Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GN234_RS22865 Genome accession   NZ_CP048810
Coordinates   5190869..5192569 (-) Length   566 a.a.
NCBI ID   WP_109756360.1    Uniprot ID   -
Organism   Pseudomonas bijieensis strain L22-9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5185869..5197569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GN234_RS22830 (GN234_22755) - 5186218..5186844 (-) 627 WP_003205682.1 DUF1780 domain-containing protein -
  GN234_RS22835 (GN234_22760) - 5186990..5187676 (+) 687 WP_116833474.1 energy-coupling factor ABC transporter permease -
  GN234_RS22840 (GN234_22765) - 5187740..5187955 (+) 216 WP_013694234.1 hypothetical protein -
  GN234_RS22845 (GN234_22770) yacG 5187952..5188158 (-) 207 WP_109756364.1 DNA gyrase inhibitor YacG -
  GN234_RS22850 (GN234_22775) coaE 5188155..5188778 (-) 624 WP_163856593.1 dephospho-CoA kinase -
  GN234_RS22855 (GN234_22780) pilD 5188778..5189647 (-) 870 WP_176689119.1 prepilin peptidase Machinery gene
  GN234_RS22860 (GN234_22785) pilC 5189649..5190866 (-) 1218 WP_109756361.1 type II secretion system F family protein Machinery gene
  GN234_RS22865 (GN234_22790) pilB 5190869..5192569 (-) 1701 WP_109756360.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GN234_RS22870 (GN234_22795) - 5192794..5193213 (+) 420 WP_134925863.1 pilin -
  GN234_RS22875 (GN234_22800) - 5193495..5193848 (+) 354 WP_109756359.1 BON domain-containing protein -
  GN234_RS22880 (GN234_22805) - 5194160..5194456 (-) 297 WP_176689120.1 DUF2845 domain-containing protein -
  GN234_RS22885 (GN234_22810) - 5194458..5197391 (-) 2934 WP_176689121.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62737.78 Da        Isoelectric Point: 5.4307

>NTDB_id=423172 GN234_RS22865 WP_109756360.1 5190869..5192569(-) (pilB) [Pseudomonas bijieensis strain L22-9]
MNDIALSGLTKQLVLAELLTEQSAQQAYQQAQRSRIPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKETQPTGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDDTKQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPIQLANRIAARL
KVMASLDISERRKPQDGRLKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCNHCKKAIEIPEETLLKEGFPR
ERIGTFTIYEPVGCEQCNHGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=423172 GN234_RS22865 WP_109756360.1 5190869..5192569(-) (pilB) [Pseudomonas bijieensis strain L22-9]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTACTGGCCGAACTGCTCACCGAGCAAAGCGCGCAACAGGC
GTATCAACAGGCCCAGCGCAGTCGCATACCCCTGGTCAGTTATCTGGTACAGAACAAACTGGTCCAGAGCCGCCAGGTCG
CGGAAATCGCCTCGGAGCACTTCGGCGTCGCCCTACTCGACCTCAACAGCCTGGACAAGGAAACCCAGCCCACAGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCATCACGCCCTGCCGCTGTGGCGACGAGGCAACAAATTGTTCGTGGGCATCTCCGA
CCCCACCAATCACCAGGCCATCAATGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAGTTCTTCGAATCCAGTAGCACAGGCCTGGAAGGCATGGGCGATGTCGACCTCGATGGC
CTGGACATCGAATCGATCGACGATACCAAACAGGATTCCATCGCCGGCCAGGACACCGACGACGCGCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTATGAAAAAATCTACCGGG
TGCGGGTGCGCACCGACGGCATGCTGCGGGAAGTCGCCAGGCCGCCGATCCAACTGGCCAACCGTATCGCCGCGCGCCTG
AAAGTCATGGCCAGCCTCGATATTTCCGAACGACGCAAACCCCAGGACGGACGCCTGAAAATGCGCCTGTCGAAAAGCAA
ATCCATCGATTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CGCAAATGGGCATCGATGCCCTGGGCTACGAACCGGACCAGAAAGACCTGTACATGGCGGCCCTCAAGCAACCGCAAGGG
CTGATTCTGGTGACCGGGCCGACCGGCTCGGGCAAGACCGTATCGCTGTACACCGGGCTGAACATCCTCAACACCGTGGA
CATCAACATTTCCACCGCTGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTGAACCCACGCCAGG
GGCTGGACTTCGCCCAGGCCCTGCGTTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGCCTGCAGAACATGGGCATTCCAGGGTTCAACATCGCCACCGCCGTGCACCTGATCATCGCCCAGCGGC
TGGCGCGCAAGTTGTGCAACCACTGCAAGAAGGCCATCGAGATCCCCGAAGAGACCTTGCTCAAGGAAGGCTTCCCCCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACCATGGTTACAAAGGGCGCGTGGGGGT
CTACGAAGTGGTCAAGAACACGCCCGAGCTGCAGCGATTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATCTGCAAA
TGCGCAAGGACGGTTTCAATGACCTGCGTACATCGGGGCTGCTCAAAGTGATGCAGGGCGTCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.127

100

0.581
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.557
  pilB Vibrio cholerae strain A1552

50.442

99.823

0.504
  pilB Vibrio campbellii strain DS40M4

48.772

100

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.101

98.233

0.482
  pilF Neisseria gonorrhoeae MS11

48.313

99.47

0.481
  pilF Thermus thermophilus HB27

36.042

100

0.36

Multiple sequence alignment