Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GOQ09_RS21515 Genome accession   NZ_CP046622
Coordinates   4602655..4604394 (+) Length   579 a.a.
NCBI ID   WP_157615542.1    Uniprot ID   A0A6I6HM03
Organism   Variovorax paradoxus strain CSUSB     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4597655..4609394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GOQ09_RS21480 (GOQ09_21470) - 4597696..4598214 (+) 519 WP_242630906.1 CNP1-like family protein -
  GOQ09_RS21485 (GOQ09_21475) proB 4598247..4599386 (-) 1140 WP_157615538.1 glutamate 5-kinase -
  GOQ09_RS21490 (GOQ09_21480) cgtA 4599498..4600574 (-) 1077 WP_126746974.1 Obg family GTPase CgtA -
  GOQ09_RS21495 (GOQ09_21485) rpmA 4600645..4600905 (-) 261 WP_028259150.1 50S ribosomal protein L27 -
  GOQ09_RS21500 (GOQ09_21490) rplU 4600918..4601229 (-) 312 WP_157615540.1 50S ribosomal protein L21 -
  GOQ09_RS21505 (GOQ09_21495) - 4601421..4602413 (+) 993 WP_157616804.1 polyprenyl synthetase family protein -
  GOQ09_RS21515 (GOQ09_21505) pilB 4602655..4604394 (+) 1740 WP_157615542.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GOQ09_RS21520 (GOQ09_21510) pilC 4604424..4605653 (+) 1230 WP_157615544.1 type II secretion system F family protein Machinery gene
  GOQ09_RS21525 (GOQ09_21515) - 4605653..4606618 (+) 966 WP_157615546.1 A24 family peptidase -
  GOQ09_RS21530 (GOQ09_21520) coaE 4606629..4607231 (+) 603 WP_157615549.1 dephospho-CoA kinase -
  GOQ09_RS21535 (GOQ09_21525) - 4607207..4608799 (+) 1593 WP_242630907.1 MBOAT family protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62780.98 Da        Isoelectric Point: 5.7633

