Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPY16_RS03335 Genome accession   NZ_CP046832
Coordinates   678266..678703 (-) Length   145 a.a.
NCBI ID   WP_038941421.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 06-2410     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 673266..683703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY16_RS03315 coaE 673727..674335 (-) 609 WP_039536087.1 dephospho-CoA kinase -
  GPY16_RS03320 pilD 674337..675206 (-) 870 WP_038941424.1 A24 family peptidase Machinery gene
  GPY16_RS03325 pilC 675300..676526 (-) 1227 WP_061058909.1 type II secretion system F family protein Machinery gene
  GPY16_RS03330 pilB 676574..678262 (-) 1689 WP_158104985.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY16_RS03335 pilA 678266..678703 (-) 438 WP_038941421.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPY16_RS03340 nadC 678926..679813 (-) 888 WP_072600771.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY16_RS03345 ampD 679906..680478 (+) 573 WP_102982150.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY16_RS03350 pdhR 680867..681634 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14916.93 Da        Isoelectric Point: 7.8162

>NTDB_id=408592 GPY16_RS03335 WP_038941421.1 678266..678703(-) (pilA) [Vibrio vulnificus strain 06-2410]
MKKLNKTKKQQGFTLIELMIVVAIIGVLAAVAVPAYQDYVKKSEAASALATLKSLITPAELYYQENGTATAAGLSDLGST
TSANDLGTLASAITGSGSAAVPTLTFSFGSHSSMTTTDKLTFSRSNTEGWSCARSGTVPAVDGCQ

Nucleotide


Download         Length: 438 bp        

>NTDB_id=408592 GPY16_RS03335 WP_038941421.1 678266..678703(-) (pilA) [Vibrio vulnificus strain 06-2410]
ATGAAAAAATTAAACAAAACCAAGAAACAACAAGGTTTTACGTTGATTGAGTTAATGATCGTAGTGGCAATCATTGGTGT
GTTAGCCGCAGTTGCAGTTCCTGCATACCAAGACTATGTCAAAAAGTCAGAAGCTGCCTCAGCCCTCGCAACATTGAAAT
CTTTGATCACACCAGCAGAGTTATATTATCAAGAAAACGGAACTGCAACAGCTGCTGGTTTGTCCGATTTAGGTTCAACT
ACGTCAGCAAATGATTTGGGCACTTTGGCTTCAGCCATTACTGGTTCAGGTTCAGCAGCTGTTCCAACATTGACATTTTC
ATTTGGTTCACATAGTTCAATGACTACAACAGACAAATTAACATTTTCTAGAAGCAATACTGAAGGTTGGTCATGCGCTC
GCAGTGGTACGGTACCAGCAGTAGATGGTTGCCAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-139)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

61.972

97.931

0.607
  pilA Pseudomonas aeruginosa PAK

43.284

92.414

0.4
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.876

94.483

0.386
  pilA Vibrio cholerae strain A1552

40.876

94.483

0.386
  pilA Vibrio cholerae C6706

40.876

94.483

0.386

Multiple sequence alignment