Detailed information NTG0428080

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: G9H43_RS20220
• Genome accession: NZ_CP049830
• Coordinates: 4265009..4266715 (+)
• Length: 568 a.a.
• NCBI ID: WP_088814128.1
• Uniprot ID: -
• Organism: Aeromonas salmonicida subsp. masoucida strain RZ6S-1
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	4262532..4264851	4265009..4266715	flank	158

Gene organization within MGE regions

Location: 4262532..4266715

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G9H43_RS20200 (G9H43_20275)	-	4262532..4263449 (+)	918	WP_076611341.1	IS5-like element ISAs4 family transposase	-
G9H43_RS20205 (G9H43_20280)	-	4263506..4263889 (+)	384	WP_088814130.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
G9H43_RS20210 (G9H43_20285)	-	4263934..4264851 (+)	918	WP_088814129.1	IS5-like element ISAs4 family transposase	-
G9H43_RS20220 (G9H43_20295)	pilB	4265009..4266715 (+)	1707	WP_088814128.1	PilB family type IVa pilus assembly ATPase TapB	Machinery gene

Sequence

Protein

• Length: 568 a.a.
• Molecular weight: 62562.09 Da
• Isoelectric Point: 5.4384

>NTDB_id=428080 G9H43_RS20220 WP_088814128.1 4265009..4266715(+) (pilB) [Aeromonas salmonicida subsp. masoucida strain RZ6S-1]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLMESELDALGMEEIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNITEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide

• Length: 1707 bp

>NTDB_id=428080 G9H43_RS20220 WP_088814128.1 4265009..4266715(+) (pilB) [Aeromonas salmonicida subsp. masoucida strain RZ6S-1]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
CCTGAGCCAAGCCAAGGCCCAGCGCAAACCCTTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTTTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAAAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCAGA
CCCGACCAACGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTCCATACCGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGATGGAAAGCGAGCTGGATGCCCTCGGCATGGAGGAGATCGACGAGTCGGAGATC
TCCGAGCTGGAAGTCTCGGACGAAAACTCCCGCCTCGATGAGAGCGTCAATACCGCCGATGACGATGCCCCCATCGTCAA
ATACATCAATAAAATCATGATGGATGCCATCAAGCGCGGCGCCTCGGATCTGCACTTCGAACCCTATGAGACCAAGTACC
GTATCCGCTTTCGTATAGACGGCATACTGCACGAAATAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTTTCGGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCAGAGCGACGGCTGCCCCAGGATGGTCGCATCAAGTTAAAGCTGTCGCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCG
CAGCGCGGCTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAGGCACAGTATCTGCGTGCCCTCTCCAAGCCCCAG
GGCATGATACTGGTGACGGGCCCGACCGGCTCTGGCAAGACGGTCTCTCTCTATACCGGCCTCAATATTCTCAACATCAC
AGAGGTGAACATCTCAACCGCCGAAGATCCGGTGGAGATCAACCTGCCAGGCGTCAACCAGGTACAAGTCAATCCCAAGG
CGGGGTTGACCTTCGCCAGCGCCCTGCGATCCTTTCTTCGCCAGGATCCCGACGTTGTGATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCAGC
CGAAACCCTGACCCGGATGATGAACATGGGGGTCCCGGCCTTCAATATCGCCTCTTCGGTGACCCTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGATCACTGCAAGGCACCGGAAGTGGTGCCCGAAGCTGAATTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAGCCAGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATTATGCAAGGAGCCAACTCCCTGCAAATTGCAG
CGATTGCTCAAAAAGAGGGGATGCGAACCCTGCGTGTCTCGGGCTTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAGATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (42-147)

• Type II/IV secretion system protein (T2SSE) (191-458)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	55.712	100	0.558
pilB	Acinetobacter baylyi ADP1	55.439	100	0.556
pilB	Legionella pneumophila strain ERS1305867	54.24	99.648	0.54
pilB	Vibrio cholerae strain A1552	53.805	99.472	0.535
pilB	Vibrio campbellii strain DS40M4	52.12	99.648	0.519
pilB	Vibrio parahaemolyticus RIMD 2210633	51.601	98.944	0.511
pilF	Neisseria gonorrhoeae MS11	46.454	99.296	0.461
pilF	Thermus thermophilus HB27	40.152	92.958	0.373
pilB/pilB1	Synechocystis sp. PCC 6803	38.336	97.359	0.373