Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GQ674_RS14795 Genome accession   NZ_CP047135
Coordinates   3272374..3274101 (-) Length   575 a.a.
NCBI ID   WP_159497680.1    Uniprot ID   A0A6P1EM00
Organism   Stenotrophomonas sp. 364     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3237210..3309666 3272374..3274101 within 0


Gene organization within MGE regions


Location: 3237210..3309666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQ674_RS14660 (GQ674_14660) - 3237754..3238248 (+) 495 WP_159497658.1 sigma-70 family RNA polymerase sigma factor -
  GQ674_RS14665 (GQ674_14665) - 3238238..3239212 (+) 975 WP_159497659.1 FecR domain-containing protein -
  GQ674_RS14670 (GQ674_14670) - 3239271..3242039 (+) 2769 WP_159497660.1 TonB-dependent receptor -
  GQ674_RS14675 (GQ674_14675) - 3242079..3243206 (+) 1128 WP_159497661.1 cytochrome c peroxidase -
  GQ674_RS14680 (GQ674_14680) - 3243216..3244043 (-) 828 WP_159497662.1 DUF3014 domain-containing protein -
  GQ674_RS14685 (GQ674_14685) - 3244252..3244962 (+) 711 WP_159497663.1 glutathione S-transferase -
  GQ674_RS14690 (GQ674_14690) - 3244964..3246574 (-) 1611 WP_159497664.1 hypothetical protein -
  GQ674_RS14695 (GQ674_14695) - 3246940..3248661 (-) 1722 WP_159497665.1 thiamine pyrophosphate-dependent enzyme -
  GQ674_RS14700 (GQ674_14700) - 3248779..3249069 (+) 291 WP_159497666.1 hypothetical protein -
  GQ674_RS14705 (GQ674_14705) - 3249085..3249396 (+) 312 WP_159497667.1 hypothetical protein -
  GQ674_RS14710 (GQ674_14710) - 3249404..3250270 (-) 867 WP_236546092.1 SH3 domain-containing protein -
  GQ674_RS14715 (GQ674_14715) - 3250306..3250794 (-) 489 WP_159497668.1 DUF3574 domain-containing protein -
  GQ674_RS14720 (GQ674_14720) - 3251034..3253484 (+) 2451 WP_159497669.1 TonB-dependent receptor -
  GQ674_RS14725 (GQ674_14725) otsB 3253686..3254444 (+) 759 WP_159497670.1 trehalose-phosphatase -
  GQ674_RS14730 (GQ674_14730) - 3254542..3256323 (+) 1782 WP_159497671.1 glycoside hydrolase family 15 protein -
  GQ674_RS14735 (GQ674_14735) otsA 3256320..3257684 (+) 1365 WP_159497672.1 alpha,alpha-trehalose-phosphate synthase (UDP-forming) -
  GQ674_RS14740 (GQ674_14740) - 3258911..3260248 (+) 1338 WP_159497673.1 OmpA family protein -
  GQ674_RS14745 (GQ674_14745) - 3260256..3260687 (-) 432 WP_159497674.1 thiol-disulfide oxidoreductase DCC family protein -
  GQ674_RS14750 (GQ674_14750) pgeF 3260692..3261471 (-) 780 WP_159497675.1 peptidoglycan editing factor PgeF -
  GQ674_RS14755 (GQ674_14755) rluD 3261471..3262466 (-) 996 WP_128097083.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  GQ674_RS14760 (GQ674_14760) - 3262583..3263467 (+) 885 WP_128097082.1 outer membrane protein assembly factor BamD -
  GQ674_RS14765 (GQ674_14765) - 3263654..3264895 (+) 1242 WP_236546093.1 lipase family protein -
  GQ674_RS14770 (GQ674_14770) - 3264933..3266567 (-) 1635 WP_159497676.1 NAD+ synthase -
  GQ674_RS14775 (GQ674_14775) sucD 3266793..3267668 (-) 876 WP_128097080.1 succinate--CoA ligase subunit alpha -
  GQ674_RS14780 (GQ674_14780) sucC 3267690..3268859 (-) 1170 WP_159497677.1 ADP-forming succinate--CoA ligase subunit beta -
  GQ674_RS14785 (GQ674_14785) - 3269211..3270824 (+) 1614 WP_159497678.1 ATP-binding protein -
  GQ674_RS14790 (GQ674_14790) pilR 3270913..3272298 (+) 1386 WP_159497679.1 sigma-54 dependent transcriptional regulator Regulator
  GQ674_RS14795 (GQ674_14795) pilB 3272374..3274101 (-) 1728 WP_159497680.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GQ674_RS14800 (GQ674_14800) - 3274162..3275088 (-) 927 WP_159497681.1 serine hydrolase domain-containing protein -
