Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   GZH82_RS07250 Genome accession   NZ_CP048279
Coordinates   1557785..1558108 (+) Length   107 a.a.
NCBI ID   WP_096541451.1    Uniprot ID   -
Organism   Staphylococcus sp. MI 10-1553     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1552785..1563108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZH82_RS07220 (GZH82_07220) - 1553462..1553665 (+) 204 WP_014613816.1 YqgQ family protein -
  GZH82_RS07225 (GZH82_07225) - 1553662..1554648 (+) 987 WP_162681918.1 ROK family glucokinase -
  GZH82_RS07230 (GZH82_07230) - 1554649..1554960 (+) 312 WP_019165228.1 MTH1187 family thiamine-binding protein -
  GZH82_RS07235 (GZH82_07235) - 1554973..1555602 (+) 630 WP_162681919.1 MBL fold metallo-hydrolase -
  GZH82_RS07240 (GZH82_07240) comGA 1555762..1556736 (+) 975 WP_162681920.1 competence type IV pilus ATPase ComGA Machinery gene
  GZH82_RS07245 (GZH82_07245) comGB 1556705..1557772 (+) 1068 WP_162681921.1 competence type IV pilus assembly protein ComGB -
  GZH82_RS07250 (GZH82_07250) comGC 1557785..1558108 (+) 324 WP_096541451.1 competence type IV pilus major pilin ComGC Machinery gene
  GZH82_RS07255 (GZH82_07255) comGD 1558086..1558529 (+) 444 WP_162681922.1 competence type IV pilus minor pilin ComGD -
  GZH82_RS14440 (GZH82_07260) - 1558631..1558831 (+) 201 WP_275401855.1 hypothetical protein -
  GZH82_RS07265 (GZH82_07265) - 1558737..1559222 (+) 486 WP_162681923.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GZH82_RS07270 (GZH82_07270) - 1559386..1559913 (+) 528 WP_162681924.1 shikimate kinase -
  GZH82_RS07275 (GZH82_07275) gcvT 1560072..1561163 (+) 1092 WP_162681925.1 glycine cleavage system aminomethyltransferase GcvT -
  GZH82_RS07280 (GZH82_07280) gcvPA 1561176..1562525 (+) 1350 WP_162681926.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 107 a.a.        Molecular weight: 11987.11 Da        Isoelectric Point: 8.5012

>NTDB_id=420554 GZH82_RS07250 WP_096541451.1 1557785..1558108(+) (comGC) [Staphylococcus sp. MI 10-1553]
MIQQLKSKFNQKKGFTLIEMLLVLLIISVLLILIIPNIAKQSDHIQKTGCAAQLKMVDSQIEAYALKFNHKPNTIDDLVR
EGYIKESQKTCKSGEDITIRDGEAVAS

Nucleotide


Download         Length: 324 bp        

>NTDB_id=420554 GZH82_RS07250 WP_096541451.1 1557785..1558108(+) (comGC) [Staphylococcus sp. MI 10-1553]
ATGATTCAACAACTTAAATCAAAATTCAACCAAAAGAAAGGTTTTACATTAATTGAAATGCTACTTGTTCTGTTAATTAT
TAGTGTGTTATTGATTCTCATTATCCCTAATATCGCTAAACAGTCAGATCATATTCAAAAAACAGGCTGTGCCGCACAAT
TAAAAATGGTCGATAGTCAAATTGAAGCCTATGCTTTAAAGTTTAACCATAAACCAAATACAATTGATGACTTAGTTCGT
GAAGGCTACATTAAAGAAAGTCAAAAAACATGCAAATCAGGAGAAGATATCACAATAAGAGATGGCGAAGCAGTTGCGTC
ATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Staphylococcus aureus N315

74.737

88.785

0.664
  comGC Staphylococcus aureus MW2

74.737

88.785

0.664
  comGC Bacillus subtilis subsp. subtilis str. 168

44.565

85.981

0.383
  comYC Streptococcus suis isolate S10

53.947

71.028

0.383
  comGC/cglC Streptococcus mitis SK321

43.478

85.981

0.374
  comGC Lactococcus lactis subsp. cremoris KW2

47.561

76.636

0.364
  comGC/cglC Streptococcus pneumoniae TIGR4

42.391

85.981

0.364

Multiple sequence alignment