Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   G8E00_RS01575 Genome accession   NZ_CP049801
Coordinates   341247..341975 (+) Length   242 a.a.
NCBI ID   WP_166221535.1    Uniprot ID   A0A6G8RRZ1
Organism   Acinetobacter shaoyimingii strain 323-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 336247..346975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8E00_RS01560 (G8E00_01560) ponA 336860..339394 (-) 2535 WP_166008721.1 penicillin-binding protein PBP1a -
  G8E00_RS01565 (G8E00_01565) comM 339554..340612 (+) 1059 WP_166008720.1 pilus assembly protein PilM Machinery gene
  G8E00_RS01570 (G8E00_01570) pilN 340612..341250 (+) 639 WP_166221533.1 PilN domain-containing protein Machinery gene
  G8E00_RS01575 (G8E00_01575) pilO 341247..341975 (+) 729 WP_166221535.1 type 4a pilus biogenesis protein PilO Machinery gene
  G8E00_RS01580 (G8E00_01580) comL 341975..342502 (+) 528 WP_166221537.1 pilus assembly protein PilP Machinery gene
  G8E00_RS01585 (G8E00_01585) pilQ 342523..344688 (+) 2166 WP_166221539.1 type IV pilus secretin PilQ Machinery gene
  G8E00_RS01590 (G8E00_01590) aroK 344725..345267 (+) 543 WP_166221541.1 shikimate kinase AroK -
  G8E00_RS01595 (G8E00_01595) aroB 345342..346424 (+) 1083 WP_166221543.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 242 a.a.        Molecular weight: 27488.39 Da        Isoelectric Point: 5.8839

>NTDB_id=427898 G8E00_RS01575 WP_166221535.1 341247..341975(+) (pilO) [Acinetobacter shaoyimingii strain 323-1]
MSQDDLDNLDLDKQAAKNKQMTVERFVQQFNTLDPNNMGSWPLSVKITFWIIIFVVVCLLGYFLAIKPKLDSIDQAKAQQ
QSLLEDFKKKDSDLRNLKLYQAQLNQMEENFNQQLQQLPKETEIPSLVEDINLTGVNSGLKFKNIKLESEVAQEFFIEQP
ISIEATGDYHSFGNFVSGIAALARIVTLHDFNISAELQKDKKSDIPKIDYLIQAKTYRYVAGNAQPKDSKQPPAKKKSGG
EK

Nucleotide


Download         Length: 729 bp        

>NTDB_id=427898 G8E00_RS01575 WP_166221535.1 341247..341975(+) (pilO) [Acinetobacter shaoyimingii strain 323-1]
ATGAGTCAGGATGATTTAGACAATCTAGACTTGGACAAACAAGCAGCCAAAAATAAACAAATGACGGTTGAGCGCTTTGT
TCAACAATTTAATACACTAGATCCTAATAATATGGGTAGTTGGCCTTTATCAGTCAAAATTACCTTCTGGATTATTATTT
TTGTTGTTGTGTGCCTACTCGGATATTTTTTGGCTATTAAACCAAAACTAGATTCAATTGATCAGGCAAAAGCCCAGCAA
CAAAGCCTATTAGAAGACTTTAAAAAGAAGGATTCTGATCTTCGTAATCTTAAGCTTTATCAAGCTCAACTCAATCAAAT
GGAAGAGAATTTTAATCAGCAACTGCAACAATTACCGAAAGAAACAGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CTGGCGTGAATTCAGGATTGAAATTCAAAAATATCAAGCTAGAATCGGAAGTTGCTCAGGAGTTTTTTATAGAACAACCT
ATTTCGATTGAAGCTACTGGTGATTATCACTCTTTTGGTAATTTTGTTAGTGGTATAGCTGCACTAGCTCGAATTGTGAC
TTTACATGATTTTAATATTAGTGCTGAGCTACAAAAGGACAAAAAATCTGATATTCCAAAAATTGATTATTTAATTCAAG
CAAAGACATATCGTTATGTTGCTGGTAATGCTCAGCCAAAAGATAGTAAGCAACCACCTGCGAAAAAGAAATCGGGGGGT
GAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8RRZ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

63.866

98.347

0.628
  comO Acinetobacter baylyi ADP1

61.475

100

0.62