Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   UMN179_RS09180 Genome accession   NC_015460
Coordinates   1959936..1961345 (+) Length   469 a.a.
NCBI ID   WP_013746606.1    Uniprot ID   F4HD70
Organism   Gallibacterium anatis UMN179     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1954936..1966345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UMN179_RS09155 (UMN179_01815) dusA 1955913..1956893 (-) 981 WP_013746601.1 tRNA dihydrouridine(20/20a) synthase DusA -
  UMN179_RS09160 (UMN179_01816) - 1956874..1958268 (-) 1395 WP_013746602.1 chloride channel protein -
  UMN179_RS09165 (UMN179_01817) - 1958377..1958712 (-) 336 WP_013746603.1 hypothetical protein -
  UMN179_RS09170 (UMN179_01818) ampD 1958799..1959353 (-) 555 WP_013746604.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  UMN179_RS09175 (UMN179_01819) pilA 1959458..1959919 (+) 462 WP_013746605.1 prepilin peptidase-dependent pilin Machinery gene
  UMN179_RS09180 (UMN179_01820) pilB 1959936..1961345 (+) 1410 WP_013746606.1 GspE/PulE family protein Machinery gene
  UMN179_RS09185 (UMN179_01821) - 1961361..1962569 (+) 1209 WP_013746607.1 type II secretion system F family protein -
  UMN179_RS09190 (UMN179_01822) - 1962566..1963210 (+) 645 WP_237699461.1 prepilin peptidase -
  UMN179_RS13525 (UMN179_01823) - 1963271..1963407 (-) 137 Protein_1800 transposase -
  UMN179_RS09195 (UMN179_01824) - 1963475..1964611 (+) 1137 WP_043885282.1 RNA-guided endonuclease InsQ/TnpB family protein -
  UMN179_RS09200 (UMN179_01825) coaE 1964777..1965409 (+) 633 WP_013746610.1 dephospho-CoA kinase -
  UMN179_RS09205 (UMN179_01826) yacG 1965399..1965617 (+) 219 WP_013746611.1 DNA gyrase inhibitor YacG -
  UMN179_RS09210 (UMN179_01827) - 1965704..1965976 (+) 273 WP_013746612.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52858.32 Da        Isoelectric Point: 5.3117

>NTDB_id=40624 UMN179_RS09180 WP_013746606.1 1959936..1961345(+) (pilB) [Gallibacterium anatis UMN179]
MGYCVKALDSEQLFEISELQWQKNQQQYELLLRYLAVPLQEQAQQLWLAVESFDNLAACETFAFLSGKQIEPVAIGQTAL
KQLLQQLQPGTATNQVQESQLHSYQQNDIVAKSDEIAEVDAQEPIIQLFNQLLESGLQYSVSDIHLEPLRQEFQIRFRID
GVLQRHQVLTLSIGQRLNSRLKLLAKLDINELRLPQDGRFRFKTAFGETLDIRLSSLPTQYGEKIVLRLQPNTPVFDDFT
ELGMTNEQAVLFRQALSQPQGLILVTGPTGSGKSLTLYSGLHFLNQESRHILTAEDPIELQLNGIIQTQANAAIGLTFAT
LLRSFLRQDPDVIMVGEIRDQETAEIALRAAQTGHLVLSTLHTNTAIGAISRLQQLGIAQHELENSLLLVVAQRLVRKRC
TLCQGDKSQQCDCVDGYRGRIGVYQLLHYQQQQIQMDYPTLLAAAQLKLQQRITTTAEVERVFGKTENS

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=40624 UMN179_RS09180 WP_013746606.1 1959936..1961345(+) (pilB) [Gallibacterium anatis UMN179]
ATGGGCTATTGTGTAAAGGCATTGGATTCGGAGCAGCTGTTTGAAATTTCAGAATTGCAATGGCAGAAGAATCAACAGCA
ATATGAACTGTTATTGCGTTATTTAGCTGTACCGTTGCAGGAACAAGCGCAACAGTTATGGTTAGCGGTTGAGTCTTTCG
ATAATTTAGCAGCTTGCGAGACCTTTGCTTTTCTTTCCGGCAAACAGATTGAGCCTGTTGCGATCGGACAAACTGCACTG
AAACAGTTATTACAGCAGCTGCAACCCGGAACGGCGACAAATCAAGTGCAAGAATCACAACTGCATAGTTATCAGCAAAA
TGATATTGTCGCAAAATCGGATGAAATTGCGGAAGTTGATGCACAAGAGCCGATTATTCAACTATTCAATCAATTATTGG
AATCAGGTTTGCAATATTCCGTTTCTGATATTCATCTTGAGCCGTTACGTCAGGAGTTCCAGATCCGCTTTAGAATTGAC
GGCGTATTACAACGGCATCAGGTGCTGACATTGTCAATAGGTCAACGCTTAAACTCACGCCTAAAATTGTTGGCAAAACT
GGATATTAACGAATTACGCTTGCCGCAGGATGGTCGTTTTCGTTTTAAAACCGCATTCGGTGAAACATTAGATATTCGGT
TATCTTCTCTACCGACACAATATGGTGAAAAAATCGTATTACGATTGCAACCTAACACACCGGTGTTTGATGATTTTACC
GAACTGGGAATGACAAACGAGCAGGCTGTGCTGTTTCGGCAAGCGTTATCTCAACCGCAAGGATTAATTTTGGTGACCGG
ACCGACCGGAAGTGGTAAAAGTTTGACATTATATAGCGGTTTACATTTTTTAAATCAGGAAAGTCGCCATATTCTTACAG
CGGAAGATCCGATAGAACTGCAATTAAACGGTATTATTCAGACCCAAGCAAATGCCGCAATCGGTTTAACTTTTGCAACA
TTATTGCGGAGTTTTTTACGCCAAGATCCGGATGTCATTATGGTTGGGGAAATTCGAGATCAGGAAACGGCAGAGATAGC
ATTACGCGCGGCACAGACGGGACATTTAGTGTTATCGACACTGCATACCAATACGGCAATCGGTGCAATTTCCCGATTGC
AGCAGTTGGGAATAGCACAACATGAATTGGAAAATTCATTATTGCTGGTGGTGGCACAACGTTTAGTCCGCAAACGTTGC
ACCTTATGCCAAGGTGATAAATCTCAGCAATGCGATTGTGTTGACGGTTATCGCGGACGAATCGGCGTTTATCAACTGTT
ACATTATCAGCAACAACAAATTCAAATGGATTATCCGACATTATTGGCGGCAGCACAGCTGAAATTACAGCAACGGATCA
CGACAACAGCAGAGGTTGAACGCGTCTTCGGCAAGACGGAGAACAGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-91)

Type II/IV secretion system protein (T2SSE)

(131-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F4HD70

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

59.649

97.228

0.58
  pilB Haemophilus influenzae Rd KW20

59.649

97.228

0.58
  pilB Glaesserella parasuis strain SC1401

53.846

99.787

0.537
  pilB Vibrio parahaemolyticus RIMD 2210633

38.477

100

0.409
  pilB Vibrio cholerae strain A1552

39.834

100

0.409
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.405
  pilB Legionella pneumophila strain ERS1305867

36.157

100

0.373
  pilB Acinetobacter baylyi ADP1

39.503

94.456

0.373
  pilB Acinetobacter baumannii D1279779

39.13

93.177

0.365

Multiple sequence alignment