containing protein -
  G7045_RS01835 (G7045_01835) - 417641..417844 (-) 204 WP_240919251.1 hypothetical protein -
  G7045_RS01845 (G7045_01845) - 418109..419038 (-) 930 WP_166160316.1 polyprenyl synthetase family protein -
  G7045_RS01850 (G7045_01850) rplU 419268..419579 (+) 312 WP_166156507.1 50S ribosomal protein L21 -
  G7045_RS01855 (G7045_01855) rpmA 419592..419852 (+) 261 WP_166156510.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62794.24 Da        Isoelectric Point: 5.3401

>NTDB_id=428321 G7045_RS01820 WP_166156496.1 413581..415320(-) (pilB) [Acidovorax sp. HDW3]
MAAVDTAPKQAPAIALPGLGRALMSAGKLTQQAAEDIYKKSQLSKTSFIAELTGSGAVSASDLAYTVATMFGAPLLDLAA
IDPLRLPKELLDAKLCQSYRVLPLAKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWVIAEYDKLTKLIEATTKSVSESM
DTLTAGGDFEFGDIPVEEAQESNEAEAAGDVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHNYRVRFRIDGELREIASP
PIAIKDKLASRIKVISRLDISEKRIPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPQEK
ERLLAAINRPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINMPGVNQVNVNEKAGLTFAAALKSFLRQD
PDIIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCAQCKAPADI
PHEALLEAGYSDAEIDGSWVTYKPVGCSACNNGYKGRVGIYQVMPISEEIQRIILQDGSAPDIARQAKLEGVRSLRESGL
QKVKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=428321 G7045_RS01820 WP_166156496.1 413581..415320(-) (pilB) [Acidovorax sp. HDW3]
ATGGCAGCTGTTGATACCGCACCCAAACAAGCCCCTGCAATCGCCCTTCCCGGTCTGGGGCGGGCGCTTATGTCTGCGGG
CAAGCTGACCCAGCAAGCGGCGGAAGACATCTATAAAAAATCGCAGCTGAGCAAAACCAGCTTCATCGCCGAACTGACCG
GATCGGGCGCAGTCTCCGCCAGCGATCTGGCCTATACCGTGGCCACCATGTTTGGCGCGCCCTTGCTCGATCTGGCCGCC
ATAGACCCTCTACGCCTGCCCAAGGAGTTGCTCGACGCCAAGCTCTGCCAGTCGTACCGCGTCCTGCCCCTGGCCAAACG
CAACAACCGCCTGATCGTTGCTACCGCCGACCCGACAGACCAAGAGGCGGCAGAAAAAATCAAATTCACCACCCAAATGG
GGGTGGACTGGGTCATTGCCGAGTACGACAAGCTCACCAAGCTAATCGAGGCCACCACAAAGAGCGTCAGCGAATCCATG
GATACGCTGACCGCCGGGGGCGATTTCGAGTTTGGCGACATCCCGGTCGAGGAAGCCCAGGAAAGCAACGAGGCCGAGGC
TGCCGGCGACGTGGAAGACGCCCCGGTCGTCAAATTTCTGCACAAGATGCTGCTCGATGCGTTCAACATGCGCGCCTCCG
ACTTGCACTTCGAGCCCTACGAGCACAATTACCGCGTGCGCTTTCGTATCGACGGCGAGCTGCGCGAAATCGCCTCACCG
CCGATTGCCATCAAAGACAAGCTGGCATCTCGCATCAAGGTGATCTCACGCCTGGACATTTCTGAAAAGCGCATCCCGCA
AGACGGGCGCATGAAGCTCAAGGTGGGCCCCGACCGCGTCATCGACTTCCGCGTCAGCACGCTGCCGACCTTGTTTGGCG
AAAAAATCGTGATCCGTATTCTCGATCCGAGCAGCGCCAAACTGGGGATTGATGCACTGGGCTACGAGCCACAGGAAAAA
GAGCGGCTGCTCGCCGCTATCAACCGGCCCTACGGCATGGTGCTGGTGACTGGGCCAACGGGGTCGGGCAAAACGGTGTC
GCTCTACACCTGCTTGAACCTGCTCAACAAGCCGGGGGTGAACATCGCCACCGCCGAAGACCCGTCGGAAATCAACATGC
CGGGGGTCAACCAGGTCAACGTCAACGAAAAAGCCGGCCTGACCTTTGCCGCCGCGCTCAAATCCTTCCTGCGCCAAGAT
CCTGACATCATCATGGTGGGCGAAATCCGCGACCTAGAAACCGCCGACATCGCCATCAAAGCCGCCCAAACCGGCCACTT
GGTGCTCTCCACGCTGCACACCAACGATGCTCCGACCACACTGACGCGGATGCGCAACATGGGCATTGCCCCATTCAACA
TTGCATCGAGCGTGATCCTGATTACCGCACAACGCCTGGCCCGGCGCCTGTGCGCACAGTGCAAGGCACCCGCAGACATT
CCGCACGAAGCCCTGCTCGAAGCCGGCTACAGCGACGCTGAAATCGACGGCTCCTGGGTCACCTACAAACCCGTAGGCTG
TTCTGCTTGCAACAATGGTTACAAAGGCCGCGTTGGCATTTACCAGGTGATGCCAATTTCTGAAGAAATTCAACGCATCA
TCCTGCAGGACGGCAGCGCCCCCGACATCGCCAGACAAGCCAAGCTCGAAGGCGTGCGTTCTTTGCGCGAATCGGGGCTG
CAAAAAGTCAAGCTCGGACTGACATCGCTTGAAGAAGTGCTCGCCGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-151)

Type II/IV secretion system protein (T2SSE)

(202-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8ADW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.859

100

0.542
  pilB Acinetobacter baylyi ADP1

53.701

100

0.539
  pilF Neisseria gonorrhoeae MS11

53.71

97.755

0.525
  pilB Legionella pneumophila strain ERS1305867

50.707

97.755

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

45.796

96.546

0.442
  pilB Vibrio campbellii strain DS40M4

45.07

98.1

0.442
  pilB Vibrio cholerae strain A1552

48.362

89.637

0.434
  pilF Thermus thermophilus HB27

40.246

98.273

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.161

98.791

0.387