Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G5571_RS16815 Genome accession   NZ_CP049314
Coordinates   3529624..3531336 (+) Length   570 a.a.
NCBI ID   WP_001274994.1    Uniprot ID   A0A0J1A7N4
Organism   Acinetobacter baumannii strain Ex003     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3524624..3536336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5571_RS16780 (G5571_03346) nusA 3525509..3526993 (-) 1485 WP_000532248.1 transcription termination factor NusA -
  G5571_RS16785 (G5571_03347) rimP 3527031..3527555 (-) 525 WP_000777730.1 ribosome maturation factor RimP -
  G5571_RS16805 (G5571_03351) secG 3528197..3528526 (-) 330 WP_000555570.1 preprotein translocase subunit SecG -
  G5571_RS16810 (G5571_03352) tpiA 3528539..3529333 (-) 795 WP_000016940.1 triose-phosphate isomerase -
  G5571_RS16815 (G5571_03353) pilB 3529624..3531336 (+) 1713 WP_001274994.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G5571_RS16820 (G5571_03354) pilC 3531366..3532592 (+) 1227 WP_000279217.1 type II secretion system F family protein Machinery gene
  G5571_RS18945 (G5571_03355) pilD 3532592..3533452 (+) 861 WP_001152277.1 prepilin peptidase Machinery gene
  G5571_RS16830 (G5571_03356) coaE 3533454..3534050 (+) 597 WP_000874125.1 dephospho-CoA kinase -
  G5571_RS16835 (G5571_03357) - 3534047..3534961 (-) 915 WP_000075018.1 DMT family transporter -
  G5571_RS16840 (G5571_03358) rlmB 3534997..3535746 (-) 750 WP_001118149.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  G5571_RS16845 (G5571_03359) - 3535848..3536174 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63413.75 Da        Isoelectric Point: 5.2371

>NTDB_id=426029 G5571_RS16815 WP_001274994.1 3529624..3531336(+) (pilB) [Acinetobacter baumannii strain Ex003]
MSALHTSPKFTGFFRRLVEEKHVSAATMQTALDAAKRAKQDTVTYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PRDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVNLDGPTAQDDEEEAPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=426029 G5571_RS16815 WP_001274994.1 3529624..3531336(+) (pilB) [Acinetobacter baumannii strain Ex003]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGACGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAACTGCATTAGATGCAGCGAAAAGAGCTAAGCAGGACACAGTCACATATTTAATTGAAGAGGTTCATCTCTCCC
CTTCGTTATTGGCTGAAACAATTTCTGCTGAGTTTGCTGAACCATATTTCGATCTTGATGTTTATGACACCTCTCAGATT
CCTAGAGATCTGGTCGATCAGAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGGCAAATTTTATATGT
TGCGACCAGTAATCCAAGCAATATCGAAGCGATTGATGCAATTCGTTTTAATAGTAAATTGCTGGTTGAGCCTGTAATTG
TGGAGCACCATAAACTCGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTCAATGATGAAGAGTTT
GATCTCGATGTTAATCTTGATGGTCCTACCGCTCAAGACGATGAAGAAGAAGCTCCACAAGGCGATGAAGCCCCGATTGT
TAAATATATTAATAAGTTATTAATTGATGCCATTCGTATGGGTGCTTCAGACTTACATTTTGAACCGTACGAAAAATCCT
ATCGGGTGCGCTATCGTGTCGATGGAGTGTTACGTCAAATTGCCAATCCCCCTCTACAGCTTGCAAACCGCTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGTGTCCCACAAGATGGTCGTATCAAGCTCAAGCTGTCAAA
AACCAAAGCGATTGATTTTCGTGTGAACTCCCTCCCGACTTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGAATTGATGCACTAGGATATGAAGAAGATCAAAAAGCTTTGTTTATGGAGGCATTAGATAAACCT
CAGGGCATGCTTTTAATTACCGGACCAACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTTTAAATATTTTAAATAC
TGAAAGCTCCAATATTTCTACTGCCGAAGACCCGGTAGAAATTAACCTTGAAGGAATTAATCAGGTTAACGTAAACCCCA
AGGTAGGGCTTACTTTTGCGGCCGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCAGCTCAAACTGGCCACATGGTGATGTCAACACTACATACCAATAGTGC
TCCGGAAACATTGACCCGTCTACGCAATATGGGGGTTCCTTCGTTTAATATTGCAACATCAGTAAACCTAGTTATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGCAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCGCACCCAGATTTTCGGGTTTTTCAGCCCGTAGGTTGCCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCAGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTCAACCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J1A7N4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

99.123

100

0.991
  pilB Acinetobacter baylyi ADP1

79.649

100

0.796
  pilB Legionella pneumophila strain ERS1305867

50.794

99.474

0.505
  pilF Neisseria gonorrhoeae MS11

50.356

98.596

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.894

92.632

0.481
  pilB Vibrio campbellii strain DS40M4

50.674

91.053

0.461
  pilB Vibrio cholerae strain A1552

50.484

90.702

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.011

98.596

0.365