Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPY15_RS11745 Genome accession   NZ_CP046810
Coordinates   2556294..2558030 (-) Length   578 a.a.
NCBI ID   WP_005501371.1    Uniprot ID   A0A377HIH9
Organism   Grimontia hollisae strain 1078-81     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2551294..2563030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY15_RS11720 - 2551725..2551937 (+) 213 WP_158161972.1 DUF2970 domain-containing protein -
  GPY15_RS11725 dam 2551856..2552761 (-) 906 WP_005501354.1 adenine-specific DNA-methyltransferase -
  GPY15_RS11730 - 2552864..2554369 (-) 1506 WP_158162351.1 AAA family ATPase -
  GPY15_RS11735 aroB 2554400..2555476 (-) 1077 WP_158161974.1 3-dehydroquinate synthase -
  GPY15_RS11740 aroK 2555492..2556010 (-) 519 WP_040528019.1 shikimate kinase AroK -
  GPY15_RS11745 pilQ 2556294..2558030 (-) 1737 WP_005501371.1 type IV pilus secretin PilQ Machinery gene
  GPY15_RS11750 - 2558027..2558557 (-) 531 WP_005501373.1 pilus assembly protein PilP -
  GPY15_RS11755 - 2558554..2559147 (-) 594 WP_005501375.1 type 4a pilus biogenesis protein PilO -
  GPY15_RS11760 - 2559137..2559727 (-) 591 WP_040528021.1 PilN domain-containing protein -
  GPY15_RS11765 pilM 2559717..2560652 (-) 936 WP_114996227.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62879.48 Da        Isoelectric Point: 4.6289

>NTDB_id=408193 GPY15_RS11745 WP_005501371.1 2556294..2558030(-) (pilQ) [Grimontia hollisae strain 1078-81]
MKSLRKGCQRQKLAMAGWVAAAIFAAPWALAENQLQQIDVTDGENGKTLLTLTLEDKSVVTNFNKTDTGLAISLKGTSVE
DNLVGVRSLRDVGKGTKNLAVKPIEDDVELLVTTSGHFGYDYYQTNNVLTVEVFPYASLPSSRNTSPKKGKRNKNISINF
QDIPVRSVLQMVAEHNGFNLVVSDTVQGSLTLRLDDVPWQKALDTILNVKGLDKRQTGNILLVAPKIELDEQERLVLEKR
RQARELASLRSEVIQIKYANAVDLKEMLGGSDAEEGGISLLSERGSIAVDPRTNSLVIKDLSDNIEVVKSLIDSLDIPVD
QVEIEARIVTVDEGTLDEIGVRWGLNSQNGNFTMGGSIEGNWQHSDRIDVGADNEPDELMLDDLLNVNLGAISPNATSIA
FSVANLGKDLLLDLELSALQAESRAEIISSPRLLTTNKRPAYIEQGTELPYLEASSSGAAAVSFKKAVLSLSVTPQITPD
NKLVLDLQVTQDKPAGTVKAGTGEAMAINTQRIGTQVLVNDGETVVLGGIYQHEMMEGVDKIPLLGDIPGLGQLFRRNYE
TTGKRELLIFVTPRVMTQ