  GQ674_RS14805 (GQ674_14805) - 3275075..3276286 (-) 1212 WP_159497682.1 isoprenylcysteine carboxylmethyltransferase family protein -
  GQ674_RS14810 (GQ674_14810) comP 3276361..3276810 (-) 450 WP_159497683.1 pilin Machinery gene
  GQ674_RS14815 (GQ674_14815) pilC 3277168..3278427 (+) 1260 WP_159497684.1 type II secretion system F family protein Machinery gene
  GQ674_RS14820 (GQ674_14820) - 3278435..3279298 (+) 864 WP_159497685.1 A24 family peptidase -
  GQ674_RS14825 (GQ674_14825) coaE 3279309..3279926 (+) 618 WP_159497686.1 dephospho-CoA kinase -
  GQ674_RS14830 (GQ674_14830) - 3280023..3281390 (-) 1368 WP_159497687.1 HAMP domain-containing sensor histidine kinase -
  GQ674_RS14835 (GQ674_14835) - 3281353..3282030 (-) 678 WP_017356179.1 response regulator transcription factor -
  GQ674_RS14840 (GQ674_14840) - 3282039..3282503 (-) 465 WP_328803786.1 hypothetical protein -
  GQ674_RS14845 (GQ674_14845) rimK 3282757..3283650 (-) 894 WP_038686616.1 30S ribosomal protein S6--L-glutamate ligase -
  GQ674_RS14850 (GQ674_14850) - 3283754..3284914 (-) 1161 WP_159497689.1 hypothetical protein -
  GQ674_RS14855 (GQ674_14855) - 3285177..3285914 (+) 738 WP_159497690.1 hypothetical protein -
  GQ674_RS14860 (GQ674_14860) - 3286752..3288281 (-) 1530 WP_159497691.1 MobA/MobL family protein -
  GQ674_RS14865 (GQ674_14865) - 3288401..3290182 (+) 1782 WP_159497692.1 patatin-like phospholipase family protein -
  GQ674_RS14870 (GQ674_14870) tcmP 3290480..3291346 (+) 867 WP_159497693.1 three-Cys-motif partner protein TcmP -
  GQ674_RS14875 (GQ674_14875) - 3291356..3292096 (+) 741 WP_159497694.1 phage Gp37/Gp68 family protein -
  GQ674_RS14880 (GQ674_14880) - 3292294..3292896 (-) 603 WP_159497695.1 hypothetical protein -
  GQ674_RS14885 (GQ674_14885) - 3293054..3294391 (-) 1338 WP_159497696.1 hypothetical protein -
  GQ674_RS14890 (GQ674_14890) - 3294600..3294827 (-) 228 WP_159497697.1 hypothetical protein -
  GQ674_RS14895 (GQ674_14895) - 3294877..3295950 (-) 1074 WP_159497698.1 Shedu anti-phage system protein SduA domain-containing protein -
  GQ674_RS14900 (GQ674_14900) - 3295988..3296722 (-) 735 WP_236546094.1 hypothetical protein -
  GQ674_RS14905 (GQ674_14905) - 3296783..3296929 (-) 147 WP_201290298.1 hypothetical protein -
  GQ674_RS14910 (GQ674_14910) - 3297284..3298675 (+) 1392 WP_159497700.1 site-specific integrase -
  GQ674_RS14915 (GQ674_14915) - 3298785..3299570 (+) 786 WP_159497701.1 hypothetical protein -
  GQ674_RS14920 (GQ674_14920) - 3300939..3301064 (+) 126 Protein_2925 IS3 family transposase -
  GQ674_RS14925 (GQ674_14925) - 3301073..3301672 (-) 600 WP_159497702.1 CGNR zinc finger domain-containing protein -
  GQ674_RS14930 (GQ674_14930) - 3301750..3302727 (+) 978 WP_236546095.1 alpha/beta hydrolase -
  GQ674_RS14935 (GQ674_14935) - 3302741..3303100 (+) 360 Protein_2928 IS3 family transposase -
  GQ674_RS14940 (GQ674_14940) - 3303284..3303664 (-) 381 Protein_2929 H-NS family nucleoid-associated regulatory protein -
  GQ674_RS14945 (GQ674_14945) - 3304461..3305447 (+) 987 WP_159497704.1 hypothetical protein -
  GQ674_RS14950 (GQ674_14950) - 3305444..3305857 (-) 414 WP_159497705.1 nucleoid-associated protein -
  GQ674_RS14955 (GQ674_14955) - 3305920..3306255 (-) 336 WP_159497706.1 helix-turn-helix transcriptional regulator -
  GQ674_RS14960 (GQ674_14960) - 3306365..3307138 (+) 774 WP_159497707.1 hypothetical protein -
  GQ674_RS14965 (GQ674_14965) - 3307178..3307483 (+) 306 WP_159497708.1 hypothetical protein -
  GQ674_RS21645 - 3307462..3307845 (+) 384 WP_236546312.1 hypothetical protein -
  GQ674_RS14970 (GQ674_14970) - 3308523..3309083 (+) 561 WP_159497709.1 hypothetical protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62199.34 Da        Isoelectric Point: 6.2658

