Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FX986_RS10255 Genome accession   NZ_CP047970
Coordinates   2440555..2441709 (+) Length   384 a.a.
NCBI ID   WP_077379436.1    Uniprot ID   -
Organism   Cobetia marina strain GPM2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2435555..2446709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX986_RS10235 - 2436588..2437166 (-) 579 WP_077379444.1 YggT family protein -
  FX986_RS10240 proC 2437336..2438178 (-) 843 WP_084209472.1 pyrroline-5-carboxylate reductase -
  FX986_RS10245 - 2438373..2439125 (-) 753 WP_191236472.1 YggS family pyridoxal phosphate-dependent enzyme -
  FX986_RS10250 pilT 2439321..2440352 (+) 1032 WP_077379438.1 type IV pilus twitching motility protein PilT Machinery gene
  FX986_RS10255 pilU 2440555..2441709 (+) 1155 WP_077379436.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FX986_RS10260 - 2441816..2442973 (-) 1158 WP_191236473.1 anhydro-N-acetylmuramic acid kinase -
  FX986_RS10265 tyrS 2443157..2444365 (+) 1209 WP_084209468.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 43034.29 Da        Isoelectric Point: 6.2727

>NTDB_id=418848 FX986_RS10255 WP_077379436.1 2440555..2441709(+) (pilU) [Cobetia marina strain GPM2]
MTPHEWLQELLQLMVSKGSSDLFISTGTPPQMKVNGRMAALGDKKLGVDQVRELVLAPMSDMQRERFEQEREANFAHSLP
GVGRFRISAFYQRSQMGMVIRRIQLSIPSLEELRLPEIIKSLSETKRGLVIFVGGTGAGKSTSLASMIQHRNQTSSGHII
CIEDPIEYIHPHQRSIVTQREVGIDTESFEVALRNTLRQAPDVIMIGEIRSRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPEERHEQVWMDLSLNLKGIVAQQLLPHKSGNGAQRVPAIEVMLRSPLIVDLIRKGAVVEIKDVMKRSQQQGM
MTFDQSLYALHQQDLITEEVALAHADSANDLRLMIKFGDSDSAQEAQLDVMNAASRFSLQDDDG

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=418848 FX986_RS10255 WP_077379436.1 2440555..2441709(+) (pilU) [Cobetia marina strain GPM2]
ATGACTCCTCATGAATGGCTGCAGGAACTCCTGCAGTTGATGGTCAGCAAGGGCAGCTCGGATCTTTTCATCTCGACGGG
GACTCCGCCGCAGATGAAGGTCAATGGGCGAATGGCGGCGCTGGGTGACAAGAAGCTCGGTGTCGATCAGGTGCGAGAAC
TGGTGCTTGCGCCGATGAGCGACATGCAGCGAGAGCGCTTCGAACAGGAGCGTGAGGCCAATTTTGCCCATAGTTTGCCG
GGGGTGGGGCGTTTCCGGATCAGCGCCTTCTATCAGCGTAGCCAGATGGGCATGGTCATCCGTCGCATCCAGCTGTCGAT
TCCCAGCCTAGAAGAACTGCGCCTGCCGGAAATCATCAAGAGTCTCTCGGAGACCAAGCGTGGTCTGGTGATCTTCGTCG
GGGGCACGGGTGCCGGCAAGTCGACCTCGCTGGCCTCGATGATCCAGCACCGCAACCAGACCTCGAGTGGCCATATCATC
TGCATCGAGGATCCGATCGAGTACATCCATCCCCATCAGCGCTCCATCGTGACCCAGCGCGAAGTGGGGATCGACACCGA
GTCCTTCGAGGTGGCGCTGCGCAACACGCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGATCCGTTCGCGTGAGA
CCATGGAGCATGCGCTGACCTTTGCGGAAACCGGTCACCTGTGTCTGGCGACCTTGCACGCCAACAATGCCAACCAGGCG
CTGGATCGCATCATCCACTTCTTCCCGGAAGAGCGTCACGAACAGGTATGGATGGACCTGTCACTGAATCTCAAGGGCAT
CGTGGCCCAGCAGCTGCTGCCTCACAAGAGTGGCAATGGCGCACAGCGAGTGCCGGCCATCGAGGTGATGCTGCGCTCGC
CGCTGATCGTCGATCTGATCCGCAAGGGGGCGGTGGTGGAGATAAAGGACGTGATGAAGCGCTCCCAGCAACAGGGCATG
ATGACCTTCGATCAATCGCTCTATGCGTTGCATCAGCAGGACCTGATCACCGAGGAGGTGGCATTGGCGCATGCCGATTC
CGCCAATGACCTGCGTCTGATGATCAAGTTCGGCGATTCCGACAGTGCTCAGGAAGCGCAACTGGATGTGATGAATGCCG
CCAGTCGCTTCTCGCTGCAGGACGACGATGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.538

98.177

0.604
  pilU Vibrio cholerae strain A1552

56.911

96.094

0.547
  pilU Acinetobacter baylyi ADP1

55.932

92.188

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.348

89.844

0.398
  pilT Neisseria meningitidis 8013

40.29

89.844

0.362

Multiple sequence alignment