Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GQS55_RS01915 Genome accession   NZ_CP047130
Coordinates   451228..452931 (-) Length   567 a.a.
NCBI ID   WP_159817432.1    Uniprot ID   -
Organism   Colwellia sp. 20A7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 446228..457931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQS55_RS01885 mutT 446336..446761 (+) 426 WP_159817420.1 8-oxo-dGTP diphosphatase MutT -
  GQS55_RS01890 yacG 446870..447106 (-) 237 WP_159817422.1 DNA gyrase inhibitor YacG -
  GQS55_RS01895 zapD 447200..447946 (-) 747 WP_159817424.1 cell division protein ZapD -
  GQS55_RS01900 coaE 448086..448697 (-) 612 WP_159817426.1 dephospho-CoA kinase -
  GQS55_RS01905 pilD 448783..449667 (-) 885 WP_159817428.1 A24 family peptidase Machinery gene
  GQS55_RS01910 pilC 449938..451185 (-) 1248 WP_159817430.1 type II secretion system F family protein Machinery gene
  GQS55_RS01915 pilB 451228..452931 (-) 1704 WP_159817432.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GQS55_RS01920 - 453095..453781 (+) 687 WP_159817434.1 transposase -
  GQS55_RS01925 - 453960..454418 (-) 459 WP_328698886.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GQS55_RS01930 rplM 455109..455537 (+) 429 WP_159817436.1 50S ribosomal protein L13 -
  GQS55_RS01935 rpsI 455541..455933 (+) 393 WP_114326795.1 30S ribosomal protein S9 -
  GQS55_RS01940 petA 457193..457783 (+) 591 WP_159817438.1 ubiquinol-cytochrome c reductase iron-sulfur subunit -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62505.68 Da        Isoelectric Point: 5.3353

>NTDB_id=410832 GQS55_RS01915 WP_159817432.1 451228..452931(-) (pilB) [Colwellia sp. 20A7]
MKGNHQQSSVLSALSTEDLIPLTNVDNIISDFTGKNKPFIRYLVEDKKVDGKKIAAILSYKFGYPYIQLSNFDTTLVPEG
VRNEKLIAKHNALPLFLRGKVLFVAMSDPTNLDALEEIQFNTGYSTELILTDELGLQACIEKVLEDESGSLNITDIDSNE
LSGIDTQEEQTEDDKVNTGEDDAPIVVFINKILLDAIKKGASDLHFEPYEKSFRIRFRIDGILTEIARPPVSLSSRMSAR
LKVMAKLDIAERRVPQDGRIKLSLSKKKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIDMLGYEPAQKKIYMEALEQPQ
GMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGINQVQINNRAGLTFPSALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPAYNVASSVTTIIAQRLARRLCSQCKTHEVLSETQLAEQGFP
ADKISEIKLFKPVGCDHCTGGYKGRVGIYEVIKISPKISSIIMEGGNSLDIAEQCQKEGYNNLRQSGLEKAMNGMTSLKE
VNRVTSS

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=410832 GQS55_RS01915 WP_159817432.1 451228..452931(-) (pilB) [Colwellia sp. 20A7]
ATGAAAGGCAATCACCAACAATCCAGTGTGTTATCCGCTTTATCTACAGAGGATCTAATCCCGCTTACTAATGTCGATAA
TATTATTAGTGACTTTACGGGTAAAAATAAACCTTTTATTCGCTATTTAGTGGAAGATAAAAAGGTTGACGGGAAAAAAA
TTGCCGCTATTTTATCGTATAAATTTGGCTACCCTTATATACAGCTGAGTAATTTCGATACAACCCTAGTGCCTGAAGGT
GTTCGTAATGAAAAGCTCATTGCTAAGCATAATGCCCTACCGTTATTTTTACGTGGCAAAGTGCTTTTTGTTGCCATGTC
TGACCCGACCAACCTTGATGCCTTAGAAGAAATACAATTTAATACTGGCTATAGCACTGAACTGATATTAACTGATGAAC
TTGGCTTACAAGCTTGTATTGAAAAAGTACTAGAAGATGAAAGTGGCTCTCTTAATATTACTGATATTGATTCTAATGAA
CTATCAGGTATAGATACTCAAGAAGAGCAAACTGAAGATGACAAAGTTAATACCGGTGAAGATGATGCACCTATTGTTGT
CTTTATTAATAAAATTTTATTAGATGCCATCAAAAAAGGGGCGTCTGATTTACACTTTGAACCTTATGAAAAATCTTTTC
GTATTCGTTTTCGTATTGATGGCATTTTAACTGAAATCGCCCGTCCGCCAGTAAGCTTATCGTCACGTATGTCAGCGCGT
TTAAAAGTGATGGCTAAACTTGATATCGCTGAGCGTAGAGTGCCACAAGATGGCAGAATAAAATTATCCCTTTCTAAGAA
AAAATCCATCGATTTCCGAGTAAGTACCTTGCCCACAATGTGGGGTGAAAAAATAGTAATGCGTATTCTCGACTCTTCAA
GTGCGATGCTCGGTATTGACATGTTAGGCTATGAGCCTGCACAGAAAAAAATCTATATGGAAGCATTAGAGCAGCCTCAA
GGCATGATTTTAGTTACAGGCCCAACGGGCTCCGGTAAAACAGTTTCTTTATATACCGGCTTGAATATTCTTAATACTGC
TGAACGTAATATTTCTACTGCAGAAGATCCCGTAGAAATTAATTTAGAAGGTATTAATCAAGTACAAATTAACAACCGAG
CGGGACTAACTTTTCCAAGCGCATTACGTTCTTTTTTACGTCAAGATCCTGATATTGTCATGGTTGGTGAAATTCGAGAC
TTAGAAACAGCTGAAATAGCAATTAAAGCCGCCCAAACAGGTCATTTAGTATTATCTACCTTGCATACTAACTCTGCTGC
TGAAACATTAACGCGTTTACTTAATATGGGCGTGCCTGCTTATAACGTTGCCAGTTCAGTAACAACTATTATTGCTCAGC
GGCTAGCAAGGCGTTTATGCTCACAATGCAAAACGCACGAAGTGCTTTCAGAAACACAACTTGCTGAACAAGGCTTTCCT
GCCGATAAGATAAGTGAAATTAAACTATTTAAACCTGTTGGTTGTGACCACTGTACGGGTGGTTATAAAGGCCGAGTGGG
TATTTATGAAGTTATTAAAATTAGCCCTAAAATATCCTCAATTATTATGGAAGGTGGTAATTCATTAGATATTGCTGAGC
AGTGTCAAAAAGAAGGTTATAATAATTTAAGGCAGTCAGGCTTAGAAAAAGCAATGAATGGTATGACTAGCCTTAAAGAA
GTTAACCGCGTAACCAGTTCTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.134

93.651

0.554
  pilB Acinetobacter baumannii D1279779

58.79

93.298

0.548
  pilB Legionella pneumophila strain ERS1305867

57.25

93.651

0.536
  pilB Vibrio cholerae strain A1552

51.237

99.824

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

49.201

99.295

0.489
  pilB Vibrio campbellii strain DS40M4

53.021

90.476

0.48
  pilF Neisseria gonorrhoeae MS11

49.527

93.298

0.462
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.813

94.356

0.376

Multiple sequence alignment