Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G0030_RS12745 Genome accession   NZ_CP048681
Coordinates   2695927..2697633 (+) Length   568 a.a.
NCBI ID   WP_016659655.1    Uniprot ID   A0A6C0YJ55
Organism   Acinetobacter indicus strain Q186-3_T     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2690927..2702633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G0030_RS12705 (G0030_12705) nusA 2691593..2693077 (-) 1485 WP_005181786.1 transcription termination factor NusA -
  G0030_RS12710 (G0030_12710) rimP 2693110..2693634 (-) 525 WP_005181784.1 ribosome maturation factor RimP -
  G0030_RS12735 (G0030_12735) secG 2694504..2694833 (-) 330 WP_005181783.1 preprotein translocase subunit SecG -
  G0030_RS12740 (G0030_12740) tpiA 2694847..2695638 (-) 792 WP_005181780.1 triose-phosphate isomerase -
  G0030_RS12745 (G0030_12745) pilB 2695927..2697633 (+) 1707 WP_016659655.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G0030_RS12750 (G0030_12750) pilC 2697664..2698890 (+) 1227 WP_016659656.1 type II secretion system F family protein Machinery gene
  G0030_RS12755 (G0030_12755) pilD 2698890..2699750 (+) 861 WP_005181774.1 A24 family peptidase Machinery gene
  G0030_RS12760 (G0030_12760) coaE 2699761..2700369 (+) 609 WP_005181773.1 dephospho-CoA kinase -
  G0030_RS12765 (G0030_12765) - 2700366..2701286 (-) 921 WP_168396667.1 DMT family transporter -
  G0030_RS12770 (G0030_12770) rlmB 2701304..2702056 (-) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  G0030_RS12775 (G0030_12775) - 2702162..2702485 (-) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63758.17 Da        Isoelectric Point: 4.8722

>NTDB_id=422249 G0030_RS12745 WP_016659655.1 2695927..2697633(+) (pilB) [Acinetobacter indicus strain Q186-3_T]
MSVQQAMPRFTGFIRRLVEEGLVTAENMQQAIVAAKKAETDIVPYLIQQLQISPLTIAEKISYEFGEPIFDLAVYDSRLI
VREGLDEKLFTKYRILPIFRRHNQLYIATSNPTNIEAIDAIRFNSKLNIEVVIVEHDKLERLIEQNFTEDSTFDFDEDFD
LEVGDNAPETQEDNDEPQGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRIDGVLRQIANPPLQLATRLASRL
KVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQG
MLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCQKCKAPVEIPRQSLLELGFTE
EDLNQPEFQLYQPVGCPECREGYKGRVGVYEVMKVTPEISRIIMEDGNALQIAAASAKSGFNNLRRSGLIKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=422249 G0030_RS12745 WP_016659655.1 2695927..2697633(+) (pilB) [Acinetobacter indicus strain Q186-3_T]
ATGTCAGTACAACAAGCAATGCCTAGATTTACAGGTTTTATTCGGCGACTGGTCGAAGAAGGGCTTGTGACGGCTGAAAA
TATGCAGCAAGCCATTGTCGCGGCGAAAAAAGCCGAGACCGATATTGTGCCGTATTTAATTCAGCAGTTGCAGATTTCAC
CGCTGACCATTGCTGAAAAAATTTCCTATGAATTTGGTGAACCGATTTTTGACTTGGCGGTGTACGATTCGCGTCTGATT
GTACGTGAAGGTCTGGATGAAAAACTGTTCACCAAATACCGAATTCTGCCCATTTTCCGACGTCACAATCAGCTTTATAT
TGCTACCAGTAACCCAACCAATATCGAAGCCATTGATGCGATCCGCTTTAACAGCAAGCTGAATATTGAAGTGGTGATTG
TTGAGCATGACAAGCTGGAACGGCTGATTGAACAGAACTTTACCGAAGACAGCACCTTTGACTTTGATGAAGACTTTGAT
CTAGAAGTTGGCGACAACGCTCCCGAAACACAAGAAGATAACGATGAGCCACAAGGCGATGAAGCCCCAATCGTCAAATA
TATTAACAAGTTACTGGTCGATGCAATCCGTATGGGTGCCTCGGATTTACACTTCGAGCCTTATGAAAAAAGCTACCGGG
TCCGTTACCGGATTGACGGCGTACTGCGCCAGATTGCCAATCCTCCGTTACAGCTGGCCACACGCCTCGCCTCACGTTTA
AAAGTTATGTCACAAATGGACATCTCGGAAAAACGTATTCCGCAGGATGGCCGGATCAAGCTCAAGCTGTCAAAAAACAA
AGCCATCGACTTCCGTGTCAACTCATTGCCGACCCTGTTTGGAGAAAAAATCGTACTGCGTATCCTCGATCCATCTAGCG
CCATGCTGGGGATTGATGCACTTGGTTATGAACCGGAGCAAAAAGAGCTGTTTATGGAGGCGCTGGACAAACCACAAGGC
ATGTTGCTGATCACCGGCCCGACCGGTTCCGGTAAAACCGTATCTTTATATACCGGCTTAAACATTCTCAACCGCGAAGA
CACCAATATTTCTACCGCCGAAGATCCGGTGGAAATTAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGAAAGTGG
GTCTGACCTTTGCCGCAGCACTAAAATCATTCCTGCGCCAGGATCCAGATATTGTCATGGTCGGTGAGATCCGTGATCTG
GAAACTGCAGAAATTGCCATTAAAGCCGCACAAACCGGTCACATGGTCATGTCGACGCTGCACACCAACAGTGCACCCGA
AACCCTGACCCGTTTACGCAACATGGGCGTCCCCTCATTTAACATCGCCACCTCGGTGAACCTGGTGATTGCCCAGCGTC
TGGCACGTCGTCTCTGCCAGAAATGCAAGGCCCCGGTGGAGATTCCACGCCAGAGCCTCTTGGAACTCGGTTTTACCGAA
GAAGATTTAAACCAGCCAGAGTTCCAGCTGTATCAGCCGGTTGGCTGCCCGGAATGTCGTGAAGGTTATAAAGGCCGTGT
CGGGGTCTATGAAGTAATGAAAGTCACCCCGGAAATCTCACGCATTATTATGGAAGATGGCAATGCCTTGCAAATTGCAG
CAGCCTCGGCAAAATCCGGGTTTAACAATTTACGCCGTTCAGGATTAATCAAGGTCATGCAGGGGGTCACTTCTTTACAG
GAAGTGAATCGTGTGACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6C0YJ55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.526

100

0.808
  pilB Acinetobacter baylyi ADP1

78.07

100

0.783
  pilF Neisseria gonorrhoeae MS11

51.964

98.592

0.512
  pilB Legionella pneumophila strain ERS1305867

50.53

99.648

0.504
  pilB Vibrio cholerae strain A1552

49.912

100

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

49.553

98.415

0.488
  pilB Vibrio campbellii strain DS40M4

50.864

91.725

0.467
  pilF Thermus thermophilus HB27

37.258

100

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

33.821

100

0.366

Multiple sequence alignment