Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   H6S61_RS09685 Genome accession   NZ_CP060047
Coordinates   1943803..1945302 (+) Length   499 a.a.
NCBI ID   WP_011149205.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 2497-87     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1938803..1950302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H6S61_RS09665 (H6S61_09665) hslO 1939195..1940070 (-) 876 WP_017420504.1 Hsp33 family molecular chaperone HslO -
  H6S61_RS09670 (H6S61_09670) hslR 1940102..1940488 (-) 387 WP_011078946.1 ribosome-associated heat shock protein Hsp15 -
  H6S61_RS09675 (H6S61_09675) gspC 1940873..1941748 (+) 876 WP_157401904.1 type II secretion system protein GspC -
  H6S61_RS09680 (H6S61_09680) gspD 1941785..1943803 (+) 2019 WP_039541407.1 type II secretion system secretin GspD -
  H6S61_RS09685 (H6S61_09685) pilB/pilB1 1943803..1945302 (+) 1500 WP_011149205.1 type II secretion system ATPase GspE Machinery gene
  H6S61_RS09690 (H6S61_09690) gspF 1945302..1946519 (+) 1218 WP_011078942.1 type II secretion system inner membrane protein GspF -
  H6S61_RS09695 (H6S61_09695) gspG 1946573..1947016 (+) 444 WP_017420501.1 type II secretion system major pseudopilin GspG -
  H6S61_RS09700 (H6S61_09700) gspH 1946943..1947647 (+) 705 WP_238788312.1 type II secretion system minor pseudopilin GspH -
  H6S61_RS09705 (H6S61_09705) gspI 1947640..1948002 (+) 363 WP_185890329.1 type II secretion system minor pseudopilin GspI -
  H6S61_RS09710 (H6S61_09710) gspJ 1947989..1948690 (+) 702 WP_017789551.1 type II secretion system minor pseudopilin GspJ -
  H6S61_RS09715 (H6S61_09715) gspK 1948683..1949705 (+) 1023 WP_026130546.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55860.17 Da        Isoelectric Point: 6.0826

>NTDB_id=412737 H6S61_RS09685 WP_011149205.1 1943803..1945302(+) (pilB/pilB1) [Vibrio vulnificus strain 2497-87]
MVDILDTAPSYRRLPFSFANRFKMVLEIEHPERPPVLYYVEPLNAQALVEVRRVLKQTFVPQAIAAEAFEKKLTEAYQRD
SSEARQLMEDIGSDDDFFSLAEELPQDEDLLETEDDAPIIKLINAMLGEAIKEGASDIHIETFEKILSIRFRVDGVLRDV
LSPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTPV
NHDNFRHLISRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCQDCKEPY
EADKEQRKLFGAKKKDALTLYRAKGCEKCNHKGYRGRTGIHELLMVDDKVQELIHAEAGEQAMDKYIREHTPSIRSDGLD
KVLQGVTSLEEVMRVTKES

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=412737 H6S61_RS09685 WP_011149205.1 1943803..1945302(+) (pilB/pilB1) [Vibrio vulnificus strain 2497-87]
ATGGTAGATATACTGGATACGGCGCCATCGTATCGCCGTTTACCGTTTAGTTTTGCTAACCGCTTTAAAATGGTGCTGGA
AATCGAGCATCCAGAAAGGCCGCCGGTACTTTACTACGTTGAGCCTCTCAATGCGCAGGCGTTAGTTGAAGTGCGTCGTG
TGCTCAAACAGACGTTTGTCCCTCAAGCCATTGCCGCTGAAGCGTTTGAAAAAAAACTGACCGAAGCGTACCAACGCGAC
TCGTCAGAAGCGCGTCAGTTGATGGAGGACATTGGCTCGGATGATGACTTCTTCTCCTTGGCCGAAGAGCTGCCGCAAGA
TGAAGATTTGCTCGAAACGGAAGACGATGCACCGATCATCAAATTGATCAACGCCATGTTGGGCGAAGCGATCAAAGAAG
GTGCGTCGGATATTCACATCGAAACGTTTGAGAAAATTTTGTCGATTCGTTTCCGTGTCGATGGCGTTTTGCGCGATGTG
TTGTCTCCGAGCCGCAAACTGGCGCCGCTGTTGGTCTCTCGCGTTAAAGTCATGGCCAAACTCGACATCGCGGAAAAACG
AGTGCCACAAGATGGCCGTATTTCACTGCGTATCGGTGGTCGCGCGGTCGATGTGCGTGTATCGACCATGCCTTCATCAC
ACGGAGAACGTGTGGTAATGCGTTTGCTCGACAAAAACGCCACTCGCCTTGACCTGCATAGCTTGGGCATGACGCCGGTG
AACCACGACAATTTCCGTCATCTTATCTCGCGTCCTCACGGCATTATTTTGGTCACCGGTCCAACAGGTTCGGGTAAATC
CACCACCTTGTACGCTGGCTTGCAAGAGCTGAACAGCAACGAGCGCAACATCTTAACGGTGGAAGACCCGATCGAATTTG
ATATTGATGGCATTGGCCAAACCCAAGTGAACCCAAAAGTCGACATGACCTTTGCACGCGGTCTGCGAGCCATTTTGCGT
CAAGACCCAGACGTGGTGATGGTCGGGGAAATTCGTGACTTGGAAACCGCGCAAATTGCCGTACAAGCGTCATTGACCGG
TCACTTGGTGATGTCAACCTTGCACACCAACACCGCCGTAGGTGCGATTACCCGTCTGCGAGACATGGGCATTGAGCCTT
TTTTGATCTCTTCTTCACTGCTGGGCGTGCTGGCTCAGCGCTTGGTGCGGACCTTATGCCAAGATTGTAAAGAGCCGTAT
GAGGCTGATAAAGAGCAGCGCAAACTGTTTGGCGCGAAGAAGAAAGATGCGCTGACTTTATATCGTGCCAAGGGGTGTGA
AAAATGTAACCACAAAGGTTATCGTGGCCGTACGGGTATCCATGAGCTGTTGATGGTGGACGATAAAGTCCAAGAGCTGA
TCCATGCTGAGGCGGGAGAGCAGGCGATGGACAAATACATTCGTGAACATACCCCAAGCATTCGCAGCGATGGTTTAGAC
AAAGTGTTGCAAGGCGTGACCTCACTGGAAGAAGTGATGCGCGTGACCAAGGAATCTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

42.966

100

0.453
  pilF Thermus thermophilus HB27

44.915

94.589

0.425
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.51

98.998

0.421
  pilB Acinetobacter baumannii D1279779

42.292

96.192

0.407
  pilB Legionella pneumophila strain ERS1305867

46.934

84.97

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.348

79.559

0.369
  pilB Vibrio campbellii strain DS40M4

44.853

81.764

0.367
  pilB Vibrio cholerae strain A1552

45.96

79.359

0.365
  pilF Neisseria gonorrhoeae MS11

46.193

78.958

0.365
  pilB Acinetobacter baylyi ADP1

46.41

78.156

0.363

Multiple sequence alignment