Detailed information    

insolico Bioinformatically predicted

Overview


Name   comYF   Type   Machinery gene
Locus tag   GQY29_RS04115 Genome accession   NZ_CP047191
Coordinates   756558..756995 (+) Length   145 a.a.
NCBI ID   WP_084825661.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain EU01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 751558..761995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQY29_RS04085 (GQY29_04085) - 753232..753594 (+) 363 WP_023909982.1 DUF1033 family protein -
  GQY29_RS04090 (GQY29_04090) comYA 753675..754616 (+) 942 WP_014622005.1 competence type IV pilus ATPase ComGA Machinery gene
  GQY29_RS04095 (GQY29_04095) comYB 754498..755598 (+) 1101 WP_159340986.1 competence type IV pilus assembly protein ComGB Machinery gene
  GQY29_RS04100 (GQY29_04100) comYC 755595..755921 (+) 327 WP_023909980.1 competence type IV pilus major pilin ComGC Machinery gene
  GQY29_RS04105 (GQY29_04105) comYD 755881..756309 (+) 429 WP_171815055.1 competence type IV pilus minor pilin ComGD Machinery gene
  GQY29_RS04110 (GQY29_04110) comGE 756281..756574 (+) 294 WP_023909978.1 competence type IV pilus minor pilin ComGE -
  GQY29_RS04115 (GQY29_04115) comYF 756558..756995 (+) 438 WP_084825661.1 competence type IV pilus minor pilin ComGF Machinery gene
  GQY29_RS04120 (GQY29_04120) comGG 756973..757290 (+) 318 WP_023909975.1 competence type IV pilus minor pilin ComGG -
  GQY29_RS04125 (GQY29_04125) comYH 757335..758291 (+) 957 WP_023909974.1 class I SAM-dependent methyltransferase Machinery gene
  GQY29_RS04130 (GQY29_04130) - 758347..759540 (+) 1194 WP_084825660.1 acetate kinase -
  GQY29_RS04135 (GQY29_04135) - 759788..759985 (+) 198 WP_159340988.1 helix-turn-helix transcriptional regulator -
  GQY29_RS10680 (GQY29_04140) - 759997..760272 (+) 276 WP_100273190.1 CAAX protease -
  GQY29_RS10685 (GQY29_04145) - 760316..760654 (+) 339 WP_228024408.1 CPBP family intramembrane glutamic endopeptidase -
  GQY29_RS04150 (GQY29_04150) - 760728..761171 (+) 444 WP_041829335.1 hypothetical protein -
  GQY29_RS04155 (GQY29_04155) - 761268..761930 (+) 663 WP_084826165.1 type II CAAX endopeptidase family protein -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 16345.87 Da        Isoelectric Point: 9.3928

>NTDB_id=411388 GQY29_RS04115 WP_084825661.1 756558..756995(+) (comYF) [Streptococcus thermophilus strain EU01]
MYLSSKLRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKRVRQQENQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSASDFRKTNADGRGYQPMLFDVKSSKVSQKNQIVTIQVTLKNGLQRSFIYAFEKTG

Nucleotide


Download         Length: 438 bp        

>NTDB_id=411388 GQY29_RS04115 WP_084825661.1 756558..756995(+) (comYF) [Streptococcus thermophilus strain EU01]
ATGTATCTAAGCAGTAAGCTGCGTGCCTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTAGTCATTGCTGGCTCAGTTCA
AGTTTATCAAGGGTTAACCACTGTACTTGTGAGTAATGTTAAGCGGGTAAGACAACAAGAAAACCAAGACTGGCTTCTAT
TTGTTCAACAAATGGAGGCAGAATTAGAAGGATGTCAGTTGGTCAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTGGCTTTTGGTCTTTCAAGTGCCTCTGATTTTCGAAAAACAAATGCTGATGGTCGAGGCTATCAACCAAT
GCTTTTCGATGTCAAATCTAGTAAGGTAAGTCAAAAGAACCAGATAGTTACCATCCAAGTGACTCTTAAAAATGGACTAC
AAAGGAGTTTTATTTATGCTTTTGAAAAAACAGGTTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

58.156

97.241

0.566

  comYF Streptococcus mutans UA159

57.447

97.241

0.559

  comGF/cglF Streptococcus pneumoniae D39

50.714

96.552

0.49

  comGF/cglF Streptococcus pneumoniae Rx1

50.714

96.552

0.49

  comGF/cglF Streptococcus pneumoniae R6

50.714

96.552

0.49

  comGF/cglF Streptococcus pneumoniae TIGR4

50.714

96.552

0.49

  comGF/cglF Streptococcus mitis NCTC 12261

48.936

97.241

0.476

  comGF/cglF Streptococcus mitis SK321

48.227

97.241

0.469

  comGF Lactococcus lactis subsp. cremoris KW2

46.763

95.862

0.448


Multiple sequence alignment