Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G3T16_RS15730 Genome accession   NZ_CP048711
Coordinates   3539068..3540786 (-) Length   572 a.a.
NCBI ID   WP_163496057.1    Uniprot ID   A0A6C0U448
Organism   Kineobactrum salinum strain M2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3534068..3545786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3T16_RS15705 (G3T16_15705) argJ 3534674..3535899 (+) 1226 Protein_3197 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  G3T16_RS15710 (G3T16_15710) mutT 3535900..3536298 (+) 399 WP_163496053.1 8-oxo-dGTP diphosphatase MutT -
  G3T16_RS15715 (G3T16_15715) coaE 3536306..3536920 (-) 615 WP_163496054.1 dephospho-CoA kinase -
  G3T16_RS15720 (G3T16_15720) pilD 3536917..3537786 (-) 870 WP_163496055.1 A24 family peptidase Machinery gene
  G3T16_RS15725 (G3T16_15725) pilC 3537797..3539014 (-) 1218 WP_163496056.1 type II secretion system F family protein Machinery gene
  G3T16_RS15730 (G3T16_15730) pilB 3539068..3540786 (-) 1719 WP_163496057.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G3T16_RS15735 (G3T16_15735) - 3541268..3541720 (+) 453 WP_408610710.1 pilin -
  G3T16_RS15740 (G3T16_15740) - 3541868..3543781 (+) 1914 WP_163496058.1 hypothetical protein -
  G3T16_RS15745 (G3T16_15745) - 3543778..3545670 (+) 1893 WP_163496059.1 hypothetical protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62996.93 Da        Isoelectric Point: 5.0970

