Detailed information NTG0041402

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: HMPREF0833_RS08320
• Genome accession: NC_015678
• Coordinates: 1785068..1785520 (+)
• Length: 150 a.a.
• NCBI ID: WP_003019060.1
• Uniprot ID: I1ZJT7
• Organism: Streptococcus parasanguinis ATCC 15912
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1780068..1790520

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HMPREF0833_RS08285 (HMPREF0833_11653)	-	1780405..1781508 (+)	1104	WP_013904484.1	glycosyl hydrolase family 8	-
HMPREF0833_RS08290 (HMPREF0833_11654)	-	1781734..1782126 (+)	393	WP_037617571.1	DUF1033 family protein	-
HMPREF0833_RS08295 (HMPREF0833_11655)	comYA	1782212..1783153 (+)	942	WP_013904485.1	competence type IV pilus ATPase ComGA	Machinery gene
HMPREF0833_RS08300 (HMPREF0833_11656)	comYB	1783086..1784117 (+)	1032	WP_115276785.1	competence type IV pilus assembly protein ComGB	Machinery gene
HMPREF0833_RS08305 (HMPREF0833_11657)	comYC	1784114..1784431 (+)	318	WP_003019171.1	competence type IV pilus major pilin ComGC	Machinery gene
HMPREF0833_RS08310 (HMPREF0833_11658)	comYD	1784421..1784825 (+)	405	WP_041818518.1	competence type IV pilus minor pilin ComGD	Machinery gene
HMPREF0833_RS08315 (HMPREF0833_11659)	comGE	1784791..1785078 (+)	288	WP_013904488.1	competence type IV pilus minor pilin ComGE	-
HMPREF0833_RS08320 (HMPREF0833_11660)	comGF/cglF	1785068..1785520 (+)	453	WP_003019060.1	competence type IV pilus minor pilin ComGF	Machinery gene
HMPREF0833_RS08325 (HMPREF0833_11661)	comGG	1785477..1785944 (+)	468	WP_255309063.1	competence type IV pilus minor pilin ComGG	-
HMPREF0833_RS08330 (HMPREF0833_11662)	comYH	1785975..1786928 (+)	954	WP_013904490.1	class I SAM-dependent methyltransferase	Machinery gene
HMPREF0833_RS08335 (HMPREF0833_11663)	-	1786980..1788173 (+)	1194	WP_013904491.1	acetate kinase	-
HMPREF0833_RS08340 (HMPREF0833_11664)	-	1788246..1788977 (+)	732	WP_013904492.1	CPBP family intramembrane glutamic endopeptidase	-
HMPREF0833_RS08345 (HMPREF0833_11665)	folP	1789139..1790089 (+)	951	WP_013904493.1	dihydropteroate synthase	-

Sequence

Protein

• Length: 150 a.a.
• Molecular weight: 17355.77 Da
• Isoelectric Point: 5.5711

>NTDB_id=41402 HMPREF0833_RS08320 WP_003019060.1 1785068..1785520(+) (comGF/cglF) [Streptococcus parasanguinis ATCC 15912]
MWNKEKVKAFTLLEALVALLVLSGGVLVFQGLTKLLHAELDYQAHQKQEEWILFQQQLQVELDRSHFEKVADNHIYLVQD
QKPIAIGQSKGDDIRKTDDKGRGYQPMISGVRSSQIWQEGDLLHLRLDFEEGLEREYVYHVVPAIQNEKS

Nucleotide

• Length: 453 bp

>NTDB_id=41402 HMPREF0833_RS08320 WP_003019060.1 1785068..1785520(+) (comGF/cglF) [Streptococcus parasanguinis ATCC 15912]
ATGTGGAATAAAGAAAAGGTCAAGGCCTTTACCCTTCTCGAAGCCTTGGTCGCTCTCTTAGTGCTTAGTGGGGGAGTGTT
GGTCTTTCAAGGCTTGACCAAGCTTCTGCATGCTGAATTGGACTACCAGGCGCATCAAAAGCAGGAAGAATGGATACTTT
TTCAGCAACAATTGCAGGTGGAATTGGACCGGAGTCATTTTGAAAAAGTAGCGGACAATCATATCTACTTGGTTCAGGAT
CAAAAACCAATTGCTATTGGCCAATCTAAAGGAGATGATATCCGAAAAACAGATGATAAGGGCAGGGGCTATCAGCCTAT
GATTTCAGGTGTTCGCTCTAGCCAAATTTGGCAGGAGGGAGATTTGCTTCATCTGCGGTTGGATTTTGAAGAAGGTCTAG
AAAGGGAGTATGTCTACCATGTGGTACCAGCGATACAAAATGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (43-134)

3D structure

• AlphaFold DB: I1ZJT7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus mitis NCTC 12261	55.396	92.667	0.513
comGF/cglF	Streptococcus mitis SK321	54.676	92.667	0.507
comGF/cglF	Streptococcus pneumoniae Rx1	55.882	90.667	0.507
comGF/cglF	Streptococcus pneumoniae D39	55.882	90.667	0.507
comGF/cglF	Streptococcus pneumoniae R6	55.882	90.667	0.507
comGF/cglF	Streptococcus pneumoniae TIGR4	55.882	90.667	0.507
comYF	Streptococcus mutans UA159	42.647	90.667	0.387
comYF	Streptococcus mutans UA140	42.647	90.667	0.387
comGF	Lactococcus lactis subsp. cremoris KW2	39.007	94	0.367