>NTDB_id=406118 GOQ09_RS21515 WP_157615542.1 4602655..4604394(+) (pilB) [Variovorax paradoxus strain CSUSB]
MAAAELPVKENTQIALPGLARALVSAGKLPAKTAEDIYQKSLSSRTSFIAELTGSGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCLAYRIVVLSKRNNRLIVATADPSDQQAVEKIKFASQMGVDWVIAEYDKLSRMIEAAAVSASETL
NNIVGGGDFEFDDVTADTSGDTNEQAAIAEVEDAPVVRFLHKMLLDGVSMRASDIHFEPYEHNYRVRFRIDGELREIASP
PTLIKDKLASRIKVISRLDISEKRIPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYDADEK
ERLLSAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAAALRAFLRQD
PDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCHLCRAPADV
PRQALLDAGFKEEELDGSWKPYRPIGCSACNGGYKGRVGIYQVMPISEAIQAIILRDGSALDIARQSEAEGVRSLRQSGL
RKVMQGLTSLEEVVAVTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=406118 GOQ09_RS21515 WP_157615542.1 4602655..4604394(+) (pilB) [Variovorax paradoxus strain CSUSB]
ATGGCTGCCGCCGAACTTCCTGTTAAAGAAAACACGCAAATCGCCCTTCCGGGCCTTGCGCGCGCCTTGGTTTCGGCCGG
CAAGCTTCCTGCAAAAACAGCGGAAGACATCTACCAGAAGTCGCTGAGCAGCCGGACCAGCTTCATTGCAGAGCTGACCG
GCAGCGGCGCCGTCTCGGCCGCCGACCTGGCCCACACGCTTTCTTCCGCCTTTGGCGCTCCGCTGCTCGACCTGGACGCG
ATCGACCACCAGCGGCTGCCCAAGGACCTGCTCGACCCGAAGCTGTGCCTTGCATACCGCATCGTGGTGCTCAGCAAGCG
CAACAACCGTCTCATCGTTGCGACAGCGGACCCTTCCGATCAGCAGGCCGTGGAAAAGATCAAGTTCGCGTCCCAGATGG
GCGTGGACTGGGTCATTGCCGAGTACGACAAGCTGTCGCGCATGATCGAGGCCGCTGCTGTCAGCGCATCGGAAACCCTC
AACAATATCGTCGGCGGTGGGGATTTCGAGTTCGATGACGTCACGGCGGACACCTCGGGCGATACGAACGAACAGGCGGC
TATCGCCGAAGTCGAGGATGCCCCGGTCGTCCGGTTCCTGCACAAGATGCTGCTCGATGGCGTCAGCATGCGCGCATCGG
ACATTCACTTCGAGCCGTACGAGCACAACTACCGGGTGCGCTTTCGCATCGACGGCGAACTGCGCGAAATCGCCAGCCCG
CCGACGCTCATCAAGGACAAGTTGGCCTCTCGGATCAAGGTCATTTCCCGGCTCGATATCTCCGAAAAGCGCATCCCGCA
AGACGGGCGCATGAAGCTCAAGATCGGCCCGGATCGCGTGATCGATTTTCGGGTGAGCACTTTGCCCACGCTGTTCGGCG
AGAAGATCGTGATCCGTATCTTGGATCCGAGCAGCGCGCGGCTTGGCATCGATGCGCTTGGTTACGACGCGGACGAAAAA
GAGCGGCTGCTGAGTGCGATCGGCCGTCCTTACGGCATGGTGCTGGTGACGGGCCCCACGGGTTCCGGAAAGACGGTGTC
GCTCTACACCTGCCTGAACCTGCTGAACCAGCCCGGCGTCAACATTGCCACGGCGGAAGATCCGTCCGAAATCAACCTGC
CCGGGGTGAACCAGGTCAACGTCAACGAACGCGCCGGACTCACCTTCGCCGCCGCGCTGCGCGCCTTTCTCCGGCAAGAT
CCCGACATCATCATGGTCGGTGAAATCCGGGACCTCGAAACAGCCGACATCTCGATCAAGGCCGCCCAAACGGGCCACCT
GGTGCTTTCGACGCTGCACACGAACGACGCGCCGACCACGCTCACACGCATGCGGAACATGGGCATCGCGCCGTTCAACA
TTGCCTCGAGCGTCATCCTGATCACTGCGCAACGCCTGGCTCGCCGCCTATGCCATCTCTGCCGCGCGCCGGCCGACGTG
CCGCGCCAGGCCCTGCTCGATGCGGGCTTCAAGGAAGAAGAGCTCGACGGCTCCTGGAAGCCCTATCGTCCGATCGGCTG
CTCCGCGTGCAACGGCGGCTACAAGGGCCGCGTCGGCATCTACCAGGTGATGCCGATCTCCGAAGCTATCCAAGCCATCA
TCCTGCGCGACGGCAGCGCGTTGGACATTGCGCGCCAGTCCGAAGCCGAGGGCGTTCGCTCGCTGCGGCAGTCCGGTCTC
AGAAAAGTCATGCAAGGGCTTACCTCACTCGAGGAAGTGGTGGCGGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I6HM03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.846

98.791

0.532
  pilB Acinetobacter baylyi ADP1

52.9

98.273

0.52
  pilF Neisseria gonorrhoeae MS11

52.212

97.582

0.509
  pilB Legionella pneumophila strain ERS1305867

49.381

97.582

0.482
  pilB Vibrio cholerae strain A1552

46.073

98.964

0.456
  pilB Vibrio parahaemolyticus RIMD 2210633

45.534

98.618

0.449
  pilB Vibrio campbellii strain DS40M4

45.053

97.755

0.44
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.414

91.883

0.371
  pilF Thermus thermophilus HB27

37.368

98.446

0.368

Multiple sequence alignment