>NTDB_id=410925 GQ674_RS14795 WP_159497680.1 3272374..3274101(-) (pilB) [Stenotrophomonas sp. 364]
MSAVSTANLVGITGIARRLVQDGVLEEAAARDAMAKAGAAKQPLPQWFAEKKLVTAAQLAAANALEFGMPLLDVSVFDGS
QSAIALVSEELLRKHRVLPLFKRGGKLFVGTSDPTHSLDEIKFHTNLVVEPILVDEEQIKRTLEQWQSKHDTLGSTLGGD
DDGMGDLDVSTGDEEGGSTDSGVDAKGDDTPVVKFVNKVLIDAIRKGASDIHFEPYEDDYRVRFRIDGLLKNVAKAPVKL
NQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPAQQQLFL
DAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPFNITSSVTLVIAQRLARRLCSNCKRPIELPAHA
LLAEGFTQAQIDAGIRLFEPVGCEECTEGYKGRTGIYQVMPMSEEISVIVLAGGNAIQIAEAAQRAGVNDLRQSALVKAA
AGTTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=410925 GQ674_RS14795 WP_159497680.1 3272374..3274101(-) (pilB) [Stenotrophomonas sp. 364]
ATGTCCGCAGTCAGCACAGCCAACCTCGTCGGAATCACTGGTATTGCCCGTCGCCTCGTGCAGGACGGGGTGCTGGAAGA
GGCCGCTGCCCGGGACGCCATGGCGAAGGCCGGCGCTGCCAAGCAGCCTTTGCCGCAATGGTTCGCCGAGAAGAAGCTGG
TCACGGCCGCCCAGCTCGCCGCCGCGAACGCACTTGAATTCGGCATGCCCCTTCTGGATGTCTCCGTTTTCGACGGCAGC
CAAAGCGCCATCGCCCTCGTCAGCGAAGAACTCCTCCGCAAGCACCGCGTCCTGCCGCTGTTCAAGCGCGGGGGCAAGCT
GTTTGTGGGTACCAGTGACCCCACCCACTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTGGAGCCGATCCTGG
TCGACGAAGAGCAGATCAAGCGGACGCTAGAGCAGTGGCAGTCCAAGCACGACACGCTCGGTTCGACGCTGGGCGGCGAC
GACGACGGCATGGGCGACCTGGACGTGTCGACCGGTGACGAGGAGGGCGGTTCGACCGACTCCGGCGTGGACGCCAAGGG
CGACGATACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGATCGATGCGATCCGCAAGGGCGCCTCGGATATCCATTTTG
AGCCGTACGAAGACGATTACCGGGTGCGCTTCCGCATCGATGGCCTGCTCAAGAACGTGGCCAAGGCCCCGGTCAAGCTC
AACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTCGATATCGCTGAGAAGCGCGTGCCGCAGGATGGGCGCAT
CAAGCTCAACCTGTCCAAGACCCGCCAGATCGACTTCCGCGTGAGCACGCTGCCCACCCTGTTCGGCGAGAAGATCGTGC
TGCGTATCCTGGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGCCCAGCAGCAGTTGTTCCTG
GACGCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTATACCGC
GCTGGGCATCCTCAACGATGAAACGCGCAACATCTCCACCGCCGAAGATCCGGTGGAAATCCGCCTGCCCGGCGTGAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCGATCAAGGCCGCGCAGACAGGCCACATGGTGCTGTCCAC
GCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCGCCGTTCAACATCACCAGCTCGG
TCACGCTGGTGATCGCCCAGCGCCTGGCGCGGCGGCTGTGCAGCAACTGCAAGCGGCCGATCGAGCTGCCGGCGCATGCG
TTGCTGGCCGAAGGCTTCACCCAGGCGCAGATCGATGCGGGTATCCGTCTGTTCGAGCCGGTGGGGTGCGAGGAGTGCAC
CGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGAGTGAGGAAATCTCGGTGATCGTGCTGGCCGGTG
GCAATGCCATCCAGATCGCCGAGGCCGCGCAGCGCGCCGGGGTGAACGACCTGCGGCAGTCGGCGCTGGTCAAGGCGGCG
GCGGGCACCACCAGCCTGGCCGAGATCAACCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P1EM00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.228

100

0.565
  pilB Acinetobacter baumannii D1279779

56.055

100

0.563
  pilB Legionella pneumophila strain ERS1305867

51.585

98.783

0.51
  pilF Neisseria gonorrhoeae MS11

49.03

98.609

0.483
  pilB Vibrio cholerae strain A1552

48.682

98.957

0.482
  pilB Vibrio campbellii strain DS40M4

45.979

99.478

0.457
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

97.391

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

40.154

90.087

0.362

Multiple sequence alignment