Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   G3M63_RS02040 Genome accession   NZ_CP048629
Coordinates   434719..436887 (-) Length   722 a.a.
NCBI ID   WP_150300152.1    Uniprot ID   -
Organism   Pseudomonas sp. OIL-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 429719..441887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M63_RS02025 (G3M63_02025) - 431505..432980 (-) 1476 WP_163981739.1 AAA family ATPase -
  G3M63_RS02030 (G3M63_02030) aroB 432977..434068 (-) 1092 WP_163981740.1 3-dehydroquinate synthase -
  G3M63_RS02035 (G3M63_02035) aroK 434213..434719 (-) 507 WP_150300153.1 shikimate kinase AroK -
  G3M63_RS02040 (G3M63_02040) pilQ 434719..436887 (-) 2169 WP_150300152.1 type IV pilus secretin PilQ Machinery gene
  G3M63_RS02045 (G3M63_02045) pilP 436945..437478 (-) 534 WP_371923515.1 type 4a pilus biogenesis lipoprotein PilP -
  G3M63_RS02050 (G3M63_02050) - 437475..438095 (-) 621 WP_163981741.1 type 4a pilus biogenesis protein PilO -
  G3M63_RS02055 (G3M63_02055) - 438092..438670 (-) 579 WP_163981742.1 PilN domain-containing protein -
  G3M63_RS02060 (G3M63_02060) pilM 438670..439734 (-) 1065 WP_150300149.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 722 a.a.        Molecular weight: 77775.96 Da        Isoelectric Point: 4.5429

>NTDB_id=421960 G3M63_RS02040 WP_150300152.1 434719..436887(-) (pilQ) [Pseudomonas sp. OIL-1]
MPLTRKLSLAAALALLNPLAIAADLQNMDVASLPGERVELKLSFDEPVAAPKGYTIEQPARIALDLPGVKNKLGERSRDL
GLGNARSVTVVEAGDRTRLIINLTDLAPYATRVEGNNLYVMVGADQAPGYNAPDRAAAPSAPTTASWRAADEPAVAANSP
AAASGKAIRGLDFQRGEQGEGNVIVDLSDPSVRVDVQEQGGRVRLTFPGTRLPDELRVRLDVKDFATPVNFVNASTSGND
TTINIEPNGFYEHLVYQTDNRLIVSFKPLSKTEVEQRRAADFAYTGEKLSLNFQDIEVRSVLQLIADFTDMNLVASDTVS
GSITLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQRQIAELAPLRRELIQVNYANAADIASL
FASVTAGEGEQRGSITVDDRTNSIIALETQDKLDELRRIVTQLDIPVRQVMIEARIVEANVGFDKALGVRWGGNLRMGDR
FDLFGKDGQTGVVEDPNARGLPGDDDYIPSRNTGGFIGIPERPFNTPFVDMGVLGATSGVGIGFITNNAVLDLQLSAMES
TGNGEIISQPKVVTSDKETAKILKGSEIPYQEASSSGATTTAFAEAVLSLEVTPQITPDNQVIMEVIVTKDEPDFTNEVN
GVPTIRKNEVNAKILVADGETIVIGGVFSSETSKGQQKVPFLGDLPILGRAFRRDITSESKTELLVFLTPRIINSGAISL
AK

