Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   XaFJ1_RS03035 Genome accession   NZ_CP046570
Coordinates   670057..671784 (+) Length   575 a.a.
NCBI ID   WP_012915195.1    Uniprot ID   D2UBN4
Organism   Xanthomonas albilineans strain Xa-FJ1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 665057..676784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XaFJ1_RS03015 (XaFJ1_GM000631) coaE 666327..666950 (-) 624 WP_012915191.1 dephospho-CoA kinase -
  XaFJ1_RS03020 (XaFJ1_GM000632) - 666963..667826 (-) 864 WP_012915192.1 A24 family peptidase -
  XaFJ1_RS03025 (XaFJ1_GM000633) pilC 667833..669104 (-) 1272 WP_012915193.1 type II secretion system F family protein Machinery gene
  XaFJ1_RS03030 (XaFJ1_GM000634) - 669470..669907 (+) 438 WP_012915194.1 pilin -
  XaFJ1_RS03035 (XaFJ1_GM000635) pilB 670057..671784 (+) 1728 WP_012915195.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XaFJ1_RS03040 (XaFJ1_GM000636) pilR 671928..673316 (-) 1389 WP_012915196.1 sigma-54 dependent transcriptional regulator Regulator
  XaFJ1_RS03045 (XaFJ1_GM000637) - 673404..675017 (-) 1614 WP_012915197.1 ATP-binding protein -
  XaFJ1_RS03050 (XaFJ1_GM000638) sucC 675308..676471 (+) 1164 WP_012915198.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62509.65 Da        Isoelectric Point: 5.9527

>NTDB_id=405610 XaFJ1_RS03035 WP_012915195.1 670057..671784(+) (pilB) [Xanthomonas albilineans strain Xa-FJ1]
MSAISANLVGITGIARRLVQDGVLEEAAARAAMAQATEARVPLPQWFAEKKLVTAAQLAAANAVEFGVPLLDASVFDPSQ
NAMKLVSEELLHKHQVLPLFKRGSRLFVGISNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWHSSNDALGSALGG
DDEGMDNLDVGGDEDGGSGDTGMDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKNKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQQLFL
DAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPVKLPDKA
LLAEGFTEADIAAGIQLYEPVGCEECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIASAAQKIGVNDLRQSALLKAR
NGITSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=405610 XaFJ1_RS03035 WP_012915195.1 670057..671784(+) (pilB) [Xanthomonas albilineans strain Xa-FJ1]
ATGAGCGCCATATCAGCCAATCTCGTCGGCATCACCGGCATCGCCCGCCGTCTCGTTCAGGATGGGGTCTTGGAGGAGGC
CGCGGCGCGGGCCGCCATGGCCCAGGCGACCGAGGCCAGGGTCCCGCTGCCGCAATGGTTTGCCGAGAAGAAGCTGGTCA
CCGCTGCGCAGTTGGCCGCCGCCAATGCGGTGGAATTCGGCGTGCCATTGCTGGACGCCTCGGTGTTCGACCCCAGCCAG
AACGCGATGAAGCTGGTCAGCGAGGAACTGCTGCACAAGCACCAGGTGCTGCCGTTGTTCAAGCGCGGTAGCCGGCTGTT
CGTGGGGATCAGCAATCCCACCCAGACCCGCGCGCTGGACGACATCAAGTTCCATACCAATCTCACCGTCGAGCCGATCC
TGGTCGATGAGGACCAGATCCGGCGCACGCTGGAGCAGTGGCATTCCAGCAACGATGCGCTCGGCAGTGCGTTGGGCGGC
GACGACGAGGGCATGGACAACCTCGATGTGGGCGGCGACGAGGACGGCGGCAGCGGCGACACCGGTATGGACGCCAAGAG
CGACGACACGCCGGTGGTCAAGTTCGTCAACAAGGTGCTGATCGATGCGATCCGCCGCGGTGCCTCGGATATCCATTTCG
AGCCCTACGAGGACGACTACCGGGTGCGCCTGCGCATCGATGGCCTGCTCAAGAACGTGGCCAAGGCGCCGGTCAAGCTC
AATCAGCGCATCGCCGCGCGGCTGAAGGTGATGGCGCAGTTGGACATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGCAT
CAAGCTCAATCTGTCCAAGAACAAGCAGATCGACTTCCGCGTCAGCACACTGCCGACGCTGTTCGGCGAGAAGATCGTGC
TGCGTATTCTCGACGGCAGTGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAACAGCAGCAGTTGTTCCTG
GACGCGATCCATAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCCGGCAAGACGGTGTCGCTGTATACCGC
GCTGGGCATTCTCAACGACGAAACCCGCAACATTTCCACCGCCGAGGACCCGGTGGAAATCCGTCTGCCGGGCGTCAATC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCAGCCTTGCGTTCGTTCCTGCGGCAGGACCCTGACATCATC
ATGGTCGGCGAAATCCGCGACTTGGAAACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGTCACATGGTGCTGTCCAC
CCTGCACACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGCATCGCCCCCTACAACATCACCAGTTCGG
TGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGCTGTGCAACAACTGCAAACGCCCGGTCAAATTGCCGGATAAAGCC
TTGCTGGCCGAAGGCTTCACCGAAGCGGACATCGCCGCGGGGATCCAGTTGTACGAGCCGGTCGGTTGCGAGGAATGCAC
CGAAGGCTACAAGGGCCGCACCGGCATCTACCAGGTGATGCCGATGAACGACGAAATCGCCGCGATCGTGCTGCAGGGCG
GTAACGCGATGGACATCGCCAGCGCCGCGCAGAAGATCGGGGTCAATGATCTGCGTCAGTCGGCCTTACTCAAGGCGCGC
AATGGCATCACCAGTTTGGCTGAGATCAACCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D2UBN4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.382

100

0.555
  pilB Acinetobacter baylyi ADP1

56.007

98.435

0.551
  pilB Legionella pneumophila strain ERS1305867

51.408

98.783

0.508
  pilB Vibrio cholerae strain A1552

49.301

99.478

0.49
  pilF Neisseria gonorrhoeae MS11

48.858

98.957

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

46.986

98.087

0.461
  pilB Vibrio campbellii strain DS40M4

46.06

99.304

0.457
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.808

90.435

0.36

Multiple sequence alignment