>NTDB_id=422460 G3T16_RS15730 WP_163496057.1 3539068..3540786(-) (pilB) [Kineobactrum salinum strain M2]
MNERAIGQLSGLAGRLVVEGLLNAEHAKEAQREAAYERVPLVKYLVDKLNLDSQRIAEVASLEFGVPLFDINAFSRASLP
IGLVDVNLVSKHHALPLFRRGNRLFIAVSDPTNLAALDEIKFHTGINTDAILVEERALSKVITEWVETHESLAEGLGDLG
NEDFENIDIGSGEERDAEENSDAVDETPIVRFINKVLIDAIKQGASDIHFEPYEKNYRVRFRTDGILREVVKPPKNLSAR
MAARLKIMSQMDISERRMPQDGRIQMKLSRNRAIDFRVNTLPTLFGEKVVLRILDPSSAQMGIDALGYEEEQKEMYLDAL
SKPQGMILVTGPTGSGKTVSLYTGINLLNQPERNISTAEDPVEINMEGVNQVHVNPKVGLNFAEALRSFLRQDPDVIMVG
EIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETVTRLLNIGVPSFNLATSVNLIIAQRLARRLCKDCSEPADDIPHSVLL
KEGFTEELLAQATIKRAVGCANCTDGYKGRVGIYEVVRITPSIAQLILAQGNAMEIHKQARAEGFNDLRTSALIKVASGV
TSLEEVNRVTTD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=422460 G3T16_RS15730 WP_163496057.1 3539068..3540786(-) (pilB) [Kineobactrum salinum strain M2]
ATGAATGAAAGGGCGATTGGGCAGCTGAGTGGCCTTGCAGGTCGCCTGGTGGTCGAAGGATTGCTCAACGCTGAGCACGC
CAAGGAGGCTCAGCGTGAGGCCGCCTATGAGCGGGTGCCGCTGGTCAAGTACCTGGTGGACAAGCTCAACCTGGACAGCC
AGCGCATCGCCGAGGTGGCTTCGCTGGAGTTCGGCGTGCCGTTGTTCGATATCAACGCCTTCAGCCGCGCCTCCCTGCCG
ATCGGGCTGGTGGACGTCAACCTGGTCAGCAAGCATCACGCCCTGCCGCTGTTCCGCCGCGGCAACCGCTTGTTCATTGC
TGTATCCGACCCCACCAACCTCGCCGCGCTGGACGAGATCAAGTTCCACACCGGCATCAACACCGATGCGATACTGGTGG
AGGAACGGGCGCTGAGCAAGGTCATCACCGAGTGGGTGGAAACCCACGAGAGCCTCGCCGAGGGGCTGGGCGACCTGGGC
AACGAGGATTTCGAGAACATCGATATTGGCAGCGGCGAGGAGCGCGACGCCGAGGAAAACTCGGATGCCGTCGACGAGAC
GCCGATTGTCCGCTTCATCAACAAGGTGCTGATCGATGCGATCAAGCAGGGCGCCTCGGACATCCACTTCGAGCCCTACG
AGAAAAACTACCGGGTGCGGTTTCGCACCGACGGCATCCTGCGGGAGGTGGTGAAGCCGCCCAAGAACCTCTCTGCGCGG
ATGGCGGCGCGCCTGAAGATCATGTCGCAGATGGACATCTCCGAGCGGCGCATGCCCCAGGACGGGCGCATCCAGATGAA
GCTGTCGCGCAACCGCGCGATCGATTTCCGGGTCAACACGCTGCCGACCCTGTTCGGGGAGAAGGTGGTGCTGCGTATCC
TCGACCCGTCCAGCGCCCAGATGGGCATCGACGCGCTGGGCTATGAGGAAGAGCAGAAGGAAATGTATCTCGACGCCCTG
AGCAAGCCCCAGGGCATGATCCTGGTCACCGGGCCCACCGGTTCGGGCAAGACCGTGTCGCTGTACACCGGCATCAATCT
GCTGAACCAGCCCGAGCGCAATATCTCCACCGCGGAAGATCCGGTGGAGATCAATATGGAAGGGGTCAACCAGGTTCACG
TCAATCCCAAGGTGGGGCTGAACTTCGCGGAGGCGTTGCGCTCCTTTCTGCGCCAGGACCCGGACGTGATCATGGTGGGG
GAGATCCGCGACCTGGAAACCGCCGAGATCGCGATCAAGGCGGCCCAGACCGGCCACCTGGTGCTGTCGACACTGCACAC
CAACAGCGCAGCGGAAACCGTTACCCGCTTGCTGAACATTGGCGTACCCTCCTTCAACCTGGCCACGTCGGTGAACCTGA
TCATCGCCCAACGGCTGGCCCGGCGCCTGTGCAAGGACTGCAGTGAACCGGCCGACGACATTCCCCACAGCGTGCTGCTG
AAGGAAGGCTTCACCGAGGAACTGCTGGCGCAGGCGACGATCAAGCGGGCCGTGGGCTGCGCCAACTGTACCGATGGCTA
CAAGGGCCGGGTGGGCATCTACGAGGTGGTGCGAATCACTCCCAGTATCGCCCAGCTGATCCTGGCACAGGGCAACGCCA
TGGAGATTCACAAACAGGCACGGGCCGAGGGCTTCAATGACCTGCGCACATCGGCGCTGATCAAGGTGGCCAGCGGCGTG
ACCAGCCTGGAAGAGGTCAACCGGGTAACCACCGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6C0U448

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.996

99.476

0.577
  pilB Acinetobacter baumannii D1279779

57.57

99.301

0.572
  pilB Legionella pneumophila strain ERS1305867

54.754

99.301

0.544
  pilB Vibrio parahaemolyticus RIMD 2210633

52.113

99.301

0.517
  pilB Vibrio campbellii strain DS40M4

51.146

99.126

0.507
  pilB Vibrio cholerae strain A1552

52.277

95.979

0.502
  pilF Neisseria gonorrhoeae MS11

47.09

99.126

0.467
  pilF Thermus thermophilus HB27

37.478

98.427

0.369

Multiple sequence alignment