Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   E3T14_RS03515 Genome accession   NZ_CP047809
Coordinates   730276..730587 (-) Length   103 a.a.
NCBI ID   WP_000472256.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UP_1591     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 725276..735587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3T14_RS03480 (E3T14_03485) gcvPA 725778..727124 (-) 1347 WP_048665334.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  E3T14_RS03485 (E3T14_03490) gcvT 727144..728235 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  E3T14_RS03490 (E3T14_03495) - 728394..728918 (-) 525 WP_001015117.1 shikimate kinase -
  E3T14_RS03495 (E3T14_03500) - 728908..729054 (-) 147 WP_001789879.1 hypothetical protein -
  E3T14_RS03500 (E3T14_03505) comGF 729151..729648 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  E3T14_RS03505 (E3T14_03510) comGE 729566..729865 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  E3T14_RS03510 (E3T14_03515) comGD 729852..730298 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  E3T14_RS03515 (E3T14_03520) comGC 730276..730587 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E3T14_RS03520 (E3T14_03525) comGB 730601..731671 (-) 1071 Protein_696 competence type IV pilus assembly protein ComGB -
  E3T14_RS03525 (E3T14_03530) comGA 731643..732617 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  E3T14_RS03530 (E3T14_03535) - 732669..733292 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E3T14_RS03535 (E3T14_03540) - 733289..733618 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E3T14_RS03540 (E3T14_03545) - 733618..734604 (-) 987 WP_000161314.1 ROK family glucokinase -
  E3T14_RS03545 (E3T14_03550) - 734601..734804 (-) 204 WP_000087561.1 YqgQ family protein -

Sequence


Protein


Download         Length: 103 a.a.        Molecular weight: 11315.36 Da        Isoelectric Point: 8.5268

>NTDB_id=417006 E3T14_RS03515 WP_000472256.1 730276..730587(-) (comGC) [Staphylococcus aureus strain UP_1591]
MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQSTGCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFI
KEAQKTCKSGETITISNGEAVAN

Nucleotide


Download         Length: 312 bp        

>NTDB_id=417006 E3T14_RS03515 WP_000472256.1 730276..730587(-) (comGC) [Staphylococcus aureus strain UP_1591]
ATGTTTAAATTTCTTAAGAAAACTCAAGCGTTTACATTGATAGAGATGCTATTAGTGTTATTAATCATCAGTTTATTATT
AATTTTAATCATTCCAAATATTGCTAAACAAACTGCTCACATACAATCAACAGGTTGTAATGCACAGGTAAAAATGGTTA
ATAGTCAAATTGAAGCGTATGCATTGAAACATAATAGAAATCCATCGTCTATTGAAGACTTAATTGCAGATGGTTTTATA
AAAGAAGCACAAAAGACATGTAAATCAGGAGAGACAATCACAATTAGTAATGGAGAAGCAGTTGCAAATTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Staphylococcus aureus MW2

100

100

1

  comGC Staphylococcus aureus N315

100

100

1

  comGC/cglC Streptococcus pneumoniae TIGR4

46.078

99.029

0.456

  comGC/cglC Streptococcus pneumoniae Rx1

46.078

99.029

0.456

  comGC/cglC Streptococcus pneumoniae D39

46.078

99.029

0.456

  comGC/cglC Streptococcus pneumoniae R6

46.078

99.029

0.456

  comGC/cglC Streptococcus mitis SK321

51.163

83.495

0.427

  comGC/cglC Streptococcus mitis NCTC 12261

51.163

83.495

0.427

  comYC Streptococcus gordonii str. Challis substr. CH1

42.857

95.146

0.408

  comYC Streptococcus suis isolate S10

50

75.728

0.379

  comGC Bacillus subtilis subsp. subtilis str. 168

41.758

88.35

0.369

  comGC Lactococcus lactis subsp. cremoris KW2

46.341

79.612

0.369


Multiple sequence alignment