Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LCGT_RS00985 Genome accession   NC_015930
Coordinates   181896..183257 (+) Length   453 a.a.
NCBI ID   WP_080567145.1    Uniprot ID   -
Organism   Lactococcus garvieae ATCC 49156     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 176896..188257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCGT_RS00945 (LCGT_0180) - 177359..178081 (-) 723 WP_003133770.1 metal ABC transporter ATP-binding protein -
  LCGT_RS00950 (LCGT_0181) - 178103..178546 (-) 444 WP_014024192.1 zinc-dependent MarR family transcriptional regulator -
  LCGT_RS00955 (LCGT_0182) infA 178748..178966 (+) 219 WP_003133772.1 translation initiation factor IF-1 -
  LCGT_RS09970 (LCGT_0183) rpmJ 179020..179136 (+) 117 WP_014024193.1 50S ribosomal protein L36 -
  LCGT_RS00960 (LCGT_0184) rpsM 179154..179519 (+) 366 WP_003133773.1 30S ribosomal protein S13 -
  LCGT_RS00965 (LCGT_0185) rpsK 179537..179920 (+) 384 WP_003133774.1 30S ribosomal protein S11 -
  LCGT_RS00970 (LCGT_0186) - 179972..180910 (+) 939 WP_003133775.1 DNA-directed RNA polymerase subunit alpha -
  LCGT_RS00975 (LCGT_0187) rplQ 180926..181306 (+) 381 WP_003133776.1 50S ribosomal protein L17 -
  LCGT_RS00985 (LCGT_0188) radA 181896..183257 (+) 1362 WP_080567145.1 DNA repair protein RadA Machinery gene
  LCGT_RS00990 (LCGT_0189) - 183391..184467 (+) 1077 WP_014024194.1 PIN/TRAM domain-containing protein -
  LCGT_RS00995 (LCGT_0190) - 184490..185119 (+) 630 WP_014024195.1 pyridoxamine 5'-phosphate oxidase family protein -
  LCGT_RS01000 (LCGT_0191) ald 185307..186428 (+) 1122 WP_014024196.1 alanine dehydrogenase -
  LCGT_RS01005 (LCGT_0192) - 186463..186957 (-) 495 WP_014024197.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49768.92 Da        Isoelectric Point: 4.8676

>NTDB_id=42085 LCGT_RS00985 WP_080567145.1 181896..183257(+) (radA) [Lactococcus garvieae ATCC 49156]
MAKKKSTFLCQECGYKSVKKLGRCPNCGAWGSFVEEVEVQEVKNQRVSLTGEHSKPMKLDQVELFDTPRVETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDTDFYLYAETNMQSIRNEVE
RLQPDFLIVDSIQTIMTPEIQSTQGSVSQVREVTGELMQLAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
QNTFRILRAVKNRFGSTNEIGIFEMQGNGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGFLLQQQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEEPTDARECFIGEIGLTGEIR
RVTRMEQRLNEASKLGFQKVYAPKNSLAGVDIPENLEVVGVTTLSECLKKVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=42085 LCGT_RS00985 WP_080567145.1 181896..183257(+) (radA) [Lactococcus garvieae ATCC 49156]
ATAGCTAAAAAAAAATCAACATTTCTTTGCCAGGAATGTGGCTACAAATCAGTAAAAAAACTCGGACGTTGTCCCAATTG
TGGTGCTTGGGGTTCCTTTGTTGAAGAAGTAGAAGTCCAAGAGGTAAAAAATCAGAGAGTGAGTCTGACAGGCGAGCACT
CTAAGCCCATGAAGTTAGATCAGGTTGAACTTTTCGATACACCGCGTGTGGAAACTGATCTGGATGAGTTTAACCGCGTC
CTTGGAGGCGGTGTCGTTCCAGGAAGTCTTGTCCTTATTGGGGGGGATCCAGGGATTGGTAAATCTACTCTACTGCTTCA
AGTATCTACGCAACTTGCTTCAAGAGGGCGTGTACTTTATGTCAGTGGGGAGGAGTCGGCTCAACAAATTAAGCTGCGTG
CCGAGCGTTTGGGCGACATCGATACAGATTTTTACCTTTACGCCGAAACCAATATGCAAAGCATCCGAAATGAAGTTGAA
CGTCTACAGCCAGACTTTCTTATTGTAGATTCGATTCAAACTATCATGACGCCTGAAATCCAGAGTACTCAGGGTTCGGT
TAGTCAGGTGCGCGAGGTGACAGGAGAATTAATGCAGTTGGCCAAAACCAACGATATTGCGACCTTTATTGTCGGACATG
TCACTAAGGAAGGGCAACTTGCGGGGCCACGTATGTTGGAACATATGGTTGATACGGTCTTGTATTTTGAGGGAGAACGT
CAAAATACATTCCGAATTTTACGCGCGGTCAAAAACCGTTTTGGTTCAACAAATGAAATTGGGATTTTCGAGATGCAGGG
CAATGGTTTAGTGGAAGTGACCAACCCGAGTGAAGTTTTTCTCGAAGAACGTTTGGAGGGCTCTACGGGCTCTGCTATCG
TCTGTGCGTTAGAAGGTACGCGTCCGATATTGGTTGAGATTCAAGCTTTAACAACGCCAACCATGTTTGGAAATGCCAAA
CGTACAACCTCTGGTTTGGACTTTAACCGTGTGAGTTTAATCATGGCAGTACTCGAAAAGCGTGCCGGTTTTCTTTTGCA
ACAACAAGATGCCTACTTAAAATCCGCAGGTGGCGTCAAGTTGGATGAACCAGCCATTGACTTAGCAGTCGCTGTAGCTA
TTGCTTCTAGCTACAAGGAAGAGCCGACAGATGCACGCGAATGCTTTATTGGCGAAATTGGCTTGACGGGTGAAATTCGT
CGTGTTACGCGGATGGAGCAACGTCTCAATGAAGCAAGCAAACTAGGTTTTCAAAAAGTTTACGCGCCTAAGAATAGCTT
GGCAGGCGTAGACATTCCAGAAAATTTGGAAGTGGTCGGTGTGACGACTTTATCAGAATGTTTGAAAAAAGTATTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(359-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

80.353

100

0.804
  radA Streptococcus pneumoniae D39

80.353

100

0.804
  radA Streptococcus pneumoniae R6

80.353

100

0.804
  radA Streptococcus pneumoniae TIGR4

80.353

100

0.804
  radA Streptococcus mitis NCTC 12261

80.353

100

0.804
  radA Streptococcus mitis SK321

80.353

100

0.804
  radA Bacillus subtilis subsp. subtilis str. 168

61.438

100

0.623

Multiple sequence alignment