Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GO593_RS08160 Genome accession   NZ_CP046654
Coordinates   1710837..1712549 (-) Length   570 a.a.
NCBI ID   WP_001274985.1    Uniprot ID   D0CAX6
Organism   Acinetobacter baumannii strain ATCC 19606     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1705837..1717549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO593_RS08130 (GO593_08130) - 1705999..1706325 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  GO593_RS08135 (GO593_08135) rlmB 1706427..1707176 (+) 750 WP_001118149.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GO593_RS08140 (GO593_08140) - 1707212..1708126 (+) 915 WP_000075011.1 DMT family transporter -
  GO593_RS08145 (GO593_08145) coaE 1708123..1708719 (-) 597 WP_000874125.1 dephospho-CoA kinase -
  GO593_RS19315 (GO593_08150) pilD 1708721..1709581 (-) 861 WP_001152283.1 prepilin peptidase Machinery gene
  GO593_RS08155 (GO593_08155) pilC 1709581..1710807 (-) 1227 WP_000279216.1 type II secretion system F family protein Machinery gene
  GO593_RS08160 (GO593_08160) pilB 1710837..1712549 (-) 1713 WP_001274985.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GO593_RS08165 (GO593_08165) tpiA 1712840..1713634 (+) 795 WP_000016932.1 triose-phosphate isomerase -
  GO593_RS08170 (GO593_08170) secG 1713647..1713976 (+) 330 WP_000555569.1 preprotein translocase subunit SecG -
  GO593_RS08185 (GO593_08185) rimP 1714452..1714976 (+) 525 WP_000777730.1 ribosome maturation factor RimP -
  GO593_RS08190 (GO593_08190) nusA 1715014..1716498 (+) 1485 WP_000532248.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63341.69 Da        Isoelectric Point: 5.2371

>NTDB_id=406488 GO593_RS08160 WP_001274985.1 1710837..1712549(-) (pilB) [Acinetobacter baumannii strain ATCC 19606]
MSALHTSPKFTGFFRRLVEEKHVSAATMQTALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVNLDGPTAQEDEDEAPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=406488 GO593_RS08160 WP_001274985.1 1710837..1712549(-) (pilB) [Acinetobacter baumannii strain ATCC 19606]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGACGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAACTGCATTAGATGCAGCGAAAAGAGCTAAGCAGGACACAGTCGCATATTTAATTGAAGAGGTTCATCTCTCCC
CTTCATTATTGGCTGAAACAATTTCTGCTGAGTTTGCTGAACCATATTTCGATCTGGATGTTTATGACACTTCTCAGATT
CCTAAAGATCTGGTCGATCAGAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGGCAAATTTTATATGT
CGCGACCAGTAATCCAAGCAATATCGAAGCGATTGATGCAATTCGTTTTAATAGTAAATTGCTGGTTGAGCCCGTAATTG
TGGAGCACCATAAACTCGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTCAATGATGAAGAGTTT
GATCTCGATGTTAATCTTGATGGCCCTACCGCTCAAGAAGATGAAGATGAAGCTCCTCAAGGCGATGAAGCCCCAATTGT
TAAATATATTAATAAGTTATTAATTGATGCCATTCGTATGGGTGCTTCAGACTTACATTTTGAACCGTACGAAAAATCCT
ATCGGGTGCGCTATCGTGTCGATGGCGTGTTACGTCAAATTGCTAATCCCCCTCTACAGCTTGCAAACCGCTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGTGTCCCACAAGATGGTCGTATCAAGCTCAAGCTGTCAAA
AAGCAAAGCGATTGATTTTCGTGTGAACTCCCTCCCGACTTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGAATTGATGCACTAGGATATGAAGAAGATCAAAAAGCTTTGTTTATGGAGGCATTAGATAAACCT
CAAGGCATGCTTTTAATTACCGGACCAACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTTTAAATATTTTAAATAC
TGAAAGCTCCAATATTTCTACTGCCGAAGACCCGGTAGAAATTAACCTTGAAGGAATTAATCAGGTTAACGTAAACCCCA
AGGTAGGGCTTACTTTTGCGGCCGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCGGCTCAAACTGGCCACATGGTGATGTCAACACTACATACCAATAGTGC
TCCGGAAACATTGACCCGTCTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAACCTAGTTATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGCAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCGCACCCAGATTTTCGGGTTTTTCAGCCCGTAGGTTGCCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCAGCTGCTTCTGAGAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTCAACCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D0CAX6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

99.649

100

0.996
  pilB Acinetobacter baylyi ADP1

79.649

100

0.796
  pilB Legionella pneumophila strain ERS1305867

50.97

99.474

0.507
  pilF Neisseria gonorrhoeae MS11

50.178

98.596

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

51.698

92.982

0.481
  pilB Vibrio campbellii strain DS40M4

50.482

91.053

0.46
  pilB Vibrio cholerae strain A1552

50.289

91.053

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.342

100

0.381
  pilF Thermus thermophilus HB27

36.833

98.596

0.363

Multiple sequence alignment