Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   UMN179_RS09175 Genome accession   NC_015460
Coordinates   1959458..1959919 (+) Length   153 a.a.
NCBI ID   WP_013746605.1    Uniprot ID   F4HD69
Organism   Gallibacterium anatis UMN179     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1954458..1964919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UMN179_RS09155 (UMN179_01815) dusA 1955913..1956893 (-) 981 WP_013746601.1 tRNA dihydrouridine(20/20a) synthase DusA -
  UMN179_RS09160 (UMN179_01816) - 1956874..1958268 (-) 1395 WP_013746602.1 chloride channel protein -
  UMN179_RS09165 (UMN179_01817) - 1958377..1958712 (-) 336 WP_013746603.1 hypothetical protein -
  UMN179_RS09170 (UMN179_01818) ampD 1958799..1959353 (-) 555 WP_013746604.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  UMN179_RS09175 (UMN179_01819) pilA 1959458..1959919 (+) 462 WP_013746605.1 prepilin peptidase-dependent pilin Machinery gene
  UMN179_RS09180 (UMN179_01820) pilB 1959936..1961345 (+) 1410 WP_013746606.1 GspE/PulE family protein Machinery gene
  UMN179_RS09185 (UMN179_01821) - 1961361..1962569 (+) 1209 WP_013746607.1 type II secretion system F family protein -
  UMN179_RS09190 (UMN179_01822) - 1962566..1963210 (+) 645 WP_237699461.1 prepilin peptidase -
  UMN179_RS13525 (UMN179_01823) - 1963271..1963407 (-) 137 Protein_1800 transposase -
  UMN179_RS09195 (UMN179_01824) - 1963475..1964611 (+) 1137 WP_043885282.1 RNA-guided endonuclease InsQ/TnpB family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 16390.72 Da        Isoelectric Point: 8.0868

>NTDB_id=40623 UMN179_RS09175 WP_013746605.1 1959458..1959919(+) (pilA) [Gallibacterium anatis UMN179]
MKKREQLKSNYAGFTLIELMIVIAIIAVLATIAIPSYQNYTKKAAISELIQASSPYKAEVELCIYNTKSKASCNAGQNGI
QAKPSNMDKFKYLKEVNVAAGVISVSGKSSLENISYTMTPSDGNNGELIDWSVTCSGDNGLFPAGFCSNTSAN

Nucleotide


Download         Length: 462 bp        

>NTDB_id=40623 UMN179_RS09175 WP_013746605.1 1959458..1959919(+) (pilA) [Gallibacterium anatis UMN179]
ATGAAAAAACGTGAACAACTTAAGTCGAATTATGCCGGATTTACTTTAATCGAATTGATGATCGTCATTGCGATTATTGC
CGTGTTGGCAACCATTGCCATTCCCTCTTATCAAAATTACACCAAAAAGGCGGCAATATCAGAATTGATTCAGGCCTCTT
CACCCTATAAGGCGGAAGTTGAACTCTGTATTTATAACACTAAGAGTAAAGCAAGCTGTAACGCCGGTCAAAACGGCATT
CAGGCGAAGCCGTCTAATATGGATAAATTTAAATATCTTAAAGAGGTTAATGTGGCTGCCGGTGTTATTTCTGTTTCCGG
TAAATCCAGTCTGGAGAACATCAGTTATACGATGACACCAAGTGACGGCAATAACGGTGAATTAATTGATTGGAGTGTAA
CTTGTAGCGGTGATAATGGGCTGTTTCCGGCAGGATTCTGTAGCAATACTTCTGCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(9-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F4HD69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

54

98.039

0.529
  pilA Haemophilus influenzae Rd KW20

52.667

98.039

0.516
  pilA Haemophilus influenzae 86-028NP

56.618

88.889

0.503

Multiple sequence alignment