Detailed information NTG0411158

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: GRT15_RS12240
• Genome accession: NZ_CP047157
• Coordinates: 2546679..2547293 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain FJAT-45028
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2541679..2552293

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GRT15_RS12210 (GRT15_12210)	rpsT	2541979..2542245 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
GRT15_RS12215 (GRT15_12215)	holA	2542262..2543303 (-)	1042	Protein_2380	DNA polymerase III subunit delta	-
GRT15_RS12220 (GRT15_12220)	-	2543343..2543497 (-)	155	Protein_2381	hypothetical protein	-
GRT15_RS12225 (GRT15_12225)	-	2543538..2543672 (+)	135	WP_003152870.1	YqzM family protein	-
GRT15_RS12230 (GRT15_12230)	comEC	2543691..2546042 (-)	2352	WP_043867296.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GRT15_RS12235 (GRT15_12235)	-	2546043..2546612 (-)	570	WP_003152868.1	ComE operon protein 2	-
GRT15_RS12240 (GRT15_12240)	comEA	2546679..2547293 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
GRT15_RS12245 (GRT15_12245)	comER	2547352..2548173 (+)	822	WP_044053476.1	late competence protein ComER	-
GRT15_RS12250 (GRT15_12250)	-	2548242..2548979 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
GRT15_RS12255 (GRT15_12255)	rsfS	2548976..2549332 (-)	357	WP_003152864.1	ribosome silencing factor	-
GRT15_RS12260 (GRT15_12260)	yqeK	2549329..2549910 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
GRT15_RS12265 (GRT15_12265)	-	2549900..2550469 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
GRT15_RS12270 (GRT15_12270)	yhbY	2550480..2550770 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
GRT15_RS12275 (GRT15_12275)	aroE	2550764..2551606 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=411158 GRT15_RS12240 WP_043867297.1 2546679..2547293(-) (comEA) [Bacillus velezensis strain FJAT-45028]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=411158 GRT15_RS12240 WP_043867297.1 2546679..2547293(-) (comEA) [Bacillus velezensis strain FJAT-45028]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363