Detailed information NTG0422171

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: G0028_RS08660
• Genome accession: NZ_CP048659
• Coordinates: 1767698..1768885 (+)
• Length: 395 a.a.
• NCBI ID: WP_174492793.1
• Uniprot ID: -
• Organism: Acinetobacter piscicola strain YH12207_T
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1762698..1773885

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G0028_RS08645 (G0028_08625)	-	1763195..1763869 (+)	675	WP_180045432.1	class I SAM-dependent methyltransferase	-
G0028_RS08650 (G0028_08630)	plsB	1763925..1766093 (-)	2169	WP_180045433.1	glycerol-3-phosphate 1-O-acyltransferase PlsB	-
G0028_RS08655 (G0028_08635)	cysK	1766402..1767400 (+)	999	WP_180045434.1	cysteine synthase A	-
G0028_RS08660 (G0028_08640)	pilU	1767698..1768885 (+)	1188	WP_174492793.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
G0028_RS08665 (G0028_08645)	-	1769042..1769515 (+)	474	WP_130073702.1	hypothetical protein	-
G0028_RS08670 (G0028_08650)	-	1769637..1770416 (-)	780	WP_180045435.1	ferredoxin--NADP reductase	-
G0028_RS08675 (G0028_08655)	tsaA	1770522..1771241 (+)	720	WP_180045436.1	tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO	-
G0028_RS08680 (G0028_08660)	-	1771328..1773544 (-)	2217	WP_180045437.1	sel1 repeat family protein	-

Sequence

Protein

• Length: 395 a.a.
• Molecular weight: 44440.67 Da
• Isoelectric Point: 6.1529

>NTDB_id=422171 G0028_RS08660 WP_174492793.1 1767698..1768885(+) (pilU) [Acinetobacter piscicola strain YH12207_T]
MYTAELLQEAQRYMFHMLSKVVEFGGSDLFISADFPPSIKHQGLMKSLGQPELTAEKAKLFAYSLMNAKQRQDFETELEC
NFAISVPNVSRFRVNVFQQQLQVGMVIRTITAEIPNFTKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHTWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTENNDGRRAAVEILLNTPLISDVILKGNFHEIKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSTNELRLQIKLKATRNQQNGASTATSLSMLHEVEAADEDENAAE

Nucleotide

• Length: 1188 bp

>NTDB_id=422171 G0028_RS08660 WP_174492793.1 1767698..1768885(+) (pilU) [Acinetobacter piscicola strain YH12207_T]
ATGTATACAGCTGAATTATTGCAAGAAGCACAACGCTATATGTTTCACATGCTTAGTAAAGTGGTTGAATTTGGCGGTTC
AGATCTGTTTATTTCGGCAGATTTCCCACCCAGTATTAAGCATCAGGGCTTGATGAAATCTCTAGGGCAACCAGAGCTCA
CAGCAGAAAAAGCTAAACTTTTTGCTTATAGCCTGATGAATGCCAAACAACGTCAAGATTTTGAAACAGAACTTGAATGT
AATTTTGCCATCAGTGTACCTAACGTTTCGCGTTTTCGAGTCAATGTTTTTCAACAACAATTACAAGTCGGTATGGTGAT
TCGTACCATTACTGCTGAAATTCCAAATTTTACCAAACTGAAATTACCTGAATCACTGAAACATGTGATTATGGAAAAAC
GTGGCTTGGTCCTTGTGGTGGGTGGGACAGGTTCAGGAAAGTCGACGTCATTGGCAGCGATGATTGACTATCGTAACGAA
AATTCAGCAGGGCATATTATTACGGTTGAAGACCCTGTGGAATATGTACATAAGCATAAAAAATCGATGATTACCCATCG
TGAAGTTGGTGTAGATTGTCATACTTGGCACAATGCATTGAAAAACACTTTACGTCAGGCGCCTGATGTCATTTTAATTG
GTGAGATCCGTGATACAGAAACCATGGAACATGCCATTGCTTTTGCTGAAACAGGACATTTGTGTTTAGGTACTCTGCAT
GCAAATAATGCCAACCAAGCTTTAGACCGTATTATTAACTTTTTCCCTGAAGAGCGCCGTAATCAGCTGTTAATGGATTT
ATCATCAAATATGAAAGCCATTATTTCACAGCGTTTGATTCGCACTGAAAATAACGATGGACGTAGAGCAGCAGTTGAAA
TTTTACTCAATACACCTTTAATTTCCGATGTGATCTTAAAAGGTAATTTTCATGAAATTAAAGAAATCATGACCAAGTCG
CGTGAGCTTGGTATGCAAACTTTTGATCAGGCATTATTTGATTTATACAATGAAGGTGCGATTAGTTATGACGAAGCACT
GAGAAATGCAGATTCAACCAATGAATTACGTTTACAGATTAAGTTAAAAGCTACACGAAATCAGCAAAATGGGGCATCTA
CAGCGACCAGTTTAAGTATGTTGCATGAAGTCGAAGCAGCTGATGAAGATGAAAATGCTGCTGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (47-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.08	94.684	0.522
pilU	Acinetobacter baylyi ADP1	54.674	89.367	0.489
pilU	Vibrio cholerae strain A1552	48.656	94.177	0.458
pilT	Acinetobacter nosocomialis M2	43.865	82.532	0.362
pilT	Acinetobacter baumannii D1279779	43.865	82.532	0.362
pilT	Acinetobacter baumannii strain A118	43.865	82.532	0.362