Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GO998_RS03030 Genome accession   NZ_CP046729
Coordinates   641081..642808 (-) Length   575 a.a.
NCBI ID   WP_211904481.1    Uniprot ID   -
Organism   Ralstonia syzygii strain LLRS-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 636081..647808
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO998_RS03000 (GO998_03000) - 636703..637164 (+) 462 WP_211904478.1 NUDIX domain-containing protein -
  GO998_RS03005 (GO998_03005) - 637157..637372 (-) 216 WP_013211401.1 DNA gyrase inhibitor YacG -
  GO998_RS03010 (GO998_03010) zapD 637384..638142 (-) 759 WP_013211402.1 cell division protein ZapD -
  GO998_RS03015 (GO998_03015) coaE 638260..638874 (-) 615 WP_211904479.1 dephospho-CoA kinase -
  GO998_RS03020 (GO998_03020) pilD 638887..639768 (-) 882 WP_283235487.1 prepilin peptidase Machinery gene
  GO998_RS03025 (GO998_03025) - 639771..641036 (-) 1266 WP_075463375.1 type II secretion system F family protein -
  GO998_RS03030 (GO998_03030) pilF 641081..642808 (-) 1728 WP_211904481.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GO998_RS03035 (GO998_03035) - 643056..644333 (-) 1278 WP_211904482.1 HlyC/CorC family transporter -
  GO998_RS03045 (GO998_03045) ispB 644612..645580 (-) 969 WP_211905030.1 octaprenyl diphosphate synthase -
  GO998_RS03050 (GO998_03050) rplU 645859..646170 (+) 312 WP_003271629.1 50S ribosomal protein L21 -
  GO998_RS03055 (GO998_03055) rpmA 646208..646468 (+) 261 WP_004633021.1 50S ribosomal protein L27 -
  GO998_RS03060 (GO998_03060) obgE 646655..647755 (+) 1101 WP_013211409.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63730.52 Da        Isoelectric Point: 7.4491

>NTDB_id=406767 GO998_RS03030 WP_211904481.1 641081..642808(-) (pilF) [Ralstonia syzygii strain LLRS-1]
MTLGLALAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDAVPAVLSANRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPIEELRQKMNVAIETVIVEHDKLVRQLSLANEAPTEIKSLIPKQEATQIEYDPG
AAAAVRRTTTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILSQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKETIERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPRETL
LEAGFKEADLDGSWQPYHPVGCETCNGSGYKGRVGIYQVMPITEAIQEIILTHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=406767 GO998_RS03030 WP_211904481.1 641081..642808(-) (pilF) [Ralstonia syzygii strain LLRS-1]
ATGACTCTCGGACTCGCTCTTGCGCAAAGCCGGCGAATTGCGCCGACATTACTCACCCAGCTTGAACAAAGCGCGCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCGCACGATCTCGCCGTCTTCGTGGCAGGCA
AGTACCAGATGCCGCTGCTGGACCTGGCGCAGTACAAGCTCGATGCCGTGCCGGCCGTGCTGTCGGCCAACAGGCATTTC
CAGCAATTGCGCCTGTTGCCGCTGGGCCGGCGCGACAACCGCATCATCGTTGCCACCTCGGACCCGAGCGACCCCAAGCC
GATCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCGCCAACGAAGCGCCGACCGAGATCAAGTCGCTCATCCCCAAGCAGGAAGCCACGCAGATCGAATACGACCCGGGC
GCAGCCGCTGCGGTGCGGCGCACCACGACCGACGGCATCGACGATGCGCCGGTGGTGCGTTTCCTGCAAAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACATTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCTCGCAGGTCGCGCAGCCGCCGCTGGATATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATCTCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACGGTCCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGACGATCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCCACCGAGCGGCTCGATATCGACCAGCTAGGCTATGAGCCCGAGCAAAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTCTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGTTGCCCGGCATCAACCAAG
TCAACGTCAACGACAAAGCGGGGCTGACCTTCGCCGCGGCGCTGAAATCGTTCCTGCGGCAGGATCCGGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGATGCGCCGACCACGCTCACGCGACTGATGAACATGGGCGTGGCACCGTTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCATGCAAGAAGCCGGGCCCGCTGCCCCGGGAAACACTG
CTGGAGGCCGGCTTCAAGGAAGCCGACCTGGACGGTTCGTGGCAGCCGTACCATCCAGTCGGCTGCGAGACATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCACCCACG
GCACGGCACTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCTGGCCTGCGGAAAGTGAAA
CAGGGGCTGACTTCCCTTGAGGAAGTGCTGGCCACGACGAACCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.333

93.913

0.501
  pilB Acinetobacter baumannii D1279779

50.973

98.261

0.501
  pilB Acinetobacter baylyi ADP1

49.911

97.913

0.489
  pilB Legionella pneumophila strain ERS1305867

46.235

99.304

0.459
  pilB Vibrio cholerae strain A1552

44.784

96.696

0.433
  pilB Vibrio campbellii strain DS40M4

44.834

94.261

0.423
  pilB Vibrio parahaemolyticus RIMD 2210633

44.649

94.261

0.421
  pilF Thermus thermophilus HB27

37.699

98.261

0.37

Multiple sequence alignment