Detailed information NTG0418253

Bioinformatically predicted

Overview

• Name: dprA/cilB/dalA
• Type: Machinery gene
• Locus tag: M594_RS04160
• Genome accession: NZ_CP047883
• Coordinates: 841020..841868 (+)
• Length: 282 a.a.
• NCBI ID: WP_173876053.1
• Uniprot ID: A0A6M9F961
• Organism: Streptococcus mitis strain S022-V3-A4
• Function: ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology) DNA processing Competence regulation

Genomic Context

Location: 836020..846868

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M594_RS04140 (M594_04165)	-	836144..836575 (+)	432	WP_173876050.1	arginine repressor	-
M594_RS04145 (M594_04170)	recN	836582..838249 (+)	1668	WP_173876051.1	DNA repair protein RecN	-
M594_RS04150 (M594_04175)	-	838251..838979 (+)	729	WP_173876052.1	metallophosphoesterase family protein	-
M594_RS04155 (M594_04180)	lepA	839058..840881 (+)	1824	WP_001047207.1	translation elongation factor 4	-
M594_RS04160 (M594_04185)	dprA/cilB/dalA	841020..841868 (+)	849	WP_173876053.1	DNA-processing protein DprA	Machinery gene
M594_RS04165 (M594_04190)	topA	842092..844179 (+)	2088	WP_173876054.1	type I DNA topoisomerase	-
M594_RS04170 (M594_04195)	-	844436..844795 (+)	360	WP_050083961.1	YbaN family protein	-
M594_RS04175 (M594_04200)	-	844888..845520 (+)	633	WP_173876055.1	copper homeostasis protein CutC	-
M594_RS04180 (M594_04205)	-	845564..846220 (+)	657	WP_125443158.1	MmcQ/YjbR family DNA-binding protein	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31104.81 Da
• Isoelectric Point: 5.4157

>NTDB_id=418253 M594_RS04160 WP_173876053.1 841020..841868(+) (dprA/cilB/dalA) [Streptococcus mitis strain S022-V3-A4]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLEKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMVALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEKPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLATSGQDVLAEFEF

Nucleotide

• Length: 849 bp

>NTDB_id=418253 M594_RS04160 WP_173876053.1 841020..841868(+) (dprA/cilB/dalA) [Streptococcus mitis strain S022-V3-A4]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTCCTAGAATA
CGGTGAAAATGTTGATCAGGAACTTTTGTTAGGTGATATTGCAGATATATCAGGTTGCCGAAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGGAGAAGGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATACGATGCACCTGTGCTTTTATTTTACAAGGGAAATCTTGACCTCTTGAAATTTCC
TAAAGTAGCAGTTGTGGGGAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAGGGATTGGAAA
ATGAACTGGTTATCGTCAGTGGATTAGCTAAGGGCATTGATACAGCAGCTCATATGGTAGCTCTTCAGAATGGCGGCAAA
ACCATTGCAGTGATTGGAACAGGACTAGATGTATTTTATCCAAAAGCCAATAAACGCTTGCAAGACTACATCGGTAATGA
CCATCTGGTTCTTAGTGAATATGGACCTGGCGAAAAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGCCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGCTCGGGGAGTCTCATTACCTGTGAGCGAGCAATGGAGGAA
GGGCGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGGGC
AAAATTGGCCACCAGTGGGCAGGATGTTCTTGCAGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (75-271)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (3-64)

3D structure

• AlphaFold DB: A0A6M9F961

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	98.582	100	0.986
dprA/cilB/dalA	Streptococcus pneumoniae D39	98.582	100	0.986
dprA/cilB/dalA	Streptococcus pneumoniae R6	98.582	100	0.986
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	98.582	100	0.986
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	97.872	100	0.979
dprA/cilB/dalA	Streptococcus mitis SK321	97.163	100	0.972
dprA	Streptococcus mutans UA159	62.5	99.291	0.621
dprA	Lactococcus lactis subsp. cremoris KW2	55.714	99.291	0.553
dprA	Staphylococcus aureus N315	37.456	100	0.376