Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   E3Z27_RS24020 Genome accession   NZ_CP046874
Coordinates   5525438..5526655 (+) Length   405 a.a.
NCBI ID   WP_047698304.1    Uniprot ID   A0AAX2D8R8
Organism   Pseudomonas mediterranea strain S58     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5520438..5531655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3Z27_RS24000 (E3Z27_24010) - 5521842..5522138 (+) 297 WP_047698296.1 DUF2845 domain-containing protein -
  E3Z27_RS24005 (E3Z27_24015) - 5522438..5522791 (-) 354 WP_047698298.1 BON domain-containing protein -
  E3Z27_RS24010 (E3Z27_24020) - 5523089..5523511 (-) 423 WP_047698300.1 pilin -
  E3Z27_RS24015 (E3Z27_24025) pilB 5523735..5525435 (+) 1701 WP_047698302.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E3Z27_RS24020 (E3Z27_24030) pilC 5525438..5526655 (+) 1218 WP_047698304.1 type II secretion system F family protein Machinery gene
  E3Z27_RS24025 (E3Z27_24035) pilD 5526657..5527526 (+) 870 WP_047698305.1 prepilin peptidase Machinery gene
  E3Z27_RS24030 (E3Z27_24040) coaE 5527537..5528160 (+) 624 WP_047698307.1 dephospho-CoA kinase -
  E3Z27_RS24035 (E3Z27_24045) yacG 5528157..5528363 (+) 207 WP_047698308.1 DNA gyrase inhibitor YacG -
  E3Z27_RS24040 (E3Z27_24050) - 5528360..5528575 (-) 216 WP_047698310.1 hypothetical protein -
  E3Z27_RS24045 (E3Z27_24055) - 5528639..5529325 (-) 687 WP_047698311.1 energy-coupling factor ABC transporter permease -
  E3Z27_RS24050 (E3Z27_24060) - 5529545..5530171 (+) 627 WP_003185538.1 DUF1780 domain-containing protein -
  E3Z27_RS24055 (E3Z27_24065) - 5530273..5530446 (+) 174 WP_003185543.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44044.70 Da        Isoelectric Point: 9.9440

>NTDB_id=409076 E3Z27_RS24020 WP_047698304.1 5525438..5526655(+) (pilC) [Pseudomonas mediterranea strain S58]
MAVKAVKTDTYTWEGKDRKGMKMSGELTGQSPALVKAQLRKQGINPEKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNANMRKLVDEVKQEVAAGNSFAASLRKCPQYFDELYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPAAVVVVAAVVTGILLVKVVPQFESVFAGFGAKLPAFTVMVIGLSEFMQDSWWLLLGGAVGAFFGVK
YALKRSQGFRDWRDKWLLKLPLVGALMYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIRQDVSTGM
QLNFSMRASGIFPNLAIQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVILGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=409076 E3Z27_RS24020 WP_047698304.1 5525438..5526655(+) (pilC) [Pseudomonas mediterranea strain S58]
ATGGCGGTCAAGGCAGTAAAAACCGACACCTACACCTGGGAAGGCAAAGACCGCAAAGGCATGAAGATGTCCGGTGAACT
GACCGGCCAGAGCCCGGCCCTGGTCAAGGCGCAATTGCGCAAACAAGGCATCAACCCGGAAAAGGTACGCAAGAAATCCA
CCTCGATATTCAGCAAGGGCAAACGCATCAAGCCACTGGATATCGCCCTCTTCACCCGCCAGATGGCGACGATGCTCAAA
GCCGGTGTCCCGCTGCTGCAAGCCTTCGACATCATTGGCGAGGGCTTCGACAATGCCAACATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAGGTCGCCGCAGGCAACAGTTTCGCCGCGTCGTTGCGCAAGTGCCCGCAATACTTCGATGAGCTGTACT
GCAACCTGGTGGACGCAGGTGAACAGGCCGGCGCCCTGGACACCTTGCTGGACCGGGTCGCGACCTACAAGGAAAAGAGC
GAGGCCCTCAAGGCCAAGATCAAGAAAGCCATGACCTATCCGGCGGCCGTTGTGGTCGTCGCGGCGGTGGTCACCGGCAT
TCTGCTGGTCAAGGTGGTGCCGCAATTCGAATCGGTGTTCGCCGGGTTCGGCGCGAAACTGCCTGCGTTCACCGTGATGG
TCATCGGCCTGTCTGAGTTCATGCAAGACTCATGGTGGCTGCTGCTGGGCGGGGCAGTGGGTGCGTTTTTTGGCGTGAAG
TATGCGCTCAAGCGCTCCCAGGGCTTTCGTGACTGGCGCGATAAATGGCTGCTCAAGCTACCGCTGGTGGGCGCGCTGAT
GTACAAATCCGCGGTAGCCCGCTACGCCCGGACACTGTCCACCACCTTCGCCGCCGGCGTGCCACTGGTGGAGGCGTTGG
ACTCGGTCTCCGGCGCCACCGGCAACGTGGTGTTCAAACGTGCGGTCCAGCGCATCCGGCAGGATGTCTCGACCGGCATG
CAGTTGAATTTTTCCATGCGCGCCTCAGGCATATTCCCCAACCTGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
GGCGTTGGACGACATGCTCGACAAGGTGGCAAGTTTCTATGAGGCCGAGGTCGACAACCTGGTGGACAATCTCACCAGCC
TGATGGAACCGTTCATCATGGTGATCCTGGGGGTGGTCGTCGGCGGTCTGGTGGTTGCCATGTACCTGCCCATCTTTCAA
CTCGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.827

100

0.738
  pilC Acinetobacter baylyi ADP1

60.25

98.765

0.595
  pilC Acinetobacter baumannii D1279779

58.824

100

0.593
  pilC Legionella pneumophila strain ERS1305867

55.164

98.025

0.541
  pilC Vibrio campbellii strain DS40M4

43.489

100

0.437
  pilG Neisseria gonorrhoeae MS11

43.035

99.259

0.427
  pilC Vibrio cholerae strain A1552

42.469

100

0.425
  pilG Neisseria meningitidis 44/76-A

42.469

100

0.425
  pilC Thermus thermophilus HB27

37.75

98.765

0.373

Multiple sequence alignment