Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E3Z27_RS24015 Genome accession   NZ_CP046874
Coordinates   5523735..5525435 (+) Length   566 a.a.
NCBI ID   WP_047698302.1    Uniprot ID   -
Organism   Pseudomonas mediterranea strain S58     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5518735..5530435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3Z27_RS23995 (E3Z27_24005) - 5518907..5521840 (+) 2934 WP_055127680.1 DUF748 domain-containing protein -
  E3Z27_RS24000 (E3Z27_24010) - 5521842..5522138 (+) 297 WP_047698296.1 DUF2845 domain-containing protein -
  E3Z27_RS24005 (E3Z27_24015) - 5522438..5522791 (-) 354 WP_047698298.1 BON domain-containing protein -
  E3Z27_RS24010 (E3Z27_24020) - 5523089..5523511 (-) 423 WP_047698300.1 pilin -
  E3Z27_RS24015 (E3Z27_24025) pilB 5523735..5525435 (+) 1701 WP_047698302.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E3Z27_RS24020 (E3Z27_24030) pilC 5525438..5526655 (+) 1218 WP_047698304.1 type II secretion system F family protein Machinery gene
  E3Z27_RS24025 (E3Z27_24035) pilD 5526657..5527526 (+) 870 WP_047698305.1 prepilin peptidase Machinery gene
  E3Z27_RS24030 (E3Z27_24040) coaE 5527537..5528160 (+) 624 WP_047698307.1 dephospho-CoA kinase -
  E3Z27_RS24035 (E3Z27_24045) yacG 5528157..5528363 (+) 207 WP_047698308.1 DNA gyrase inhibitor YacG -
  E3Z27_RS24040 (E3Z27_24050) - 5528360..5528575 (-) 216 WP_047698310.1 hypothetical protein -
  E3Z27_RS24045 (E3Z27_24055) - 5528639..5529325 (-) 687 WP_047698311.1 energy-coupling factor ABC transporter permease -
  E3Z27_RS24050 (E3Z27_24060) - 5529545..5530171 (+) 627 WP_003185538.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62595.50 Da        Isoelectric Point: 5.2296

>NTDB_id=409075 E3Z27_RS24015 WP_047698302.1 5523735..5525435(+) (pilB) [Pseudomonas mediterranea strain S58]
MNDIALSGLTKQLVLAELITEQSAQQAQQQAQRNRMPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKESQPTGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSTTGLEGMGDVDLDG
LDIESDDDRKQDAIAGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRVRTDGMLREVARPPTQLATRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKELYLAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCSQCKKATEIPEETLLKEGFPK
ERIGSFTIYEPVGCEQCNSGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=409075 E3Z27_RS24015 WP_047698302.1 5523735..5525435(+) (pilB) [Pseudomonas mediterranea strain S58]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAACTCGTGCTGGCCGAGCTGATCACCGAGCAAAGCGCGCAGCAGGC
GCAGCAGCAAGCCCAACGCAACCGCATGCCCCTGGTCAGCTATCTGGTGCAGAACAAGCTGGTCCAGAGTCGCCAGGTTG
CGGAAATCGCCTCGGAGCATTTCGGCGTTGCCCTGCTGGACCTCAACAGCCTGGACAAGGAAAGCCAGCCCACCGGCCTG
GTCAGTGAAAAACTGGTCCGGCAACACCATGCCCTGCCGCTGTGGCGACGTGGCAACAAGCTGTTCGTGGGCATCTCCGA
TCCCACCAACCACCAGGCCATCAACGACATCCAGTTCAGTACCGGCCTGACCACCGAAGCCATCCTGGTGGAAGACGACA
AACTCAGCGACGCCATCGAGAAGTTTTTCGAATCCAGCACCACGGGCCTGGAAGGCATGGGCGATGTCGACCTCGATGGC
CTGGACATCGAGTCGGACGACGATCGCAAACAGGACGCCATCGCCGGTCAGGACGCCGATGACGCGCCGGTGGTGCGGTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCTTCCGACCTGCACTTCGAACCTTACGAAAAATCCTACCGGG
TGCGGGTGCGCACCGACGGCATGTTGCGGGAAGTGGCCAGGCCGCCGACCCAACTGGCCACCCGCATCGCGGCGCGCCTG
AAGGTCATGGCCAGCCTGGATATTTCCGAGCGGCGCAAGCCGCAGGACGGCCGGTTGAAAATGCGCCTGTCGAAAACCAA
GTCCATCGATTTCCGGGTCAATACCCTGCCTACGCTCTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCGTCCAGTG
CGCAAATGGGCATCGATGCCCTGGGCTACGAACCGGATCAAAAGGAGCTCTACCTCGCAGCCCTCAAGCAACCACAAGGG
CTGATCCTGGTGACCGGGCCGACCGGCTCGGGCAAGACTGTCTCGCTCTACACCGGCTTGAACATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTGAATCCGCGTCAGG
GACTGGACTTTGCCCAGGCCCTGCGCTCGTTCCTGCGTCAGGATCCGGACGTGATCATGGTGGGTGAGATCCGCGACCTG
GAAACGGCGGAAATCGCCATCAAGGCGGCCCAGACCGGGCACATGGTGCTTTCCACGCTGCACACCAACAGTGCCGCGGA
AACCTTGATCCGCTTGCAGAACATGGGCATTCCCGGCTTCAACATCGCCACGGCGGTCCACCTGATCATCGCCCAGCGCC
TGGCGCGAAAACTGTGCAGCCAATGCAAGAAAGCCACGGAGATTCCCGAGGAAACCCTGCTCAAGGAAGGCTTCCCCAAG
GAACGCATCGGCTCATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACAGTGGCTACAAGGGACGCGTGGGGGT
TTACGAAGTGGTTAAAAATACGCCCGAACTGCAACGGCTGATCATGGCCGAGGGCAATTCCCTGGAAATCGACCTGCAAA
TGCGCAAGGACGGTTTCAACGACCTGCGCACTTCGGGGCTGCTGAAAGTGATGCAAGGCGTGACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.333

99.647

0.581
  pilB Acinetobacter baumannii D1279779

57.672

100

0.578
  pilB Legionella pneumophila strain ERS1305867

55.026

100

0.551
  pilB Vibrio cholerae strain A1552

50.353

100

0.504
  pilB Vibrio campbellii strain DS40M4

48.319

99.823

0.482
  pilF Neisseria gonorrhoeae MS11

48.49

99.47

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.002

97.35

0.477

Multiple sequence alignment