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=408193 GPY15_RS11745 WP_005501371.1 2556294..2558030(-) (pilQ) [Grimontia hollisae strain 1078-81]
ATGAAAAGCCTGAGGAAAGGATGCCAACGCCAGAAGCTGGCCATGGCCGGATGGGTAGCTGCAGCTATCTTTGCCGCACC
ATGGGCACTGGCGGAGAACCAATTGCAACAAATTGACGTCACGGATGGGGAAAACGGTAAAACCCTGTTAACCCTGACGT
TAGAAGACAAGTCGGTAGTGACCAATTTTAATAAAACGGACACTGGCCTTGCTATCAGCCTTAAAGGCACGTCGGTAGAG
GATAACCTCGTTGGTGTCAGAAGCCTCAGGGATGTCGGGAAAGGCACAAAAAACCTGGCGGTTAAACCCATAGAAGACGA
TGTTGAGCTGTTAGTTACCACATCCGGTCACTTTGGCTATGACTATTACCAGACCAACAACGTCCTGACGGTAGAAGTTT
TTCCCTACGCCTCTTTACCATCCAGCAGGAATACCAGCCCGAAAAAAGGCAAACGTAATAAGAATATTTCCATTAATTTC
CAGGATATCCCTGTCCGTTCTGTACTGCAGATGGTCGCCGAACACAATGGCTTTAATCTGGTGGTCTCCGACACCGTTCA
GGGCAGCCTGACACTGCGCCTTGATGATGTGCCCTGGCAAAAAGCCCTGGATACTATTCTCAATGTGAAAGGACTGGATA
AACGCCAGACGGGTAACATCTTGTTGGTGGCCCCTAAAATCGAGCTTGATGAGCAAGAGCGTCTGGTTCTGGAAAAACGC
CGTCAGGCACGAGAGCTTGCGTCGCTGCGCTCGGAAGTTATCCAGATTAAGTATGCCAATGCGGTTGACCTTAAAGAGAT
GCTGGGTGGCAGTGATGCCGAAGAAGGGGGGATCAGCCTGCTGTCAGAGCGCGGCAGTATCGCCGTTGACCCACGGACTA
ACTCGCTGGTGATCAAAGACCTGTCTGACAATATCGAGGTGGTGAAATCGCTGATTGATTCCCTGGATATTCCGGTCGAT
CAGGTAGAAATTGAAGCGCGCATTGTAACAGTGGATGAAGGCACACTGGATGAAATTGGGGTGCGTTGGGGATTGAACAG
CCAAAATGGTAATTTCACCATGGGTGGGTCAATTGAAGGTAACTGGCAGCACTCAGATCGAATTGACGTTGGTGCGGACA
ATGAACCTGATGAGCTGATGCTGGATGACCTTTTGAATGTGAACTTAGGGGCTATCAGTCCGAATGCCACCAGTATCGCA
TTTTCTGTCGCCAATCTTGGTAAGGACCTGCTGCTGGATTTAGAGCTTTCTGCGCTACAAGCGGAATCACGTGCTGAGAT
TATTTCCAGCCCCCGCCTCCTGACCACCAACAAGCGCCCAGCCTACATTGAGCAGGGCACTGAGCTGCCGTACCTGGAAG
CCTCTTCCAGTGGCGCGGCGGCAGTGTCATTCAAAAAAGCGGTATTGAGCCTGTCGGTGACACCGCAAATCACCCCGGAC
AATAAGCTGGTGCTGGATCTTCAGGTAACCCAGGATAAACCCGCGGGCACGGTAAAAGCCGGAACCGGTGAGGCAATGGC
CATCAATACCCAACGCATCGGTACCCAGGTGCTGGTCAATGACGGAGAAACGGTGGTACTCGGTGGTATCTATCAACATG
AAATGATGGAAGGCGTCGATAAGATCCCGTTATTGGGGGATATTCCCGGGCTGGGGCAGCTCTTTAGACGAAACTATGAA
ACCACTGGCAAACGGGAACTGCTCATCTTTGTTACTCCGAGAGTAATGACTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(418-576)

Secretin and TonB N terminus short domain (STN)

(178-226)

Bacterial type II/III secretion system short domain (Secretin_N)

(252-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A377HIH9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

56.591

98.443

0.557
  pilQ Vibrio cholerae strain A1552

56.591

98.443

0.557
  pilQ Vibrio campbellii strain DS40M4

55.16

97.232

0.536
  pilQ Legionella pneumophila strain ERS1305867

41.058

94.81

0.389
  pilQ Legionella pneumophila strain Lp02

40.876

94.81

0.388
  comQ Acinetobacter baylyi ADP1

37.884

100

0.384
  pilQ Pseudomonas aeruginosa PAK

38.302

99.827

0.382

Multiple sequence alignment