Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GQS55_RS01905 Genome accession   NZ_CP047130
Coordinates   448783..449667 (-) Length   294 a.a.
NCBI ID   WP_159817428.1    Uniprot ID   -
Organism   Colwellia sp. 20A7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 443783..454667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQS55_RS01885 mutT 446336..446761 (+) 426 WP_159817420.1 8-oxo-dGTP diphosphatase MutT -
  GQS55_RS01890 yacG 446870..447106 (-) 237 WP_159817422.1 DNA gyrase inhibitor YacG -
  GQS55_RS01895 zapD 447200..447946 (-) 747 WP_159817424.1 cell division protein ZapD -
  GQS55_RS01900 coaE 448086..448697 (-) 612 WP_159817426.1 dephospho-CoA kinase -
  GQS55_RS01905 pilD 448783..449667 (-) 885 WP_159817428.1 A24 family peptidase Machinery gene
  GQS55_RS01910 pilC 449938..451185 (-) 1248 WP_159817430.1 type II secretion system F family protein Machinery gene
  GQS55_RS01915 pilB 451228..452931 (-) 1704 WP_159817432.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GQS55_RS01920 - 453095..453781 (+) 687 WP_159817434.1 transposase -
  GQS55_RS01925 - 453960..454418 (-) 459 WP_328698886.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 294 a.a.        Molecular weight: 33171.57 Da        Isoelectric Point: 7.2758

>NTDB_id=410830 GQS55_RS01905 WP_159817428.1 448783..449667(-) (pilD) [Colwellia sp. 20A7]
MFEQTLLFFQQSPFSFYIFVALFSLIIGSFLNVVVYRFPQMMHNTWYTDCREFLADELKDIPAKKIAPLTLSIPNSTCLK
CDHKIRFYENIPVISWLFLRGKCSQCKNSIAARYPLVEAATALLSVVTAIQFGVSIETLLLLILTWGLICLTLIDFDHML
LPDQIVMPLLWLGLLVNLQGTFVPLTDAVIGAAVGYMSLFSIFWLFKVLTGREGMGHGDFKLFALFGAWIGWQLLPLLIL
MASVVGAVVGISLMLFKNHQREQAIPFGPYLAVSGWITLLWGNGIWAWYIAQIS

Nucleotide


Download         Length: 885 bp        

>NTDB_id=410830 GQS55_RS01905 WP_159817428.1 448783..449667(-) (pilD) [Colwellia sp. 20A7]
ATGTTTGAGCAAACGTTATTATTTTTTCAACAATCCCCCTTTTCTTTCTACATTTTTGTTGCACTATTTTCATTGATCAT
TGGCAGTTTTTTAAATGTTGTGGTCTACCGTTTCCCCCAAATGATGCACAACACGTGGTATACCGATTGTCGTGAGTTTT
TAGCTGATGAATTGAAAGATATTCCAGCCAAAAAAATTGCGCCACTTACCTTGTCTATTCCTAATTCAACCTGTCTAAAG
TGTGACCATAAAATACGGTTTTATGAAAATATCCCGGTAATTAGTTGGTTGTTCCTACGTGGAAAATGTAGCCAATGTAA
AAATAGTATTGCTGCACGCTATCCTTTGGTGGAAGCAGCTACTGCCTTGCTAAGCGTTGTGACAGCTATACAGTTTGGTG
TCTCTATAGAAACCTTGCTACTGCTTATATTAACATGGGGATTGATTTGTTTAACCTTAATTGATTTTGATCACATGCTG
TTACCAGACCAAATAGTGATGCCACTGTTATGGCTTGGCTTGTTAGTAAACTTACAAGGAACTTTTGTACCACTAACCGA
TGCAGTTATTGGTGCGGCAGTCGGTTACATGAGTTTGTTTAGTATATTTTGGCTGTTTAAAGTATTAACCGGTAGAGAAG
GCATGGGTCATGGTGATTTCAAGCTATTTGCTTTGTTCGGTGCCTGGATTGGCTGGCAGTTACTACCCCTTCTTATTTTA
ATGGCGTCGGTAGTTGGTGCCGTTGTCGGTATTTCCTTAATGTTATTTAAAAATCATCAACGTGAACAAGCCATTCCTTT
TGGGCCTTACTTAGCTGTTTCGGGTTGGATAACATTATTGTGGGGCAACGGGATCTGGGCTTGGTATATTGCTCAAATAA
GTTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-132)

Type IV leader peptidase family (Peptidase_A24)

(142-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

50.699

97.279

0.493
  pilD Vibrio cholerae strain A1552

50.719

94.558

0.48
  pilD Acinetobacter nosocomialis M2

46.713

98.299

0.459
  pilD Acinetobacter baumannii D1279779

46.367

98.299

0.456
  pilD Neisseria gonorrhoeae MS11

45.818

93.537

0.429

Multiple sequence alignment