Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GNX71_RS26655 Genome accession   NZ_CP046508
Coordinates   5740251..5741984 (+) Length   577 a.a.
NCBI ID   WP_206175203.1    Uniprot ID   -
Organism   Variovorax sp. RKNM96     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5735251..5746984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNX71_RS26625 (GNX71_26570) proB 5735881..5737020 (-) 1140 WP_206175200.1 glutamate 5-kinase -
  GNX71_RS26630 (GNX71_26575) cgtA 5737101..5738177 (-) 1077 WP_206175201.1 Obg family GTPase CgtA -
  GNX71_RS26635 (GNX71_26580) rpmA 5738249..5738509 (-) 261 WP_013543198.1 50S ribosomal protein L27 -
  GNX71_RS26640 (GNX71_26585) rplU 5738522..5738833 (-) 312 WP_042578903.1 50S ribosomal protein L21 -
  GNX71_RS26645 (GNX71_26590) - 5739017..5740009 (+) 993 WP_206175202.1 polyprenyl synthetase family protein -
  GNX71_RS26655 (GNX71_26600) pilB 5740251..5741984 (+) 1734 WP_206175203.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GNX71_RS26660 (GNX71_26605) pilC 5742009..5743241 (+) 1233 WP_206175204.1 type II secretion system F family protein Machinery gene
  GNX71_RS26665 (GNX71_26610) - 5743241..5744206 (+) 966 WP_206175205.1 A24 family peptidase -
  GNX71_RS26670 (GNX71_26615) coaE 5744217..5744825 (+) 609 WP_206175206.1 dephospho-CoA kinase -
  GNX71_RS26675 (GNX71_26620) - 5744899..5746392 (+) 1494 WP_206175207.1 MBOAT family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62411.75 Da        Isoelectric Point: 5.5869

>NTDB_id=404773 GNX71_RS26655 WP_206175203.1 5740251..5741984(+) (pilB) [Variovorax sp. RKNM96]
MAAAELPVKELSQIALPGLARALVSAGKLPAKIAEDIYQKSLSGRTSFIAELTGTGAVSAADLAHTLSTAFGAPLLDLDA
IDQQRLPKDLLDPKLCLAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAATAAETI
NSIVGAEFEFDDVGADTSGDASEQAVAEVEDAPVVRFLHKMLLDAVSMRASDIHFEPYEHHYRVRFRVDGELREIASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARLGIDALGYDAVEKER
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFATALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTVCRVPVDIPR
EALLEAGFKESDLNGTWKPYRPVGCSACSGGYKGRVGIYQVMPITEAIQEIILRDGSALDIAQQSEAEGVRSLRQSGLGK
VMQGLTSLEEVVACTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=404773 GNX71_RS26655 WP_206175203.1 5740251..5741984(+) (pilB) [Variovorax sp. RKNM96]
ATGGCCGCTGCCGAACTTCCAGTTAAAGAACTCTCGCAAATTGCCCTCCCGGGCTTGGCGCGCGCTCTCGTTTCGGCCGG
CAAACTGCCTGCAAAAATCGCAGAGGACATCTATCAGAAGTCCCTGAGCGGTCGCACCAGCTTCATTGCGGAACTGACCG
GCACCGGCGCCGTTTCGGCAGCGGATCTCGCCCACACGCTTTCCACCGCGTTCGGCGCCCCGCTGCTCGATCTCGATGCG
ATCGATCAACAGCGCCTGCCCAAGGACCTGCTTGATCCCAAGCTGTGCCTCGCGTACCGCATCGTCGTCCTCAGCAAGCG
CAACAACCGTCTCATTGTTGCAACGGCCGATCCATCGGACCAGCAGGCGGCGGAAAAGATCAAGTTCGCCTCCCAGATGG
GGGTCGACTGGGTCATCGCGGAGTACGACAAGCTCTCGCGAATGATCGAAGCCGCCGCAGCGACTGCAGCGGAGACCATC
AACAGCATCGTCGGCGCCGAGTTCGAATTCGACGATGTCGGCGCCGATACCTCGGGCGATGCCAGCGAACAAGCTGTTGC
CGAGGTCGAAGATGCGCCGGTCGTGCGCTTTCTGCACAAGATGCTGCTCGACGCCGTCAGCATGCGGGCCTCGGACATTC
ACTTCGAGCCCTACGAGCATCACTACCGCGTGCGTTTCCGGGTTGACGGCGAACTGCGCGAAATCGCGAGCCCGCCCACG
GTCATCAAGGACAAGCTGGCGTCACGCATCAAGGTCATTTCCCGGCTCGACATCTCCGAAAAACGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAAATCGGGCCGGACCGCGTGATCGACTTTCGCGTGAGCACGTTGCCCACGCTGTTCGGCGAGAAGA
TCGTGGTTCGTATTCTCGACCCCAGCAGTGCACGCCTGGGCATCGATGCGCTGGGCTACGACGCCGTCGAAAAGGAACGG
CTCCTCCATGCGATCGGCCGCCCTTACGGCATGGTGCTCGTCACCGGCCCGACGGGCTCGGGCAAGACGGTGTCGCTCTA
CACCTGCCTGAACCTGCTGAACCAGCCGGGCGTCAACATCGCGACGGCGGAAGACCCGTCCGAAATCAACCTCCCCGGCG
TCAACCAGGTCAACGTCAACGAACGGGCCGGACTGACCTTCGCCACAGCGCTGCGCGCCTTCCTGCGCCAGGATCCCGAC
ATCATCATGGTCGGCGAAATTCGCGACCTCGAAACCGCCGATATCTCGATCAAGGCCGCGCAGACCGGTCACCTGGTGCT
CTCGACGCTGCATACCAACGACGCGCCGACCACGCTCACGCGCATGCGCAACATGGGCATCGCGCCGTTCAACATCGCAT
CGAGCGTGATCCTGATCACCGCGCAGCGGCTTGCGCGCCGCCTGTGCACCGTATGCCGTGTGCCGGTCGACATCCCGCGC
GAAGCGCTGCTGGAGGCCGGCTTCAAGGAGTCGGACCTGAACGGCACGTGGAAACCTTACCGGCCCGTGGGCTGCTCCGC
ATGCAGCGGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATCACCGAGGCCATCCAGGAAATCATCCTGC
GCGATGGCAGCGCGCTCGACATCGCGCAGCAATCCGAAGCCGAGGGCGTGCGTTCGCTGCGCCAGTCGGGCCTCGGGAAA
GTCATGCAGGGCCTCACTTCGCTCGAAGAAGTGGTGGCCTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-156)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.367

97.227

0.529
  pilB Acinetobacter baylyi ADP1

53.832

97.227

0.523
  pilF Neisseria gonorrhoeae MS11

52.566

97.92

0.515
  pilB Legionella pneumophila strain ERS1305867

49.464

97.054

0.48
  pilB Vibrio cholerae strain A1552

47.698

94.107

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

45.617

96.88

0.442
  pilB Vibrio campbellii strain DS40M4

45.276

97.227

0.44
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.073

95.147

0.381
  pilF Thermus thermophilus HB27

38.354

98.96

0.38

Multiple sequence alignment