Detailed information NTG0427991

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: G8E09_RS14515
• Genome accession: NZ_CP049806
• Coordinates: 3070935..3072053 (+)
• Length: 372 a.a.
• NCBI ID: WP_017398880.1
• Uniprot ID: A0A6H0FWV9
• Organism: Acinetobacter pittii strain A1254
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3065935..3077053

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G8E09_RS14505 (G8E09_14495)	yggS	3069059..3069751 (-)	693	WP_167564033.1	YggS family pyridoxal phosphate-dependent enzyme	-
G8E09_RS14510 (G8E09_14500)	pilT	3069878..3070915 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
G8E09_RS14515 (G8E09_14505)	pilU	3070935..3072053 (+)	1119	WP_017398880.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
G8E09_RS14520 (G8E09_14510)	fur	3072123..3072560 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
G8E09_RS14525 (G8E09_14515)	omlA	3072672..3073070 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
G8E09_RS14530 (G8E09_14520)	-	3073135..3073449 (-)	315	WP_017481335.1	RnfH family protein	-
G8E09_RS14535 (G8E09_14525)	-	3073449..3074516 (-)	1068	WP_167564034.1	hypothetical protein	-
G8E09_RS14540 (G8E09_14530)	-	3074670..3075125 (-)	456	WP_002121698.1	bacteriohemerythrin	-
G8E09_RS14545 (G8E09_14535)	-	3075377..3076225 (-)	849	WP_057062207.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41917.12 Da
• Isoelectric Point: 6.6404

>NTDB_id=427991 G8E09_RS14515 WP_017398880.1 3070935..3072053(+) (pilU) [Acinetobacter pittii strain A1254]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=427991 G8E09_RS14515 WP_017398880.1 3070935..3072053(+) (pilU) [Acinetobacter pittii strain A1254]
ATGGATTTTAACGACCTACTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
AATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGGCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCCATTATGAACCGCGATAAAAGTGCG
CGCTTTCGTGTAAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTACTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGACGACTTACAATTACCGCCTGTGCTTAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTTGA
AATTGCTTTAAAGAATACTTTGCGACAAGCACCCGATGTGATCTTAATTGGTGAGATTCGCTCTCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACGGGTCACTTGGTATTAGCCACAATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAGAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCTCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A6H0FWV9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.733	99.731	0.685
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363