Detailed information NTG0413780

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GTQ55_RS12175
• Genome accession: NZ_CP047491
• Coordinates: 2849340..2850524 (-)
• Length: 394 a.a.
• NCBI ID: WP_161858985.1
• Uniprot ID: A0A6P1TA59
• Organism: Microbulbifer hydrolyticus strain IRE-31
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2844340..2855524

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GTQ55_RS12150 (GTQ55_12150)	-	2845908..2846588 (+)	681	WP_161858980.1	zinc metallopeptidase	-
GTQ55_RS12155 (GTQ55_12155)	-	2846659..2847099 (+)	441	WP_161858981.1	GFA family protein	-
GTQ55_RS12160 (GTQ55_12160)	-	2847137..2847541 (+)	405	WP_161858982.1	GFA family protein	-
GTQ55_RS12165 (GTQ55_12165)	-	2847591..2848646 (+)	1056	WP_161858983.1	oxidoreductase	-
GTQ55_RS12170 (GTQ55_12170)	-	2848780..2849346 (+)	567	WP_161858984.1	SOUL family heme-binding protein	-
GTQ55_RS12175 (GTQ55_12175)	pilU	2849340..2850524 (-)	1185	WP_161858985.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
GTQ55_RS12180 (GTQ55_12180)	-	2851136..2852062 (+)	927	WP_161858986.1	alpha/beta hydrolase family esterase	-
GTQ55_RS12185 (GTQ55_12185)	-	2852219..2852848 (-)	630	WP_161858987.1	TetR/AcrR family transcriptional regulator	-
GTQ55_RS12190 (GTQ55_12190)	-	2852965..2854560 (-)	1596	WP_202620615.1	long-chain-fatty-acid--CoA ligase	-

Sequence

Protein

• Length: 394 a.a.
• Molecular weight: 44044.50 Da
• Isoelectric Point: 6.2015

>NTDB_id=413780 GTQ55_RS12175 WP_161858985.1 2849340..2850524(-) (pilU) [Microbulbifer hydrolyticus strain IRE-31]
MDFTGYLRVLAKNDGSDLYLSTGAPPCAKFQGVLKPLSKEPLGAGQIEKIAMEIMDEEQREEFKRELEMNLAMTIPNVGR
FRINIFWQRNQVSIVARNIVTEIPKFDDLKLPPILKDVVMAKRGLVLFVGGTGSGKSTSLAALIDHRNTNSGGHIITIED
PVEYVHKHKKSVINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLAISTLHANNANQALDRI
VNFFPEERRPQLLMSLSQNIRAFVSQRLVPTVDGKRCAAVEILLGTQTINELILKGEFHNIKEMMEKSENLGMQTFDGAL
FKLYVEGRISHEEAIKNADSANNLRLRIKMYEEGKKPKVAAAEAEDESEKTKAGRGLGLSLEALEDEEEESLVY

Nucleotide

• Length: 1185 bp

>NTDB_id=413780 GTQ55_RS12175 WP_161858985.1 2849340..2850524(-) (pilU) [Microbulbifer hydrolyticus strain IRE-31]
ATGGATTTTACCGGCTACCTCCGTGTGCTCGCCAAAAATGATGGCTCTGACCTCTACCTCAGTACCGGTGCGCCGCCCTG
CGCCAAATTCCAGGGGGTGCTGAAGCCCCTCAGCAAAGAGCCGCTCGGTGCCGGCCAGATCGAGAAGATCGCCATGGAGA
TCATGGACGAGGAGCAGCGGGAGGAGTTCAAGCGCGAGCTGGAAATGAACCTGGCGATGACCATCCCCAACGTAGGGCGT
TTCCGGATCAACATCTTCTGGCAGCGCAACCAGGTCTCGATCGTTGCACGAAATATCGTCACCGAGATCCCCAAATTTGA
CGACCTGAAGCTGCCGCCCATCCTCAAGGATGTGGTGATGGCCAAGCGCGGCCTGGTGCTGTTTGTCGGCGGTACGGGCT
CCGGCAAGTCCACCTCGCTGGCGGCACTGATTGACCATCGCAACACCAACAGCGGTGGTCACATTATCACCATCGAGGAC
CCGGTGGAGTACGTCCACAAACACAAGAAGAGCGTGATCAACCAGCGGGAAGTGGGGGTGGACACCCGCAGCTTCCATGC
CGCACTTAAGAACACCCTGCGTCAGGCGCCGGACGTAATCCTGATCGGCGAGATCCGCGACCGTGAGACCATGGAGCACG
CCATCGCCTTCGCCGAAACCGGCCATCTGGCGATCTCCACCCTGCACGCCAACAATGCCAACCAGGCTCTCGACCGGATT
GTGAACTTCTTTCCGGAGGAGCGTCGGCCGCAGTTGCTGATGTCGCTGTCACAGAACATCCGCGCGTTCGTGTCGCAGCG
CCTGGTGCCCACCGTGGATGGCAAGCGCTGTGCCGCGGTGGAAATCCTGCTGGGTACACAGACCATCAACGAGCTGATCC
TCAAGGGCGAGTTCCACAACATCAAGGAAATGATGGAAAAGTCGGAAAACCTCGGCATGCAGACGTTTGACGGCGCCCTG
TTCAAACTCTACGTGGAAGGCCGCATCAGCCACGAAGAAGCGATCAAGAATGCGGATTCCGCGAATAACCTGCGCCTGCG
TATCAAAATGTACGAAGAGGGCAAGAAGCCGAAGGTTGCCGCAGCGGAGGCAGAAGATGAAAGCGAAAAGACCAAAGCCG
GCAGGGGGCTCGGGCTGTCGCTGGAAGCACTGGAAGACGAAGAGGAAGAATCGCTGGTTTACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (66-270)

3D structure

• AlphaFold DB: A0A6P1TA59

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.367	89.848	0.497
pilU	Acinetobacter baylyi ADP1	53.76	91.117	0.49
pilU	Vibrio cholerae strain A1552	48.857	88.832	0.434
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	86.041	0.371
pilT	Legionella pneumophila strain Lp02	41.813	86.802	0.363
pilT	Legionella pneumophila strain ERS1305867	41.813	86.802	0.363