Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GVU25_RS09360 Genome accession   NZ_CP048014
Coordinates   1952354..1953472 (+) Length   372 a.a.
NCBI ID   WP_104852406.1    Uniprot ID   -
Organism   Acinetobacter towneri strain 205     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1947354..1958472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVU25_RS09345 (GVU25_09300) - 1949845..1950120 (+) 276 WP_004975329.1 hypothetical protein -
  GVU25_RS09350 (GVU25_09305) - 1950481..1951170 (-) 690 WP_167846204.1 YggS family pyridoxal phosphate-dependent enzyme -
  GVU25_RS09355 (GVU25_09310) pilT 1951295..1952332 (+) 1038 WP_004975327.1 type IV pilus twitching motility protein PilT Machinery gene
  GVU25_RS09360 (GVU25_09315) pilU 1952354..1953472 (+) 1119 WP_104852406.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GVU25_RS09365 (GVU25_09320) fur 1953545..1953982 (-) 438 WP_167846205.1 ferric iron uptake transcriptional regulator -
  GVU25_RS09370 (GVU25_09325) - 1954101..1954499 (+) 399 WP_004975323.1 outer membrane protein assembly factor BamE -
  GVU25_RS09375 (GVU25_09330) - 1954556..1954876 (-) 321 WP_004975319.1 RnfH family protein -
  GVU25_RS09380 (GVU25_09335) - 1954873..1955958 (-) 1086 WP_004975316.1 hypothetical protein -
  GVU25_RS09385 (GVU25_09340) - 1956105..1956560 (-) 456 WP_154320417.1 bacteriohemerythrin -
  GVU25_RS09390 (GVU25_09345) - 1956824..1957672 (-) 849 WP_096902262.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41830.11 Da        Isoelectric Point: 6.7283

>NTDB_id=419443 GVU25_RS09360 WP_104852406.1 1952354..1953472(+) (pilU) [Acinetobacter towneri strain 205]
MDFNDLLNLMIQQKASDLFITADVEPSMKIHGQIVPVAKSKLTGEIVGQLLNSIMSEKQRKEFAETRECNFAITNREKTA
RFRVSAFQQRDEPGMVLRRIETKIPTMDDLKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNLKAIVAQQLIPTIDGNSRRAAIEILINSPLIADLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLAEEGGERLLHATSNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=419443 GVU25_RS09360 WP_104852406.1 1952354..1953472(+) (pilU) [Acinetobacter towneri strain 205]
ATGGACTTTAATGATTTACTGAATTTAATGATTCAGCAAAAAGCGTCTGACTTGTTTATTACAGCCGATGTTGAACCTTC
CATGAAAATTCATGGACAAATTGTGCCTGTAGCCAAAAGTAAACTTACAGGTGAAATTGTCGGTCAGCTGCTTAACTCAA
TTATGAGTGAAAAACAGCGCAAGGAATTTGCCGAAACCCGTGAATGTAACTTTGCGATTACCAACCGTGAAAAAACAGCA
CGTTTCCGTGTCAGCGCATTCCAACAACGTGATGAGCCGGGCATGGTACTCCGTCGGATTGAAACCAAAATCCCGACCAT
GGATGACTTGAAACTGCCACCTATTCTCAAAGATTTGGCGATGACCAAACGTGGCATCATCATTTTTGTAGGTGCGACAG
GTACAGGTAAATCGACCTCTTTAGCGTCTATTATTGGTTACCGCAACCAAAATTCCAAAGGTCATATCATTACCATTGAA
GACCCGATTGAATTTGTGCATGAACATGCAGGCTGTATTATTACCCAGCGTGAAGTCGGGATTGATACCGACTCCTTTGA
AATCGCATTAAAAAATACCTTGCGTCAAGCGCCTGATGTAATTCTGATTGGTGAGATTCGTTCCCGCGAAACCATGGATT
ATGCAATTGCCTTTGCCGAAACAGGTCACTTGGTATTTGCCACCCTACACGCCAACAACGCTAACCAAGCAATTGACCGT
ATTATTCACTTCTTTGAAGCAGACCGTCATAACCAGTTGTTCATGGATTTATCGCTAAACCTGAAAGCGATTGTGGCACA
GCAGTTAATTCCAACCATTGATGGCAACTCACGTCGTGCTGCGATTGAAATTTTAATTAACTCACCACTGATTGCAGATT
TAATCCGTAAAGGTGATGTGCACGAAATTAAAGACCTGATGAAGCGTTCACGTGAGTTGGGTATGCAAACCTTTGACCAA
GCTTTATACGACCTCTATAAAGCCAAGCAAATTAGCTACAAAGATGCACTTAAACACGCCGACTCGCCAAACGATTTGCG
TCTACAAATCAAACTTGCAGAAGAAGGTGGCGAGCGCTTGTTACATGCAACTTCGAATATTACCTTCGATGGGCAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.19

100

0.774
  pilU Pseudomonas stutzeri DSM 10701

68.733

99.731

0.685
  pilU Vibrio cholerae strain A1552

56.125

94.355

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395
  pilT Legionella pneumophila strain ERS1305867

42.012

90.86

0.382
  pilT Legionella pneumophila strain Lp02

42.012

90.86

0.382
  pilT Acinetobacter baumannii strain A118

41.108

92.204

0.379
  pilT Acinetobacter nosocomialis M2

41.108

92.204

0.379
  pilT Acinetobacter baumannii D1279779

41.108

92.204

0.379
  pilT Pseudomonas aeruginosa PAK

40.462

93.011

0.376
  pilT Acinetobacter baylyi ADP1

39.942

92.204

0.368
  pilT Pseudomonas stutzeri DSM 10701

39.595

93.011

0.368
  pilT Neisseria gonorrhoeae MS11

39.706

91.398

0.363
  pilT Neisseria meningitidis 8013

39.017

93.011

0.363

Multiple sequence alignment