Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPX90_RS10040 Genome accession   NZ_CP046752
Coordinates   1070338..1072029 (+) Length   563 a.a.
NCBI ID   WP_106223632.1    Uniprot ID   -
Organism   Photobacterium damselae strain 9046-81     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1065338..1077029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX90_RS10025 pdhR 1067619..1068392 (-) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  GPX90_RS10030 ampD 1068752..1069330 (-) 579 WP_005297607.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPX90_RS10035 - 1069822..1070337 (+) 516 WP_158135329.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPX90_RS10040 pilB 1070338..1072029 (+) 1692 WP_106223632.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPX90_RS10045 pilC 1072095..1073321 (+) 1227 WP_068969232.1 type II secretion system F family protein Machinery gene
  GPX90_RS10050 pilD 1073504..1074382 (+) 879 WP_151171377.1 A24 family peptidase Machinery gene
  GPX90_RS10055 coaE 1074384..1074986 (+) 603 WP_109374725.1 dephospho-CoA kinase -
  GPX90_RS10060 zapD 1075054..1075791 (+) 738 WP_005297594.1 cell division protein ZapD -
  GPX90_RS10065 yacG 1075800..1076009 (+) 210 WP_005297592.1 DNA gyrase inhibitor YacG -
  GPX90_RS10070 mutT 1076228..1076668 (+) 441 WP_158135331.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62494.09 Da        Isoelectric Point: 5.2594

>NTDB_id=407145 GPX90_RS10040 WP_106223632.1 1070338..1072029(+) (pilB) [Photobacterium damselae strain 9046-81]
MHTTLASILHQADLISSVQQQQVEDSVSMEGITVPSALIHLGIFSDRELAQHLSHIFGLTLVSVHDYDFEACCKAVYQRD
LVLRHRALPLAQNADTLFLGVSDPCNIDGQDEFRFATGRNIEPLLLENKQLESAIRRIYGRELGETTNMASRDVSDDDLN
NLVDIGDDELDQTTDLGQENEPVTRYINQVLLDAVRKKASDIHFEPYETSYRIRFRCDGILHQHATPPAHLSRRLSTRLK
VMSRLNIAERRQPQDGRIKLKLTANLAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDILGYNQQQKAAYLNALQQPQGM
ILMTGPTGSGKTVSLYTGLSILNTEERNISTAEDPVEINLPGINQVHINNQAGLGFAEALRSFLRQDPDVVMVGEIRDLE
TASIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIEDFNLASSLTLIIAQRLGRRLCPHCKQVDEIDGFMQDKLRIPAG
TTLYKANPEGCDDCNKGYLGRVGIYEVMPFSRRLAQALVDGATTLELENIAVEEGMLTLQQSGIEKLCLGITSLKELQRV
LHF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=407145 GPX90_RS10040 WP_106223632.1 1070338..1072029(+) (pilB) [Photobacterium damselae strain 9046-81]
ATGCACACCACTCTCGCCTCGATTCTGCACCAGGCTGATCTGATCAGCTCGGTGCAGCAGCAACAAGTCGAAGACAGTGT
CAGTATGGAGGGGATCACGGTTCCCTCCGCACTGATCCATCTGGGCATCTTTAGCGATCGCGAACTGGCCCAACACCTTA
GCCACATTTTTGGTCTCACCCTCGTTTCAGTCCATGACTACGATTTTGAAGCTTGCTGTAAAGCGGTCTATCAACGCGAT
CTGGTATTACGTCATCGTGCACTGCCCCTTGCTCAAAATGCGGACACACTGTTTTTAGGGGTCAGCGATCCATGCAATAT
CGACGGACAAGATGAGTTCCGTTTTGCCACTGGCCGTAATATTGAACCGCTACTGCTAGAAAACAAACAGCTCGAATCGG
CTATCCGTCGTATTTATGGCCGCGAGTTGGGCGAAACCACCAACATGGCCAGTCGCGATGTCAGTGATGATGATCTAAAT
AACTTAGTCGATATTGGTGATGATGAGCTGGATCAAACCACAGATCTAGGCCAAGAAAACGAACCTGTTACTCGCTATAT
TAATCAAGTGCTGCTCGATGCAGTACGTAAAAAAGCCTCCGATATCCATTTTGAACCATATGAGACCAGCTATCGGATCC
GCTTTCGTTGTGATGGGATCTTACACCAACACGCAACACCACCAGCCCATCTTTCTCGTCGTCTTTCTACCCGACTCAAA
GTGATGTCGCGCCTAAATATTGCCGAACGACGTCAACCGCAAGATGGACGCATAAAACTCAAGCTCACAGCCAATTTAGC
CGTAGATCTCCGTGTTTCTACCCTGCCCACTTTATGGGGGGAAAAAGTGGTGCTGCGTATTTTAGACAGCTCCAGTGCCA
ACTTAGACATTGATATTTTAGGTTACAACCAGCAACAAAAAGCCGCTTATCTTAATGCTCTGCAACAACCGCAAGGGATG
ATCTTAATGACAGGGCCTACGGGTAGCGGCAAAACCGTTTCGCTTTATACCGGCCTTAGCATTTTAAATACCGAAGAGCG
CAATATTTCTACCGCAGAAGATCCAGTGGAGATCAACTTACCTGGCATTAATCAGGTTCACATTAACAATCAAGCAGGAC
TGGGGTTTGCTGAAGCGCTGCGCTCATTTTTACGTCAAGACCCCGATGTGGTGATGGTAGGTGAAATCCGCGATCTGGAA
ACCGCCTCTATTGCTGTTAAAGCTGCGCAAACTGGGCACTTAGTGCTATCGACCTTGCACACCAATTCTGCCGCCGAAAC
CATTACCCGTTTAACCAATATGGGCATTGAAGACTTTAACCTAGCCTCATCGCTAACACTGATTATTGCTCAGCGCTTAG
GTCGCCGGCTCTGCCCTCACTGTAAACAAGTAGATGAAATTGACGGCTTTATGCAAGACAAACTGCGTATTCCGGCTGGC
ACTACCTTGTATAAAGCCAATCCAGAAGGGTGCGATGACTGCAATAAAGGCTATTTAGGTCGTGTCGGTATCTATGAAGT
AATGCCATTTAGTCGTCGGCTGGCTCAAGCTCTAGTTGATGGCGCGACTACCTTAGAGCTGGAAAATATCGCAGTAGAAG
AAGGGATGCTAACGCTACAACAATCGGGAATAGAAAAGCTCTGTCTTGGCATCACCAGTTTAAAAGAATTACAGCGCGTC
CTGCATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

61.989

100

0.62
  pilB Vibrio parahaemolyticus RIMD 2210633

61.101

100

0.611
  pilB Vibrio campbellii strain DS40M4

60.568

100

0.606
  pilB Legionella pneumophila strain ERS1305867

50.184

96.625

0.485
  pilB Acinetobacter baylyi ADP1

48.905

97.336

0.476
  pilB Acinetobacter baumannii D1279779

50.864

92.54

0.471
  pilF Neisseria gonorrhoeae MS11

44.366

100

0.448
  pilB/pilB1 Synechocystis sp. PCC 6803

39.692

92.185

0.366

Multiple sequence alignment