Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   G3M63_RS02060 Genome accession   NZ_CP048629
Coordinates   438670..439734 (-) Length   354 a.a.
NCBI ID   WP_150300149.1    Uniprot ID   A0A6C0TRQ4
Organism   Pseudomonas sp. OIL-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 433670..444734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M63_RS02035 (G3M63_02035) aroK 434213..434719 (-) 507 WP_150300153.1 shikimate kinase AroK -
  G3M63_RS02040 (G3M63_02040) pilQ 434719..436887 (-) 2169 WP_150300152.1 type IV pilus secretin PilQ Machinery gene
  G3M63_RS02045 (G3M63_02045) pilP 436945..437478 (-) 534 WP_371923515.1 type 4a pilus biogenesis lipoprotein PilP -
  G3M63_RS02050 (G3M63_02050) - 437475..438095 (-) 621 WP_163981741.1 type 4a pilus biogenesis protein PilO -
  G3M63_RS02055 (G3M63_02055) - 438092..438670 (-) 579 WP_163981742.1 PilN domain-containing protein -
  G3M63_RS02060 (G3M63_02060) pilM 438670..439734 (-) 1065 WP_150300149.1 pilus assembly protein PilM Machinery gene
  G3M63_RS02065 (G3M63_02065) - 439921..442320 (+) 2400 WP_163981743.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38328.76 Da        Isoelectric Point: 4.3493

>NTDB_id=421961 G3M63_RS02060 WP_150300149.1 438670..439734(-) (pilM) [Pseudomonas sp. OIL-1]
MLGLIKKKSNTLLGIDISSTTVKLLELSRSGNRYKVEAYAVEPLPPSAVVEKNIVELEGVGQALEKVLARSRTSVKQAAV
AVAGSAVITKIIEMDAGFDDDELEEQIKLEADQYIPYPLDEVAIDFEVQGVSPRNPERVEVLLAACRRENVDIREAALAL
AGIDVKVVEVEAYAMERACSLVTDQLDSGQHDLTIAVVDIGATMMTLSVLSQGRTIYTREQLFGGKQLTEEIQRRYGLSQ
EEAGLAKKQGGLPDDYETEVLGPFKDAVVQQVSRSLQFFFAGNQYNDVDYILLAGGTASIPGLDQLIQQKLGTTTLVANP
FANMTLSNKVNAVALSNDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=421961 G3M63_RS02060 WP_150300149.1 438670..439734(-) (pilM) [Pseudomonas sp. OIL-1]
GTGCTAGGGCTCATAAAAAAGAAATCGAATACGCTTCTGGGTATCGATATCAGCTCCACGACCGTGAAGCTGCTAGAACT
CAGTCGCTCCGGGAACCGCTATAAGGTCGAGGCCTACGCGGTCGAACCTCTCCCACCGAGCGCGGTGGTCGAGAAAAATA
TTGTCGAGCTCGAAGGCGTCGGGCAGGCCCTGGAAAAGGTCCTGGCGCGATCCCGTACCTCGGTAAAACAGGCCGCAGTC
GCCGTAGCAGGTTCGGCGGTGATCACCAAGATCATCGAGATGGATGCCGGTTTCGATGACGATGAGCTTGAAGAACAGAT
CAAGCTTGAAGCTGACCAATATATCCCATATCCCCTTGACGAAGTTGCGATCGATTTCGAAGTCCAGGGAGTTTCCCCGC
GAAATCCTGAGCGAGTCGAAGTGTTGCTGGCAGCCTGCCGCCGAGAAAATGTCGACATACGTGAAGCGGCTCTCGCATTG
GCTGGCATCGATGTCAAAGTAGTCGAAGTCGAAGCCTACGCTATGGAACGCGCCTGTAGCCTGGTTACCGACCAGCTGGA
TAGCGGTCAGCATGATCTCACCATTGCCGTCGTGGATATCGGCGCAACGATGATGACGCTTAGCGTGCTGAGCCAGGGTC
GCACGATTTATACCCGAGAACAGCTGTTCGGCGGCAAGCAATTGACCGAAGAGATCCAGCGTCGTTACGGCCTCTCGCAG
GAAGAGGCCGGGTTGGCCAAGAAGCAGGGCGGCCTGCCAGACGATTACGAAACCGAGGTGCTGGGTCCTTTCAAGGATGC
CGTGGTCCAGCAGGTGTCGCGCTCGCTACAGTTCTTTTTCGCGGGCAATCAGTACAACGACGTCGACTACATCCTCCTTG
CGGGGGGCACCGCATCGATTCCGGGCCTGGATCAGCTGATCCAGCAAAAGCTCGGAACCACGACGCTGGTGGCCAATCCC
TTCGCAAACATGACCCTTTCCAACAAGGTAAATGCGGTTGCGCTCAGTAATGACGCGCCAGCATTGATGATTGCCTGTGG
TCTTGCAATGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6C0TRQ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463

Multiple sequence alignment