Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GO485_RS21265 Genome accession   NZ_CP046904
Coordinates   4938581..4940266 (-) Length   561 a.a.
NCBI ID   WP_229418555.1    Uniprot ID   A0A562Q3J6
Organism   Pseudoduganella flava strain DSM 26639     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4933581..4945266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO485_RS21250 (GO485_21205) - 4934918..4936231 (+) 1314 WP_145872721.1 peptide MFS transporter -
  GO485_RS21255 (GO485_21210) astA 4936233..4937264 (+) 1032 WP_145872722.1 arginine N-succinyltransferase -
  GO485_RS21260 (GO485_21215) pilC 4937343..4938572 (-) 1230 WP_145872723.1 type II secretion system F family protein Machinery gene
  GO485_RS21265 (GO485_21220) pilB 4938581..4940266 (-) 1686 WP_229418555.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GO485_RS21270 (GO485_21225) - 4940363..4941649 (-) 1287 WP_145872725.1 HlyC/CorC family transporter -
  GO485_RS21275 (GO485_21230) - 4941857..4942711 (+) 855 WP_145872726.1 helix-turn-helix domain-containing protein -
  GO485_RS21280 (GO485_21235) - 4942781..4943272 (+) 492 WP_145872727.1 NADH-quinone oxidoreductase subunit B family protein -
  GO485_RS21285 (GO485_21240) - 4943325..4944539 (+) 1215 WP_145872728.1 glutamate carboxypeptidase -
  GO485_RS21290 (GO485_21245) uraH 4944524..4944877 (-) 354 WP_145873262.1 hydroxyisourate hydrolase -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 60906.65 Da        Isoelectric Point: 6.1990

>NTDB_id=409264 GO485_RS21265 WP_229418555.1 4938581..4940266(-) (pilB) [Pseudoduganella flava strain DSM 26639]
MPGLARALLQAGRLTAPQADALQRKSHVEKAPFIDVLLASGAIASRELAVFCAETFAYPMLDLQAFAVAALPQKIIDPKL
MQTQRVVALARRGNKMSVAISDPTNTQALDQIKFQTETLVEPVIVPHDVLVRLLQELAKSSEQLIGELAGEEGDIQFEQE
PDAALQEAPATDVEDAPIVRFLNKMLMDAVNMGASDLHFEPFEKFYRIRFRVDGVLIEHAQPPIAIKDKLVSRIKVLAKL
DISEKRVPQDGRMRLIVSPTKTIDLRVSTLPTLFGEKTVMRILDATQAQMGIDSLGYEPEQRALLLDAIQRPYGMVLVTG
PTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLPGVNQVNVNDKAGLTFPVALKSFLRQDPDIIMVGEIRDLETADIA
IKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCSCKKPADIPADRLVSAGFRPEELDGSW
KPYAPAGCERCNGSGYKGRVGIYQIMPITPAIEALILAHGNSMQIAAQAQAEGVKSLRQSGLVKVKAGLTSLEEVLGCTN
E

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=409264 GO485_RS21265 WP_229418555.1 4938581..4940266(-) (pilB) [Pseudoduganella flava strain DSM 26639]
ATGCCGGGACTCGCGCGGGCATTGCTGCAGGCCGGGCGTTTGACCGCCCCGCAGGCGGATGCGCTGCAACGCAAGTCGCA
TGTGGAAAAGGCGCCGTTCATCGATGTGCTGCTGGCCAGCGGCGCGATCGCCTCGCGCGAGCTGGCCGTGTTCTGCGCGG
AGACGTTCGCGTACCCGATGCTCGACCTGCAGGCGTTCGCCGTGGCTGCGCTGCCACAGAAGATCATCGATCCGAAGCTG
ATGCAGACGCAGCGCGTGGTGGCGCTGGCCAGGCGCGGCAACAAGATGTCGGTGGCGATCTCGGATCCGACGAACACGCA
GGCGCTCGACCAGATCAAGTTCCAGACGGAGACGCTGGTCGAACCGGTGATCGTGCCGCACGACGTGCTGGTGCGGCTGC
TGCAGGAACTGGCCAAGAGCAGCGAGCAGCTGATCGGCGAACTGGCCGGCGAGGAAGGCGATATCCAGTTCGAGCAGGAA
CCCGATGCGGCGCTGCAGGAAGCGCCGGCGACGGACGTCGAGGATGCGCCGATCGTGCGCTTCCTGAACAAGATGCTGAT
GGACGCGGTGAACATGGGCGCGTCCGACCTGCACTTCGAACCGTTCGAGAAGTTCTACCGGATCCGCTTTCGCGTCGACG
GCGTGCTGATCGAACATGCGCAGCCGCCGATCGCGATCAAGGACAAGCTGGTGTCGCGCATCAAGGTGCTGGCCAAGCTG
GACATCTCGGAAAAGCGCGTGCCGCAGGATGGCCGGATGCGGCTGATCGTGTCGCCGACGAAGACGATCGACCTGCGCGT
CTCCACATTGCCGACGCTGTTCGGCGAGAAGACGGTGATGCGTATCCTCGATGCGACGCAGGCGCAGATGGGCATCGACT
CGCTGGGCTACGAGCCGGAACAACGCGCGCTGCTGCTCGACGCGATCCAACGGCCCTACGGCATGGTGCTCGTGACGGGG
CCGACGGGTTCCGGCAAGACGGTGTCGCTGTACACGTGCCTGAACATCCTGAACAAGCCGGGCATCAACATCTCGACGGC
CGAAGATCCCGCCGAGATCAACCTGCCCGGCGTGAACCAGGTGAACGTGAACGACAAGGCGGGGCTGACGTTCCCGGTCG
CGCTGAAGTCGTTCCTGCGCCAGGACCCGGACATCATCATGGTCGGCGAGATCCGCGACCTGGAAACGGCGGACATCGCG
ATCAAGGCGGCGCAGACGGGCCATATGGTGTTCTCGACCTTGCACACGAACGACGCGCCGTCGACGTTGACGCGCCTGAT
GAACATGGGCGTGGCGCCGTTCAACATCGCGTCGTCCGTCATCCTGATCACGGCGCAGCGCCTGGCGCGCCGGCTGTGCT
CCTGCAAGAAGCCGGCCGACATCCCGGCGGACCGCCTGGTCAGCGCCGGCTTCCGGCCGGAAGAACTGGACGGCAGCTGG
AAGCCCTACGCGCCGGCGGGCTGCGAGCGCTGCAACGGCTCGGGCTACAAGGGCCGCGTGGGCATCTACCAGATCATGCC
GATCACGCCGGCGATCGAGGCGCTGATCCTCGCGCACGGCAATTCGATGCAGATCGCCGCGCAGGCGCAAGCCGAGGGCG
TGAAGTCGCTGCGGCAGTCCGGCCTCGTGAAGGTGAAGGCGGGATTGACGAGTCTTGAAGAAGTGCTGGGCTGCACGAAC
GAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-142)

Type II/IV secretion system protein (T2SSE)

(184-451)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A562Q3J6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.27

100

0.544
  pilF Neisseria gonorrhoeae MS11

53.381

100

0.535
  pilB Acinetobacter baumannii D1279779

53.108

100

0.533
  pilB Legionella pneumophila strain ERS1305867

49.02

100

0.49
  pilB Vibrio cholerae strain A1552

46.512

99.643

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

45.745

100

0.46
  pilB Vibrio campbellii strain DS40M4

45.23

100

0.456
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.192

100

0.398
  pilF Thermus thermophilus HB27

37.899

95.009

0.36

Multiple sequence alignment