Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GZH78_RS03125 Genome accession   NZ_CP048408
Coordinates   689832..691910 (+) Length   692 a.a.
NCBI ID   WP_039766429.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain DR397     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 684832..696910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZH78_RS03105 (GZH78_03105) pilM 687046..688110 (+) 1065 WP_039766420.1 pilus assembly protein PilM Machinery gene
  GZH78_RS03110 (GZH78_03110) - 688110..688676 (+) 567 WP_074689559.1 PilN domain-containing protein -
  GZH78_RS03115 (GZH78_03115) pilO 688673..689296 (+) 624 WP_074689561.1 type 4a pilus biogenesis protein PilO -
  GZH78_RS03120 (GZH78_03120) - 689293..689817 (+) 525 WP_074689563.1 pilus assembly protein PilP -
  GZH78_RS03125 (GZH78_03125) pilQ 689832..691910 (+) 2079 WP_039766429.1 type IV pilus secretin PilQ Machinery gene
  GZH78_RS03130 (GZH78_03130) aroK 691915..692433 (+) 519 WP_011332081.1 shikimate kinase AroK -
  GZH78_RS03135 (GZH78_03135) aroB 692586..693686 (+) 1101 WP_074689567.1 3-dehydroquinate synthase -
  GZH78_RS03140 (GZH78_03140) - 693697..695298 (+) 1602 WP_163973883.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75007.13 Da        Isoelectric Point: 5.5724

>NTDB_id=421107 GZH78_RS03125 WP_039766429.1 689832..691910(+) (pilQ) [Pseudomonas fluorescens strain DR397]
MNRIFSTLGFSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVASQLASKTRD
LGSGNARTATVVEANNRTRLIISLTQLAPYDTRVEGNTVFVVVGQGSKAAAPRTAARAPRAAPAAAPVRTFTPTARAIRG
VDFQRGTAGEGNVVIDLSDPAIAPDIQEHDGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNAGVSGDRATITVEPSGT
FDYSTYQTDNKLTVSIRPLTVDDLQKRNADRNSYNGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=421107 GZH78_RS03125 WP_039766429.1 689832..691910(+) (pilQ) [Pseudomonas fluorescens strain DR397]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGCTTTCGCCGATGGTACAGGCAGCCAATCTGAA
GGCGCTGGACGTCGCGGCACTGCCGGGCGACCGGGTCGAGCTGAAGCTGTCGTTCGACGGCCCGCCCCCGCAGCCCAAGG
GCTACACCACCGACTCGCCGGCACGGATTGCCCTCGATCTGCCGGGTGTCGCCAGTCAGCTGGCAAGCAAGACCCGTGAC
CTGGGCAGTGGCAATGCCCGTACGGCCACGGTGGTCGAGGCCAATAACCGGACGCGGCTGATCATCAGCCTGACGCAACT
GGCGCCGTACGACACCCGGGTCGAGGGCAATACGGTGTTTGTGGTGGTCGGGCAGGGCAGCAAAGCCGCTGCCCCACGTA
CGGCCGCTCGTGCACCGCGCGCAGCACCTGCGGCAGCGCCGGTCCGCACGTTTACGCCGACCGCCAGAGCCATTCGCGGC
GTGGATTTCCAGCGTGGTACGGCGGGCGAGGGCAACGTGGTCATCGACTTGTCCGACCCGGCGATCGCCCCGGACATCCA
GGAGCACGACGGCAAGATCATCCTCAATTTCGCCCGTACCCAATTGCCGGAACCGCTACGGGTGCGCCTCGACGTCAAGG
ACTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGGGTCAGCGGTGATCGGGCGACGATCACCGTCGAACCCAGCGGTACT
TTCGACTATTCCACTTACCAGACCGACAACAAGCTGACCGTCAGCATCCGGCCGTTGACCGTCGATGACCTGCAAAAACG
CAACGCCGACCGCAACAGCTATAACGGCGAAAAGCTCTCGCTCAACTTTCAGGACATCGATGTGCGTTCGGTGCTGCAAC
TGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACCGTGCAGGGCGGCATTACCTTGCGCCTGCAGAACGTG
CCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGCCTGGACAAGCGCAAGGTCGGCAACGTGTTGCTGGTGGC
GCCGGCGGATGAAATCGCTGCGCGTGAGCGGCAGGAACTGGAATCGCAGAAACAGATCGCCGAACTGGCGCCGCTGCGTC
GTGAGCTGTTGCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTCACCAGCGCCGAGGCCAAG
GTCGACGAACGGGGTTCGATCACCGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGACGA
ACTGCGGCGGATCGTGGCGCAGCTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCG
ATTACGACAAGAGTCTGGGCGTGCGCTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAACACTTCCGGGGTCAGTAAC
GGTTCCTCGACCACCATCGGCACGCCGGGCAGCACCAGTACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAATAC
CTCCGGGATCGGCATCGCGTTCATCACCGACAACGTCTTGCTCGACCTTGAACTGACCGCGATGGAGAAGACCGGCAACG
GCGAAATCGTCTCGCAGCCCAAGGTGGTCACCTCCGACAAGGAAACCGCGAAGATCCTCAAGGGCACCGAGATTCCGTAC
CAGGAAGCCAGCTCCAGCGGCGCTACGTCGGTGTCCTTCAAGGAGGCGTCGCTGTCGCTGGAAGTGACGCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAATGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATTGGGGGAGTGTTCTCAAAT
ACTCAAAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.688

100

0.743
  pilQ Acinetobacter baumannii strain A118

37.784

100

0.409
  pilQ Acinetobacter baumannii D1279779

37.784

100

0.409
  comQ Acinetobacter baylyi ADP1

40.778

100

0.409
  pilQ Legionella pneumophila strain Lp02

37.63

97.543

0.367
  pilQ Legionella pneumophila strain ERS1305867

37.593

97.254

0.366

Multiple sequence alignment