Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G3257_RS05425 Genome accession   NZ_CP048832
Coordinates   1218893..1219936 (-) Length   347 a.a.
NCBI ID   WP_035824693.1    Uniprot ID   A0A6I1HLJ0
Organism   Janthinobacterium lividum strain EIF1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1213893..1224936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3257_RS05410 (G3257_05395) - 1214965..1217076 (-) 2112 WP_034784833.1 EAL domain-containing protein -
  G3257_RS05415 (G3257_05400) - 1217263..1217748 (-) 486 WP_034784838.1 glutathione peroxidase -
  G3257_RS05420 (G3257_05405) pilU 1217750..1218889 (-) 1140 WP_176374706.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G3257_RS05425 (G3257_05410) pilT 1218893..1219936 (-) 1044 WP_035824693.1 type IV pilus twitching motility protein PilT Machinery gene
  G3257_RS05430 (G3257_05415) - 1220007..1220708 (+) 702 WP_070278361.1 YggS family pyridoxal phosphate-dependent enzyme -
  G3257_RS05435 (G3257_05420) proC 1220731..1221561 (+) 831 WP_092612864.1 pyrroline-5-carboxylate reductase -
  G3257_RS05440 (G3257_05425) - 1221562..1222278 (-) 717 WP_176374707.1 anti-sigma factor -
  G3257_RS05445 (G3257_05430) - 1222275..1222850 (-) 576 WP_244965389.1 sigma-70 family RNA polymerase sigma factor -
  G3257_RS05450 (G3257_05435) - 1222925..1223482 (-) 558 WP_034784851.1 DUF3455 domain-containing protein -
  G3257_RS05455 (G3257_05440) - 1223697..1224044 (-) 348 WP_092612873.1 hypothetical protein -
  G3257_RS05460 (G3257_05445) - 1224041..1224427 (-) 387 WP_176374708.1 phage holin family protein -
  G3257_RS05465 (G3257_05450) - 1224495..1224851 (-) 357 WP_034784857.1 DUF883 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38045.54 Da        Isoelectric Point: 6.6380

>NTDB_id=423374 G3257_RS05425 WP_035824693.1 1218893..1219936(-) (pilT) [Janthinobacterium lividum strain EIF1]
MDISELLAFSVSNKASDLHLSSGLPPMIRVNGDVRRLNVAPLEHKEVHSMIYDIMNDSQRKAYEEALECDFSFEIPGLAR
FRVNAYNQERGASAVLRTIPSKVLTLEELNAPRIFGELAMRPRGLVLVTGPTGSGKSTTLAAMVNHVNERLNHHILTIED
PIEFVHEPKKCLINQREVGSHTHSFSNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKSIDRI
IDVFPAEEKEMVRAMLSESLQAVISQNLLKTKDGAGRVAAHEIMLATPAVRNLIREAKVAQMYSAIQTGSNVGMQTLDQC
LSDLVRRGTISAETARSAAKAPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=423374 G3257_RS05425 WP_035824693.1 1218893..1219936(-) (pilT) [Janthinobacterium lividum strain EIF1]
ATGGACATCTCCGAACTACTCGCTTTCTCCGTCAGCAACAAGGCTTCCGACTTGCACCTGTCTTCCGGCCTGCCGCCGAT
GATACGGGTCAACGGCGACGTGCGCCGCCTGAACGTGGCGCCGCTCGAGCACAAGGAAGTGCACAGCATGATCTACGACA
TCATGAACGACAGCCAGCGCAAGGCGTATGAAGAAGCGCTGGAATGCGATTTCTCGTTCGAGATTCCCGGCCTGGCCCGC
TTCCGCGTCAACGCCTACAACCAGGAACGCGGTGCCTCGGCCGTGCTGCGCACGATTCCGTCGAAGGTGCTGACGCTGGA
AGAGCTGAACGCCCCGCGCATCTTCGGCGAACTGGCCATGCGTCCGCGGGGCCTCGTGCTGGTGACGGGCCCGACGGGCT
CCGGCAAGTCGACCACCCTGGCGGCGATGGTGAACCACGTCAACGAGCGCCTGAACCACCATATATTGACCATCGAAGAC
CCGATCGAATTCGTGCATGAACCGAAGAAATGCCTGATCAACCAGCGCGAAGTGGGCTCGCACACGCATTCGTTCAGCAA
TGCGCTGCGCTCGGCCCTGCGCGAAGACCCCGACGTGATTTTGGTGGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCCGAGACGGGCCACCTCGTGTTCGGCACCCTGCACACCTCATCGGCGGCGAAATCGATCGACCGCATC
ATCGACGTCTTCCCCGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAATCCTTGCAAGCCGTCATTTCGCAGAA
CTTGCTGAAAACCAAGGATGGCGCGGGCCGCGTGGCCGCGCATGAAATCATGCTGGCCACACCGGCCGTGCGCAACCTGA
TCCGCGAAGCAAAAGTGGCGCAAATGTATTCGGCCATCCAGACGGGCAGCAACGTGGGCATGCAGACCCTGGACCAGTGC
CTTTCCGACCTGGTGCGGCGCGGCACGATCTCGGCGGAAACAGCCCGCTCGGCCGCCAAAGCCCCCGAAAACTTCCCCGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I1HLJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

71.386

97.695

0.697
  pilT Legionella pneumophila strain ERS1305867

71.386

97.695

0.697
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

97.118

0.683
  pilT Vibrio cholerae strain A1552

70.326

97.118

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

99.424

0.522
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Vibrio cholerae strain A1552

40.58

99.424

0.403

Multiple sequence alignment