Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GO998_RS14515 Genome accession   NZ_CP046729
Coordinates   3082695..3083216 (-) Length   173 a.a.
NCBI ID   WP_075465282.1    Uniprot ID   -
Organism   Ralstonia syzygii strain LLRS-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3077695..3088216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO998_RS14495 (GO998_14510) - 3077780..3079468 (-) 1689 WP_211903963.1 tetratricopeptide repeat protein -
  GO998_RS14500 (GO998_14515) moaC 3079563..3080039 (+) 477 WP_075465279.1 cyclic pyranopterin monophosphate synthase MoaC -
  GO998_RS14505 (GO998_14520) - 3080113..3081849 (-) 1737 WP_075465280.1 PglL family O-oligosaccharyltransferase -
  GO998_RS14510 (GO998_14525) pilA 3081926..3082432 (-) 507 WP_211903964.1 pilin Machinery gene
  GO998_RS14515 (GO998_14530) pilA 3082695..3083216 (-) 522 WP_075465282.1 pilin Machinery gene
  GO998_RS14520 (GO998_14535) - 3083415..3084113 (-) 699 WP_211903965.1 TerC family protein -
  GO998_RS14525 (GO998_14540) sucD 3084268..3085149 (-) 882 WP_013213430.1 succinate--CoA ligase subunit alpha -
  GO998_RS14530 (GO998_14545) sucC 3085234..3086400 (-) 1167 WP_211903966.1 ADP-forming succinate--CoA ligase subunit beta -
  GO998_RS14535 (GO998_14550) - 3086468..3087094 (-) 627 WP_211903967.1 DUF2889 domain-containing protein -
  GO998_RS14540 (GO998_14555) recX 3087367..3087846 (-) 480 WP_211903968.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17761.45 Da        Isoelectric Point: 10.2418

>NTDB_id=406779 GO998_RS14515 WP_075465282.1 3082695..3083216(-) (pilA) [Ralstonia syzygii strain LLRS-1]
MASKHSRNRRRGQRGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLSLAAQAKALVVENAANAQSSLALGSASL
PASRNVSALNIDSSTGEITVVYASAVTTSGANTLVLTPYSGSSSALANLQAGSAPPSMVLWVCAAAGKSMPVTTVTQKTT
ATLPPKYAPAECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=406779 GO998_RS14515 WP_075465282.1 3082695..3083216(-) (pilA) [Ralstonia syzygii strain LLRS-1]
ATGGCATCGAAGCACAGCAGGAACAGGCGGCGCGGCCAGCGCGGCTTTACATTGATCGAGTTGATGATCGTGGTGGCGAT
CATCGGGATATTGGCGGCGATTGCGATTCCGGCGTACCAGGACTACACGATCCGCGCGCGCGTGACCGAAGGTCTGTCGC
TGGCTGCGCAGGCCAAGGCGCTGGTGGTTGAGAATGCCGCCAATGCCCAGAGCAGCTTGGCACTCGGCTCCGCATCCCTG
CCGGCGAGCAGGAATGTCAGTGCTTTGAATATTGATTCGTCTACGGGCGAGATTACTGTGGTCTACGCGTCTGCTGTCAC
GACCAGCGGGGCGAACACGCTGGTCTTGACCCCATACTCCGGAAGTTCGTCGGCGCTGGCTAATTTGCAGGCTGGCTCGG
CACCGCCGTCAATGGTGCTGTGGGTGTGCGCTGCCGCCGGAAAGTCGATGCCGGTCACCACCGTCACCCAGAAAACGACG
GCTACGTTGCCACCGAAATACGCGCCCGCGGAGTGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(45-173)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

54.545

100

0.555
  pilA2 Legionella pneumophila str. Paris

47.879

95.376

0.457
  pilA2 Legionella pneumophila strain ERS1305867

47.273

95.376

0.451
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.508

100

0.399
  comP Acinetobacter baylyi ADP1

42.073

94.798

0.399

Multiple sequence alignment