Nucleotide


Download         Length: 2169 bp        

>NTDB_id=421960 G3M63_RS02040 WP_150300152.1 434719..436887(-) (pilQ) [Pseudomonas sp. OIL-1]
ATGCCTCTAACCAGGAAGTTATCGCTTGCAGCAGCCCTGGCGCTGTTGAATCCTCTGGCCATCGCGGCCGACCTTCAGAA
TATGGACGTGGCCTCGCTGCCGGGTGAACGGGTCGAGCTCAAGCTCAGCTTTGACGAACCCGTTGCCGCACCGAAGGGCT
ACACCATTGAGCAACCTGCACGTATCGCTCTGGATTTGCCTGGCGTCAAGAACAAGCTGGGCGAGCGCAGCCGCGACCTC
GGCCTCGGGAACGCACGTAGTGTGACCGTGGTAGAGGCCGGTGACCGGACTCGCCTGATCATCAATCTTACCGATCTTGC
GCCTTACGCCACGCGGGTCGAAGGCAACAATCTGTACGTCATGGTCGGCGCCGATCAGGCTCCGGGTTATAACGCTCCCG
ACAGAGCCGCTGCACCCAGCGCACCGACAACCGCGAGTTGGCGTGCTGCCGACGAGCCGGCCGTGGCAGCAAATTCGCCA
GCAGCTGCTTCGGGCAAAGCCATTCGCGGCCTGGATTTTCAGCGTGGAGAGCAGGGAGAGGGTAACGTCATCGTAGACCT
GTCCGACCCGTCTGTACGAGTCGACGTGCAGGAGCAGGGCGGTCGTGTGCGGCTGACTTTCCCTGGCACTCGCCTGCCTG
ATGAACTGCGCGTACGGCTGGACGTCAAGGACTTCGCAACCCCGGTCAATTTCGTCAACGCCAGCACCAGCGGTAACGAC
ACAACCATCAATATCGAACCAAACGGTTTTTACGAGCATCTGGTATATCAGACCGATAATCGTCTGATTGTCAGCTTCAA
GCCGTTGAGCAAAACTGAGGTCGAGCAACGCCGTGCTGCCGACTTTGCCTATACAGGTGAAAAACTGTCGCTTAACTTCC
AGGACATCGAAGTGCGTTCGGTGCTGCAGTTGATTGCAGACTTCACTGACATGAACCTGGTCGCCAGTGATACCGTCTCG
GGCAGCATCACTTTACGTTTGCAGAATGTGCCCTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGGCTCGACAA
GCGCAAGGTGGGTAATGTCCTGCTGGTTGCCCCGGCCGACGAAATCGCCGCCCGCGAACGTCAGGAGCTGGAATCCCAGC
GGCAGATTGCCGAGCTGGCTCCGCTGCGTCGTGAGCTGATCCAGGTGAACTACGCCAACGCAGCTGATATCGCCAGCCTG
TTCGCGTCGGTAACCGCAGGCGAGGGCGAGCAGCGTGGCTCCATCACCGTAGATGACCGCACCAACAGCATCATTGCTCT
GGAAACCCAGGACAAGCTCGACGAGCTGCGCCGCATTGTTACTCAGCTGGATATTCCGGTTCGCCAGGTCATGATCGAGG
CGCGCATCGTTGAAGCTAACGTTGGCTTCGACAAGGCACTGGGTGTGCGCTGGGGTGGCAACCTGCGAATGGGTGATCGT
TTCGATCTGTTCGGCAAGGACGGTCAGACCGGCGTAGTGGAAGATCCCAACGCGCGTGGCTTGCCTGGTGATGACGATTA
TATCCCGTCGCGGAATACCGGCGGCTTCATCGGTATTCCGGAACGTCCGTTCAACACGCCCTTTGTGGATATGGGTGTGC
TGGGTGCGACATCAGGCGTCGGCATCGGCTTTATCACCAACAACGCCGTGCTCGATTTGCAGCTGTCGGCGATGGAAAGC
ACCGGTAATGGCGAGATTATTTCCCAGCCCAAAGTGGTCACCTCTGATAAGGAAACCGCAAAGATCCTGAAGGGTTCCGA
GATCCCGTATCAGGAAGCGAGTTCCAGTGGGGCCACCACTACCGCCTTTGCTGAAGCTGTGCTGTCTCTGGAAGTAACCC
CGCAGATCACCCCGGACAACCAGGTGATTATGGAAGTGATCGTTACCAAGGACGAGCCGGACTTCACCAACGAAGTGAAC
GGGGTGCCGACTATTCGCAAGAATGAAGTGAACGCGAAGATCCTGGTGGCCGATGGCGAAACCATTGTCATCGGTGGGGT
ATTCTCCAGCGAAACCTCCAAGGGACAACAGAAGGTTCCGTTCCTGGGCGACCTGCCGATTTTGGGCCGGGCGTTCCGTC
GTGATATAACGTCGGAAAGCAAGACCGAGCTGCTGGTATTCCTCACGCCACGCATCATCAATAGTGGAGCTATTTCCCTT
GCCAAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(386-449)

AMIN domain (AMIN)

(38-119)

Secretin and TonB N terminus short domain (STN)

(312-358)

Bacterial type II and III secretion system protein (Secretin)

(557-713)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

68.457

100

0.688
  comQ Acinetobacter baylyi ADP1

39.06

100

0.403
  pilQ Acinetobacter baumannii strain A118

37.813

100

0.398
  pilQ Acinetobacter baumannii D1279779

37.813

100

0.398
  pilQ Legionella pneumophila strain Lp02

37.058

97.922

0.363

Multiple sequence alignment