Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GTW26_RS29670 Genome accession   NZ_CP047643
Coordinates   6287492..6289624 (-) Length   710 a.a.
NCBI ID   WP_003141780.1    Uniprot ID   A0A0H2ZIP5
Organism   Pseudomonas aeruginosa CI27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6282492..6294624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTW26_RS29655 (GTW26_29580) - 6284144..6285799 (-) 1656 WP_003141778.1 AAA family ATPase -
  GTW26_RS29660 (GTW26_29585) aroB 6285806..6286912 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  GTW26_RS29665 (GTW26_29590) aroK 6286962..6287480 (-) 519 WP_003095833.1 shikimate kinase AroK -
  GTW26_RS29670 (GTW26_29595) pilQ 6287492..6289624 (-) 2133 WP_003141780.1 type 4a pilus secretin PilQ Machinery gene
  GTW26_RS29675 (GTW26_29600) pilP 6289678..6290202 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP -
  GTW26_RS29680 (GTW26_29605) pilO 6290199..6290822 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO -
  GTW26_RS29685 (GTW26_29610) pilN 6290819..6291415 (-) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  GTW26_RS29690 (GTW26_29615) pilM 6291415..6292479 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 710 a.a.        Molecular weight: 77317.77 Da        Isoelectric Point: 5.3155

>NTDB_id=414830 GTW26_RS29670 WP_003141780.1 6287492..6289624(-) (pilQ) [Pseudomonas aeruginosa CI27]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASAAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SSGEEKENSRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHNGNWNS
YGKNGNMGIKDKEGYNCGPFQGQCTFPTRDNSPTPFVDMGAKDASSGIGIGFITDNIILDLQLSAMEKTGNGEVVSQPKV
VTSDKETAKILKGSEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDFQNALNGVPPINKNEVNA
KILVNDGETIVIGGVFSNTQTKAVDKVPFLGDLPYLGRLFRRDTVSDTKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2133 bp        

>NTDB_id=414830 GTW26_RS29670 WP_003141780.1 6287492..6289624(-) (pilQ) [Pseudomonas aeruginosa CI27]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCACTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAACAAACTCGGCACCAAGAATCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGTTGATCATCAACCTGACCACGCT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCGCCGCTCCGGTCAAGGCAAGCCCGGCTCCGGCTAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATCCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATTCAAGAACAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAACCGAGCGGGCTCTACGACTATCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACGGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGTGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTGCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTTACT
AGTAGTGGCGAAGAGAAAGAAAATTCTCGTGGTTCGATTACCGTAGATGACCGTACCAACAGCATCATTGCCTATCAGCC
GCAGGAGCGGCTGGACGAGCTACGCCGAATCGTTTCCCAGTTGGACATCCCGGTTCGTCAGGTGATGATCGAGGCCCGTA
TCGTCGAGGCCAACGTGGGGTACGACAAGAGTCTTGGTGTGCGTTGGGGTGGTGCCTACCACAATGGCAACTGGAACAGC
TACGGCAAGAACGGCAACATGGGCATCAAGGATAAGGAAGGTTACAACTGCGGGCCGTTCCAGGGGCAGTGTACGTTCCC
GACCAGAGATAATTCTCCGACCCCATTTGTGGATATGGGGGCCAAGGATGCTAGTTCCGGTATCGGTATTGGCTTTATCA
CCGATAACATCATTCTTGACTTGCAATTATCGGCTATGGAGAAAACCGGGAACGGCGAAGTGGTTTCGCAACCCAAGGTC
GTAACTTCTGACAAGGAAACCGCGAAGATCCTAAAGGGATCGGAGATCCCATATCAGGAGGCCAGTTCCAGTGGTGCGAC
CAGCACCTCGTTCAAGGAGGCCGCTCTTTCTCTGGAGGTGACGCCGCAGATCACTCCAGACAATCGGATCATCGTTGAGG
TGAAGGTGACCAAGGATGCTCCCGACTTCCAGAATGCTTTGAATGGAGTTCCTCCGATCAACAAGAACGAGGTTAACGCC
AAGATTCTGGTCAACGATGGTGAAACCATCGTTATCGGCGGTGTATTCAGCAACACCCAGACAAAGGCTGTCGACAAGGT
ACCCTTCCTCGGTGACCTGCCTTACTTGGGCCGTTTGTTCCGCCGCGATACGGTCTCCGATACGAAAAACGAACTTCTGG
TTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(307-353)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(545-701)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIP5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

94.818

100

0.954
  comQ Acinetobacter baylyi ADP1

42.368

98.732

0.418
  pilQ Acinetobacter baumannii strain A118

40.621

99.859

0.406
  pilQ Acinetobacter baumannii D1279779

40.621

99.859

0.406
  pilQ Legionella pneumophila strain Lp02

38.248

96.479

0.369
  pilQ Legionella pneumophila strain ERS1305867

38.248

96.479

0.369

Multiple sequence alignment