Detailed information NTG0423373

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: G3257_RS05420
• Genome accession: NZ_CP048832
• Coordinates: 1217750..1218889 (-)
• Length: 379 a.a.
• NCBI ID: WP_176374706.1
• Uniprot ID: -
• Organism: Janthinobacterium lividum strain EIF1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1212750..1223889

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G3257_RS05405 (G3257_05390)	glcF	1213734..1214972 (+)	1239	WP_176374705.1	glycolate oxidase subunit GlcF	-
G3257_RS05410 (G3257_05395)	-	1214965..1217076 (-)	2112	WP_034784833.1	EAL domain-containing protein	-
G3257_RS05415 (G3257_05400)	-	1217263..1217748 (-)	486	WP_034784838.1	glutathione peroxidase	-
G3257_RS05420 (G3257_05405)	pilU	1217750..1218889 (-)	1140	WP_176374706.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
G3257_RS05425 (G3257_05410)	pilT	1218893..1219936 (-)	1044	WP_035824693.1	type IV pilus twitching motility protein PilT	Machinery gene
G3257_RS05430 (G3257_05415)	-	1220007..1220708 (+)	702	WP_070278361.1	YggS family pyridoxal phosphate-dependent enzyme	-
G3257_RS05435 (G3257_05420)	proC	1220731..1221561 (+)	831	WP_092612864.1	pyrroline-5-carboxylate reductase	-
G3257_RS05440 (G3257_05425)	-	1221562..1222278 (-)	717	WP_176374707.1	anti-sigma factor	-
G3257_RS05445 (G3257_05430)	-	1222275..1222850 (-)	576	WP_244965389.1	sigma-70 family RNA polymerase sigma factor	-
G3257_RS05450 (G3257_05435)	-	1222925..1223482 (-)	558	WP_034784851.1	DUF3455 domain-containing protein	-

Sequence

Protein

• Length: 379 a.a.
• Molecular weight: 41079.19 Da
• Isoelectric Point: 6.9059

>NTDB_id=423373 G3257_RS05420 WP_176374706.1 1217750..1218889(-) (pilU) [Janthinobacterium lividum strain EIF1]
MQTTHEHYGAMHALLAQMRARSGSDLFITAGFPPAIKLDGKLTPLAGGALDAQQSAGYVRAVMNERQAAEFASSREANFA
ISPDGLGRFRVSAFVQMGQAGMVLRLINTAIPTLDGLGLPPILQDIVMSKRGLVIMVGATGCGKSTTLAAMVGHRNAHSH
GHIITIEDPVEFIHPHGNCIVTQREVGVDTDDWATALKNTLRQAPDVIQIGEIRDRDTMDHAIAFAETGHLCLATLHANN
ANQALDRIINFFPEERRQQLLMDLSLNLKGMISQRLIPSKEGGGRKAALEILLNSPLMSDLIFKGQVHEIKELMKKSREH
GMQTFDQALFDLHEGGAISYEDALRNADSVNDLRLTIKLKGATAPEPPPQTGATKLGLL

Nucleotide

• Length: 1140 bp

>NTDB_id=423373 G3257_RS05420 WP_176374706.1 1217750..1218889(-) (pilU) [Janthinobacterium lividum strain EIF1]
ATGCAGACGACGCACGAACACTATGGCGCCATGCACGCGCTGCTGGCGCAGATGCGCGCACGGAGCGGCTCGGACCTGTT
CATCACGGCCGGCTTTCCGCCCGCCATCAAGCTCGACGGCAAGCTGACGCCGCTGGCTGGCGGCGCGCTCGACGCGCAGC
AATCGGCCGGCTACGTGCGCGCCGTCATGAACGAACGCCAGGCGGCCGAGTTCGCGTCCAGCCGCGAAGCCAATTTCGCC
ATCAGCCCGGACGGTCTCGGACGTTTCCGCGTATCCGCCTTCGTGCAGATGGGCCAGGCAGGCATGGTCTTGCGCTTGAT
CAATACCGCCATCCCCACCCTCGATGGACTGGGTTTGCCCCCCATCCTGCAAGACATCGTGATGAGCAAGCGGGGCCTCG
TCATCATGGTGGGCGCCACGGGCTGCGGCAAGTCGACCACGCTGGCGGCCATGGTGGGCCACCGCAACGCGCACAGCCAC
GGCCACATCATCACCATCGAAGACCCCGTGGAATTCATCCATCCGCACGGCAACTGCATCGTCACCCAGCGCGAAGTGGG
CGTCGATACGGACGACTGGGCCACGGCCCTGAAAAACACCCTGCGCCAGGCGCCCGACGTGATACAGATCGGAGAAATAC
GCGACCGCGACACCATGGACCACGCCATCGCCTTTGCCGAAACGGGCCATCTGTGCCTGGCGACCTTGCACGCGAACAAC
GCCAACCAGGCGCTGGACCGCATCATCAATTTCTTCCCCGAAGAGCGGCGCCAGCAATTGCTGATGGACCTGTCGCTCAA
TTTGAAGGGCATGATTTCGCAGCGCTTGATCCCCAGCAAGGAAGGCGGCGGGCGCAAGGCCGCGCTGGAAATCCTGCTCA
ACTCACCGCTGATGAGCGACCTGATCTTCAAGGGCCAGGTGCACGAGATCAAGGAACTGATGAAAAAATCGCGCGAGCAT
GGCATGCAAACCTTCGACCAGGCCCTGTTCGACCTGCACGAAGGGGGCGCCATCAGCTATGAAGACGCGCTGCGCAATGC
GGACTCCGTCAACGACCTGCGCCTGACGATCAAACTCAAGGGCGCAACGGCGCCGGAACCGCCCCCGCAAACGGGCGCCA
CCAAACTGGGACTCCTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (88-277)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	63.173	93.14	0.588
pilU	Acinetobacter baylyi ADP1	59.942	91.557	0.549
pilU	Vibrio cholerae strain A1552	56.851	90.501	0.515
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.119	86.28	0.372
pilT	Acinetobacter baylyi ADP1	41.515	87.071	0.361